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The map label for this gene is ispG [H]

Identifier: 153934543

GI number: 153934543

Start: 2402313

End: 2403362

Strand: Reverse

Name: ispG [H]

Synonym: CLC_2271

Alternate gene names: 153934543

Gene position: 2403362-2402313 (Counterclockwise)

Preceding gene: 153937005

Following gene: 153935522

Centisome position: 63.91

GC content: 31.71

Gene sequence:

>1050_bases
ATGATGAGAAAATCAACTAAAAAAGTAAAAGTAGGAAGTATATATGTAGGAGGAGATTCTCCTATATCTATACAATCCAT
GACAAATACAGATACTAGAGATGTTAAAAGTACACTAAATCAAATAAATAAGTTAGAAAAAATAGGCTGTGATATAATAA
GATGCGCAGTACCAGATATAGAAGCTAGTGAAGCTTTAAAAATAATAACCAAGGAATCAAAAATTCCAGTGGTGGCAGAT
ATACATTTTGATCATAAATTAGCCTTAGAATCTATAAAAAATGGAGTAGATGCCTTAAGAATAAACCCAGGAAATATAGG
GTCCATGGAAAGGGTTAAGATGGTAGCAGAAGCTGCAAAGGGAAAATCTATTCCTATAAGGGTAGGAGTAAACTCAGGTT
CCTTAAAAAAAGATATATTAGATAAATATGGTAGAGTATGCCCAGAAGCATTAGTAGAAAGTGCCCTTCAGCATGTGAAC
ATATTAGAAAAATGCAATTTTAATGATATAGTAATATCTATAAAGTCTTCTAATGTTATACAAATGATAGAAAGTTATAG
ATTAATATCAGAAAAAGTAAATTATCCTTTGCATTTAGGAGTTACAGAAGCAGGAACTACCTTTAGAGGAACTATAAAAT
CTAGTGTAGGTATAGGTACCCTGCTGGCAGAAGGTATAGGAGATACTATAAGAGTATCTATTACAGGAGATCCGCTAGAA
GAAATTAAAATAGGAAAAGAAATATTGAGATCCTTAGGGTATGTAAATGAAGGAATAGAATTTGTATCTTGTCCAACTTG
TGGAAGAACGAATATAGATTTAATATCTATAGCTGAAGAGGTAGAAAAAAGGCTTTTAAACTGTAATAAGAATATTAAAG
TAGCGGTAATGGGATGCGTAGTTAATGGGCCTGGGGAAGCAAGGGAAGCAGATATAGGTATAGCAGGAGGTAAAGGAGAA
GGGCTTATATTCAAAAAGGGAGAGGTTATAAAAAAAGTTAAAGAAAAGAATATAATAGATGAACTTATAAAAGAAATAGA
AAAGATGTAA

Upstream 100 bases:

>100_bases
AAATTTCATCGAAATTTAAGGATAACTTTATAAAAAACTTTGCTTAACATTATAAATTCATTGATTTTATTAGGGAAGTT
TAAAACTAGGAGATGAAACA

Downstream 100 bases:

>100_bases
TTTGTAAATGTTTTAGTTTGCTTAAATTAATTTGGATTTCAACATACATAGAAGATAGAGTATATAAAAATTTCTTAGCT
TTAGGTAGTAGGGTTTTAGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSNVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAEEVEKRLLNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEKNIIDELIKEIEKM

Sequences:

>Translated_349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSNVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAEEVEKRLLNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEKNIIDELIKEIEKM
>Mature_349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSNVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAEEVEKRLLNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEKNIIDELIKEIEKM

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=342, Percent_Identity=47.3684210526316, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37855; Mature: 37855

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDI
CCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
EASEALKIITKESKIPVVADIHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAK
CHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
GKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVNILEKCNFNDIVISIKSSNVI
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
QMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCCCCHHHHHHHCCCCEEEEEECCCCHH
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAEEVEKRLLNCNKNIKVAVMGCV
HHHHHHHHHHHHCCHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFKKGEVIKKVKEKNIIDELIKEIEKM
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDI
CCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
EASEALKIITKESKIPVVADIHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAK
CHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
GKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVNILEKCNFNDIVISIKSSNVI
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
QMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCCCCHHHHHHHCCCCEEEEEECCCCHH
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAEEVEKRLLNCNKNIKVAVMGCV
HHHHHHHHHHHHCCHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFKKGEVIKKVKEKNIIDELIKEIEKM
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]