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The map label for this gene is rnhB

Identifier: 153935357

GI number: 153935357

Start: 2419378

End: 2420187

Strand: Reverse

Name: rnhB

Synonym: CLC_2289

Alternate gene names: 153935357

Gene position: 2420187-2419378 (Counterclockwise)

Preceding gene: 153936572

Following gene: 153936174

Centisome position: 64.36

GC content: 27.78

Gene sequence:

>810_bases
ATGAATTTAAATAATTTAGAAAATATACGATATAATGAAATAAAGGAATTCTCAGATAAAATTAAAAAAGAATTTACATT
TTCTCAAAAAAAACAGGTTATGGACATAATAGAAAAATTAAATAAAGATTCTCGGAAAAACGTTATAAAGTTAGGGCAGG
CTCTTGAAAAATTTTTAAATAAGTATGAAGAAGAGTTGAAAAGAACAAATAATATGTATAATTTCGATAGAAGATATGGC
AACAATTATTTAATAGCAGGAGTAGATGAGGTTGGAAGAGGACCTTTAGCAGGACCTATTGTAGCCGCTGCGGTGGTTTT
AGATTTAAATGTGGAGGAAATGCAAAGGGTTTTTAATATAAAGGACTCAAAAAAGCTTTCAGAAAAAAAGAGAGAAGAAT
TAGATATAATAATAAGAGAAAAAGCTATAAGCTACAATATAGCCTTAGTAGATAATAAAACAATAGATGAAAGAGGTATA
TCTTGGAGTAATAATGAAGTGCTTAAAAGAGCAGTAGAAGGGCTAAAGGTAAAGCCTGATTTAGTTTTATCTGATGGATA
TGCAGTTAAAAATTTGAATATAAGAAATGAATTTATAATAAAAGGTGATAGTAAAAGTATTAGTATAGCATCTTCATCCA
TTATAGCTAAGGTATATAGAGACAGTATGATGAAGGAATATTCTAAAGGGCTAAATATGTATGGTTTCAATCATAATGCA
GGTTATGGTACAGAAGAACATGTACAAGCAATAAAAAAACATGGACCTTCTAAAATACATAGAATGAGTTTTTTAACAAA
TATATTGTAA

Upstream 100 bases:

>100_bases
GGTTATAATATTTCCTACATTATAGTTAATAAGTATTATTTTAGGATAAATATTAAATAAAAATAAATACTAGAAAGTAA
ATTAGTATAGGAGTACAGTT

Downstream 100 bases:

>100_bases
TTAAATAAATAAAAAACGGTTTCTCAAAATAGATTCGATTTTGACAGCCCCAATATAAAAATACACTTATTAGGTGTATT
TTTTATATTTGAAAAGCGTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNLNNLENIRYNEIKEFSDKIKKEFTFSQKKQVMDIIEKLNKDSRKNVIKLGQALEKFLNKYEEELKRTNNMYNFDRRYG
NNYLIAGVDEVGRGPLAGPIVAAAVVLDLNVEEMQRVFNIKDSKKLSEKKREELDIIIREKAISYNIALVDNKTIDERGI
SWSNNEVLKRAVEGLKVKPDLVLSDGYAVKNLNIRNEFIIKGDSKSISIASSSIIAKVYRDSMMKEYSKGLNMYGFNHNA
GYGTEEHVQAIKKHGPSKIHRMSFLTNIL

Sequences:

>Translated_269_residues
MNLNNLENIRYNEIKEFSDKIKKEFTFSQKKQVMDIIEKLNKDSRKNVIKLGQALEKFLNKYEEELKRTNNMYNFDRRYG
NNYLIAGVDEVGRGPLAGPIVAAAVVLDLNVEEMQRVFNIKDSKKLSEKKREELDIIIREKAISYNIALVDNKTIDERGI
SWSNNEVLKRAVEGLKVKPDLVLSDGYAVKNLNIRNEFIIKGDSKSISIASSSIIAKVYRDSMMKEYSKGLNMYGFNHNA
GYGTEEHVQAIKKHGPSKIHRMSFLTNIL
>Mature_269_residues
MNLNNLENIRYNEIKEFSDKIKKEFTFSQKKQVMDIIEKLNKDSRKNVIKLGQALEKFLNKYEEELKRTNNMYNFDRRYG
NNYLIAGVDEVGRGPLAGPIVAAAVVLDLNVEEMQRVFNIKDSKKLSEKKREELDIIIREKAISYNIALVDNKTIDERGI
SWSNNEVLKRAVEGLKVKPDLVLSDGYAVKNLNIRNEFIIKGDSKSISIASSSIIAKVYRDSMMKEYSKGLNMYGFNHNA
GYGTEEHVQAIKKHGPSKIHRMSFLTNIL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=186, Percent_Identity=40.8602150537634, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CLOB1 (A7FW05)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384616.1
- ProteinModelPortal:   A7FW05
- SMR:   A7FW05
- STRING:   A7FW05
- GeneID:   5398002
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2305
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   CBOT441770:CLB_2305-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 30915; Mature: 30915

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNNLENIRYNEIKEFSDKIKKEFTFSQKKQVMDIIEKLNKDSRKNVIKLGQALEKFLN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KYEEELKRTNNMYNFDRRYGNNYLIAGVDEVGRGPLAGPIVAAAVVLDLNVEEMQRVFNI
HHHHHHHHHCCCCHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCC
KDSKKLSEKKREELDIIIREKAISYNIALVDNKTIDERGISWSNNEVLKRAVEGLKVKPD
CCHHHHHHHHHHHHHHHHEEHHEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCC
LVLSDGYAVKNLNIRNEFIIKGDSKSISIASSSIIAKVYRDSMMKEYSKGLNMYGFNHNA
EEEECCEEEEECCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
GYGTEEHVQAIKKHGPSKIHRMSFLTNIL
CCCCHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLNNLENIRYNEIKEFSDKIKKEFTFSQKKQVMDIIEKLNKDSRKNVIKLGQALEKFLN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KYEEELKRTNNMYNFDRRYGNNYLIAGVDEVGRGPLAGPIVAAAVVLDLNVEEMQRVFNI
HHHHHHHHHCCCCHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCC
KDSKKLSEKKREELDIIIREKAISYNIALVDNKTIDERGISWSNNEVLKRAVEGLKVKPD
CCHHHHHHHHHHHHHHHHEEHHEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCC
LVLSDGYAVKNLNIRNEFIIKGDSKSISIASSSIIAKVYRDSMMKEYSKGLNMYGFNHNA
EEEECCEEEEECCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
GYGTEEHVQAIKKHGPSKIHRMSFLTNIL
CCCCHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA