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The map label for this gene is cbiK [H]

Identifier: 153935693

GI number: 153935693

Start: 1025755

End: 1026537

Strand: Direct

Name: cbiK [H]

Synonym: CLC_1008

Alternate gene names: 153935693

Gene position: 1025755-1026537 (Clockwise)

Preceding gene: 153936266

Following gene: 153936017

Centisome position: 27.28

GC content: 26.82

Gene sequence:

>783_bases
ATGGATAAAAAAAAAAGAGCTATATTAGTGGTGAGCTTTGGTACTAGTTATGAACAATCATTAAAGGACTGTATAGAAAG
CACAGAAAATTTTATAAAAGAAAGTTTTAAAGATTATGATATAAAAAGAGCTTTTACTTCTTACATGATAATAAATAAAA
TATTAAAAAGAGATAGTATAAAGATAAATAATCCTATAGAAGCATTAGAAGAACTTAAGAAGGAAGGTTATGAAGAAGTT
ATAGTTCAACCTCTACATATAATGTTTGGAGAAGAGTATGAAAAAATAGAAAAAGCTGTTTTAGAATTTAAAGATAAATT
TAAGATATTAAAATTAGGAAAGCCTTTATTATTTAAAAATGAAGACTACAAAATAGCAACAGAAGCTTTAAAGACTCAAC
TTCCTAAATTAAATAAAGAGGAAGCTATAATACTTATGGGACATGGAACAGAACATCCAGCTAATGCTGCTTATTTTCAA
TTTGATTATTATTTAAGAGAACACATAAATCCTAATATATATCTTTGTAGTGTGGAAAGCGATCCTTCTATAAAATCAAT
AATACCAAAATTAAAGAAATCTGGAGTAAAAAATGTTTTACTAATGCCTTTTATGTTAGTGGCTGGAGATCATGCTAACA
ATGACATGGCAGGAGAGGATGAAGAATCCTGGAAAAATGTTTTGGAAAAGGAAGGCTTTAAAGTTGATTTATATTTAAAG
GGTCTAGGAGCAAATAGAGAATTTCAAAAAATATATAGAGATCATATAAAAGAAGAAATCTAA

Upstream 100 bases:

>100_bases
ACAAAAAAATTATAAATTTAAATTTACGCATATATTTAATGAAATATTTAAAAAATATGTTAATTAAATAATATTATTAG
TATAGATCAGGAGGAGAAAC

Downstream 100 bases:

>100_bases
GGAGAGTATTTCATGGAAGTTACAGCCAAATATCCAGGAAGTTTTGGGGAAATACTTCAAGGAAATTTAGGGGAAAAGCC
TGTGTTGGTTTCTTCCCCTA

Product: anaerobic sirohydrochlorin cobaltochelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDKKKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDSIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKDKFKILKLGKPLLFKNEDYKIATEALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGEDEESWKNVLEKEGFKVDLYLK
GLGANREFQKIYRDHIKEEI

Sequences:

>Translated_260_residues
MDKKKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDSIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKDKFKILKLGKPLLFKNEDYKIATEALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGEDEESWKNVLEKEGFKVDLYLK
GLGANREFQKIYRDHIKEEI
>Mature_260_residues
MDKKKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDSIKINNPIEALEELKKEGYEEV
IVQPLHIMFGEEYEKIEKAVLEFKDKFKILKLGKPLLFKNEDYKIATEALKTQLPKLNKEEAIILMGHGTEHPANAAYFQ
FDYYLREHINPNIYLCSVESDPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGEDEESWKNVLEKEGFKVDLYLK
GLGANREFQKIYRDHIKEEI

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 30210; Mature: 30210

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDSI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KINNPIEALEELKKEGYEEVIVQPLHIMFGEEYEKIEKAVLEFKDKFKILKLGKPLLFKN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EDYKIATEALKTQLPKLNKEEAIILMGHGTEHPANAAYFQFDYYLREHINPNIYLCSVES
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECC
DPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGEDEESWKNVLEKEGFKVDLYLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GLGANREFQKIYRDHIKEEI
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKKKRAILVVSFGTSYEQSLKDCIESTENFIKESFKDYDIKRAFTSYMIINKILKRDSI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KINNPIEALEELKKEGYEEVIVQPLHIMFGEEYEKIEKAVLEFKDKFKILKLGKPLLFKN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EDYKIATEALKTQLPKLNKEEAIILMGHGTEHPANAAYFQFDYYLREHINPNIYLCSVES
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECC
DPSIKSIIPKLKKSGVKNVLLMPFMLVAGDHANNDMAGEDEESWKNVLEKEGFKVDLYLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GLGANREFQKIYRDHIKEEI
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]