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The map label for this gene is trmD

Identifier: 153935798

GI number: 153935798

Start: 2422744

End: 2423466

Strand: Reverse

Name: trmD

Synonym: CLC_2293

Alternate gene names: 153935798

Gene position: 2423466-2422744 (Counterclockwise)

Preceding gene: 153935028

Following gene: 153935032

Centisome position: 64.44

GC content: 30.84

Gene sequence:

>723_bases
ATGCGAATAGATGTGCTAACATTATTTCCAGAAATGTTTTCTATATTTAATCACAGCATAATAGGAAGAGCTATAGAAAA
GGAAATTTTAAAAATAAACACTGTAAATATAAGGGACTATACAATAGATAAACATAAAAAAGTAGATGATTATCCTTATG
GAGGGGGAGCTGGCATGGTAATGTCAGTACAGCCTATAGTTGATTCTATAAAAGCGGTGAAAAAAGAAAATAAAGGTAAA
GTTATATTTTTAGGACCTAAAGGGAAAACTTTTAATCAGAACTTGGCTAAAGAATTGGCCAAAGAGGAAGAACTAATATT
CTTATGTGGCCATTATGAAGGAATAGATGAACGGGCTTATGAATATATAGATATGGAAATATCTTTAGGAGATTTTGTTT
TAACAGGAGGAGAAATGGCTTGCATTCCTATAGTAGATTCTATATGTAGATTGGTAGACGGGGTGCTAGGAAGTAGTGAG
AGCTACGAGGATGAATCTTTTTACAATGGTCTTTTAGAATATCCTCAATATACAAGACCAGCTATATATGAAGGAAAATC
TGTGCCGGAGGTGCTTTTATCAGGTCACCATGAAAATATAAAAAAATGGCGAAAAGCTAAATCACTCATAATTACTGATA
AAGTAAGACCAGATCTATTTAAAAAATATAAATTAACAGAAGAAGATAAGAAAATATTAAAAGATTTTAATAAAAAATTA
TAG

Upstream 100 bases:

>100_bases
AAGGAAAGAGCGAGGTATTAGTACCAGCCATAAAAGATATAGTTACAAATATAGATATAGAAAACAAAACTGTAACTATA
AAACCTTTGGAGATATGGCA

Downstream 100 bases:

>100_bases
GAAAAATATTTTTTAATTGTAAAAAATATTGAATAAAAATTTTTACTATGGTAAAATGAATTTTGTGCTAGTACGGGCGG
TCCTCTGCTTATATCATTTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRIDVLTLFPEMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMSVQPIVDSIKAVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLGSSE
SYEDESFYNGLLEYPQYTRPAIYEGKSVPEVLLSGHHENIKKWRKAKSLIITDKVRPDLFKKYKLTEEDKKILKDFNKKL

Sequences:

>Translated_240_residues
MRIDVLTLFPEMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMSVQPIVDSIKAVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLGSSE
SYEDESFYNGLLEYPQYTRPAIYEGKSVPEVLLSGHHENIKKWRKAKSLIITDKVRPDLFKKYKLTEEDKKILKDFNKKL
>Mature_240_residues
MRIDVLTLFPEMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMSVQPIVDSIKAVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLGSSE
SYEDESFYNGLLEYPQYTRPAIYEGKSVPEVLLSGHHENIKKWRKAKSLIITDKVRPDLFKKYKLTEEDKKILKDFNKKL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=45.679012345679, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CLOB1 (A7FW09)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384620.1
- ProteinModelPortal:   A7FW09
- SMR:   A7FW09
- STRING:   A7FW09
- GeneID:   5397219
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2309
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   CBOT441770:CLB_2309-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27409; Mature: 27409

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVLTLFPEMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCEE
MSVQPIVDSIKAVKKENKGKVIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAY
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
EYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLGSSESYEDESFYNGLLEYPQYTRP
HHEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
AIYEGKSVPEVLLSGHHENIKKWRKAKSLIITDKVRPDLFKKYKLTEEDKKILKDFNKKL
CCCCCCCHHHHHHCCCHHHHHHHHHHHCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDVLTLFPEMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCEE
MSVQPIVDSIKAVKKENKGKVIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAY
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
EYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLGSSESYEDESFYNGLLEYPQYTRP
HHEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
AIYEGKSVPEVLLSGHHENIKKWRKAKSLIITDKVRPDLFKKYKLTEEDKKILKDFNKKL
CCCCCCCHHHHHHCCCHHHHHHHHHHHCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA