Click here to switch to the map view.

The map label for this gene is cbiT [H]

Identifier: 153935333

GI number: 153935333

Start: 1016247

End: 1016822

Strand: Direct

Name: cbiT [H]

Synonym: CLC_0994

Alternate gene names: 153935333

Gene position: 1016247-1016822 (Clockwise)

Preceding gene: 153936317

Following gene: 153934978

Centisome position: 27.02

GC content: 28.82

Gene sequence:

>576_bases
ATGAGATATATAAAGGATGAAGAATTTATAAGGGGAAGTTGTCCTATGACTAAAGAGGACATAAGAATTTTATCCATATC
AAAGATGAACTTAGAAGAAAATTCAAAGGTTTTAGATGTGGGTGCAGGTACAGGAAGTGTAAGTATACAAGCAGCTACAA
TATGTGCAAAAGGACAAGTTTTTGCCATAGAAAAAGATGAGGAAGCCTTAGACATTATAAAAAAAAATAAAGAAAAATTT
AATTGTAAAAATCTAAAGATAATAGAGGGAGAAGCCTTAAAAGTAGAAGAAAGTATAAAGGATAGTTTTGATAGTATATT
TATAGGTGGTAGCGGTGGAGGTCTGGAAGAAATAATAAGTAGATATGGGAACAAGCTTTTAGACCAAGGCACAATGGTTT
TAAATTTTATAACCATAAACAATTTAAATACGGCGTTAGAAACCTTAAAAAATTTAAATTATAAAACAGAATGTATTCAA
GTTTCTATAAGTAAAGCTAGAGGAAAGTCAAATATGCTTATTGCTAATAATCCTATATTTATAATAACAGCTATTAAAAA
TGGAGGGAATTTATAA

Upstream 100 bases:

>100_bases
ATAAAATTTATACTAAATAAGTAAAATTTGAGAAGTAAATGTCTATTATAATATAAAACAAATTACTTTTAGTGGCTAAA
TATATAATAGGGGGAGTAGG

Downstream 100 bases:

>100_bases
TGAATAAAATAAAAAAAATTATAGTTTCAAGTGTGGTTATCTGTTTGTTAGGTGGTATTGGGATTTATAAATATATAGAT
ACAAGCCATAAAAAAGACCT

Product: precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRYIKDEEFIRGSCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAATICAKGQVFAIEKDEEALDIIKKNKEKF
NCKNLKIIEGEALKVEESIKDSFDSIFIGGSGGGLEEIISRYGNKLLDQGTMVLNFITINNLNTALETLKNLNYKTECIQ
VSISKARGKSNMLIANNPIFIITAIKNGGNL

Sequences:

>Translated_191_residues
MRYIKDEEFIRGSCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAATICAKGQVFAIEKDEEALDIIKKNKEKF
NCKNLKIIEGEALKVEESIKDSFDSIFIGGSGGGLEEIISRYGNKLLDQGTMVLNFITINNLNTALETLKNLNYKTECIQ
VSISKARGKSNMLIANNPIFIITAIKNGGNL
>Mature_191_residues
MRYIKDEEFIRGSCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAATICAKGQVFAIEKDEEALDIIKKNKEKF
NCKNLKIIEGEALKVEESIKDSFDSIFIGGSGGGLEEIISRYGNKLLDQGTMVLNFITINNLNTALETLKNLNYKTECIQ
VSISKARGKSNMLIANNPIFIITAIKNGGNL

Specific function: Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12 [H]

COG id: COG2242

COG function: function code H; Precorrin-6B methylase 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Archaeal-type CbiT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014008
- InterPro:   IPR002935
- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 20984; Mature: 20984

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIKDEEFIRGSCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAATICAKGQV
CCCCCCCCEECCCCCCCHHCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEECCEE
FAIEKDEEALDIIKKNKEKFNCKNLKIIEGEALKVEESIKDSFDSIFIGGSGGGLEEIIS
EEEECCCHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHCCCCEEEEECCCCCHHHHHH
RYGNKLLDQGTMVLNFITINNLNTALETLKNLNYKTECIQVSISKARGKSNMLIANNPIF
HHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCEE
IITAIKNGGNL
EEEEECCCCCC
>Mature Secondary Structure
MRYIKDEEFIRGSCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAATICAKGQV
CCCCCCCCEECCCCCCCHHCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEECCEE
FAIEKDEEALDIIKKNKEKFNCKNLKIIEGEALKVEESIKDSFDSIFIGGSGGGLEEIIS
EEEECCCHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHCCCCEEEEECCCCCHHHHHH
RYGNKLLDQGTMVLNFITINNLNTALETLKNLNYKTECIQVSISKARGKSNMLIANNPIF
HHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCEE
IITAIKNGGNL
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA