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The map label for this gene is thiM-2 [H]

Identifier: 153935485

GI number: 153935485

Start: 2312134

End: 2312931

Strand: Reverse

Name: thiM-2 [H]

Synonym: CLC_2176

Alternate gene names: 153935485

Gene position: 2312931-2312134 (Counterclockwise)

Preceding gene: 153935503

Following gene: 153934564

Centisome position: 61.5

GC content: 32.71

Gene sequence:

>798_bases
TTGCAAATAAGACAGAGTATTAAATTAAAAAAACCTCTCATTCACTATATTACAAATCCAATTTCAATAAATGATTGTGC
AAATATAATTCTTGCTGCTGGCGCAAAGCCCATTATGGCCGAGCATCCTTTGGAAGTTTCCGAAATCACTTCTGTTTCAA
AATCTCTTGGAGTTAATCTTGGGAATATAACAGATAATAAAATGAAATCTATGTTGATTTCAGGTAAAACAGCCTATGAA
AATAAGATTCCGCAAGTAATTGATCTTGTAGGCGTAGGTTGCAGTAAACTTCGTCTAGATTATGCAAAGAAATTTATTTC
AGAGTGTCATCCAAATGTTATTAAAGGTAATATGTCCGAAATAAAAGCAATCTATGGCATAAAAAGTAGTGCAAAAGGAA
TTGATGTTGGAGCATGCGATATTATAACAAAGCAAAATTTTGATGAAAATATAGAAATGATAAAGAGATTATCCATGGAA
ACCGGCTCTGTTGTTGCAGCTACAGGCGTGGTGGATATAATAAGTAATGGTACTTATACATATATAATATCAAATGGTTG
TGAAATGCTCTCAATGATAACTGGGACAGGCTGTATGCTTACTGGGCTTATAGCAAGCTATATTTCTTCTGAGAATATAT
TAGATGGTACTGTTCTTGCTGTGGCTCTTATGGGAATATGTGGTGAACTTTCTCAGCATGCTAAGGGTACTGGAAGCTTC
AGAAATGAGCTTACAGATAATATGTTTAGTATTTCCGATGATATTATAATAAAAAAAATAAGAATAAATAGTTATTAA

Upstream 100 bases:

>100_bases
TTTCTAGGAGCATTAAGTAGGACAGGTAATCTTACAAAAATTCAAAGAAGTTTGGGGGCAAAAATTTATGATAAAGCAAG
AATTAATAATAAACAAACTA

Downstream 100 bases:

>100_bases
TTATAGATACTGGATTTTTATTTTACTATATTTCCAATCTGAATATAGTTAATAGCTAATTTTTGTATAAGTGAAACTAT
AAGAAGCTTTAAATAGTTTA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQIRQSIKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFISECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITKQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGLIASYISSENILDGTVLAVALMGICGELSQHAKGTGSF
RNELTDNMFSISDDIIIKKIRINSY

Sequences:

>Translated_265_residues
MQIRQSIKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFISECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITKQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGLIASYISSENILDGTVLAVALMGICGELSQHAKGTGSF
RNELTDNMFSISDDIIIKKIRINSY
>Mature_265_residues
MQIRQSIKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFISECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITKQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGLIASYISSENILDGTVLAVALMGICGELSQHAKGTGSF
RNELTDNMFSISDDIIIKKIRINSY

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=28.740157480315, Blast_Score=114, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325042, Length=269, Percent_Identity=30.4832713754647, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28577; Mature: 28577

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRQSIKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHCCCC
GNITDNKMKSMLISGKTAYENKIPQVIDLVGVGCSKLRLDYAKKFISECHPNVIKGNMSE
CCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCHHH
IKAIYGIKSSAKGIDVGACDIITKQNFDENIEMIKRLSMETGSVVAATGVVDIISNGTYT
HHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEE
YIISNGCEMLSMITGTGCMLTGLIASYISSENILDGTVLAVALMGICGELSQHAKGTGSF
EEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
RNELTDNMFSISDDIIIKKIRINSY
HHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MQIRQSIKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHCCCC
GNITDNKMKSMLISGKTAYENKIPQVIDLVGVGCSKLRLDYAKKFISECHPNVIKGNMSE
CCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCHHH
IKAIYGIKSSAKGIDVGACDIITKQNFDENIEMIKRLSMETGSVVAATGVVDIISNGTYT
HHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEE
YIISNGCEMLSMITGTGCMLTGLIASYISSENILDGTVLAVALMGICGELSQHAKGTGSF
EEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
RNELTDNMFSISDDIIIKKIRINSY
HHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA