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The map label for this gene is dctA [H]

Identifier: 153934521

GI number: 153934521

Start: 639292

End: 640593

Strand: Direct

Name: dctA [H]

Synonym: CLC_0622

Alternate gene names: 153934521

Gene position: 639292-640593 (Clockwise)

Preceding gene: 153936910

Following gene: 153936878

Centisome position: 17.0

GC content: 31.41

Gene sequence:

>1302_bases
ATGGAAGTAAAAATAGATTCTATAAAGTTATATATATTTGGGGGAGATAGAATGAAAGTATGGGAAAATTATAAATGGTC
TATAATACTACTTATTTCTGTAATTATAGGAGGAATAGTTGGATTAATTATGGGACCAAGAGCTGAAGTAGTAGAACCTT
TAGGTAACTTATTTTTAAATTTAATGTTTACTGCAATTGTGCCTCTAGTATTTTTTAGTGTGGCTTCAGCTATTGCTAAT
ATGGCTAACATGAAAAGACTAGGAAAGATTTTATTAAGCGTATTTATAGTATTTACTTTTACCGCATTAATTGCAGCAGT
AATTGGTGTTATAGGAACCTTAATAATAAACCCTACAAAGGGTGTAGATCCTTCCACTATGCAAAAGATAATGGCAAATA
CAGTTAATGAAATTCAAAATAAAGAGAAGGTAAGTATGTTACAACAAATTGTAAATACAGTTACAGTATCGGATTTTGCA
TTATTATTTTCTAGAAAAAATATGCTACAAATTATTATATTTTCAATAATCTTTGGTGTTTCTACAGCTATGGTTGGAGA
AAAGGGTAAACTTGTTGCTAAGTTTTTAGAAGCTGGTTCAACTGTAATGATAAAAATGATAGATATAATAATGTATACAG
CACCAATAGGACTTGGTTGTTATTTTGCTTCAGTAATAGGACAACTTGGTCCACAAATAATTGAAGGATATGTGAGAGTG
TTTATATTATATTTGATACTTACTTTAGTATATTATTTTATTATGTTTAGCATATATGCATATATGTCAGCAGGAAAAAC
AGGAGTGCAACTATTTTGGAAGAATGCATTGGCGCCTTCAATAACATCCCTTGCTACCTGTTCTAGTGCAGCATGTATTC
CTGTTAATTTAGAAAGCACAAAAAATATGGGAGTACCAAAGGATATTAGAGAGACAGTTATACCCCTAGGAGCAAACACT
CATAAAGATGGATCTGTTATAGGTGGAGTTTTAAAAATAACTTTTTTACTTGGATTATTTGGTCATGATATGACTAGTTT
TAAAGCAATAATTACAATAATTATAGTGTCTTATTTAATAGGAGCTGTTATGGGAGCTATCCCTGGTGGAGGACTTATAG
GTGCTATGCTCATCATAAGCATATATGGCTTTGCCCCTGAAGTTTTAGGAATAGTGGCAGTCATAAGCACTATAATAGAT
GCTCCAGCAACGCTATTAAATTCTACAGGAAATACGGTATGTGCTATGATGATAACAAGACTTGTGGAAGGAAAAGATTG
GGTTAAAAAAATTATAGCATAG

Upstream 100 bases:

>100_bases
ATAAATTAAGAAGTATACATTATTAGACAAGTATTTTTAAGCTTAAGTTCAAGGTAGAATTCTATAGGAAATAAAGAACT
TTTGTTATTAGATTTGGATT

Downstream 100 bases:

>100_bases
AAATTTTTAAAGGTTAAATATTAAAACAAGAAATACTTTTAGTCAATAGATAGCTATAAAAATAAAGAATACTAATATTT
GTGTCCTAATATTTAAGCTA

Product: proton/sodium-glutamate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MEVKIDSIKLYIFGGDRMKVWENYKWSIILLISVIIGGIVGLIMGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIAN
MANMKRLGKILLSVFIVFTFTALIAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEIQNKEKVSMLQQIVNTVTVSDFA
LLFSRKNMLQIIIFSIIFGVSTAMVGEKGKLVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRV
FILYLILTLVYYFIMFSIYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTKNMGVPKDIRETVIPLGANT
HKDGSVIGGVLKITFLLGLFGHDMTSFKAIITIIIVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIID
APATLLNSTGNTVCAMMITRLVEGKDWVKKIIA

Sequences:

>Translated_433_residues
MEVKIDSIKLYIFGGDRMKVWENYKWSIILLISVIIGGIVGLIMGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIAN
MANMKRLGKILLSVFIVFTFTALIAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEIQNKEKVSMLQQIVNTVTVSDFA
LLFSRKNMLQIIIFSIIFGVSTAMVGEKGKLVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRV
FILYLILTLVYYFIMFSIYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTKNMGVPKDIRETVIPLGANT
HKDGSVIGGVLKITFLLGLFGHDMTSFKAIITIIIVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIID
APATLLNSTGNTVCAMMITRLVEGKDWVKKIIA
>Mature_433_residues
MEVKIDSIKLYIFGGDRMKVWENYKWSIILLISVIIGGIVGLIMGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIAN
MANMKRLGKILLSVFIVFTFTALIAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEIQNKEKVSMLQQIVNTVTVSDFA
LLFSRKNMLQIIIFSIIFGVSTAMVGEKGKLVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRV
FILYLILTLVYYFIMFSIYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTKNMGVPKDIRETVIPLGANT
HKDGSVIGGVLKITFLLGLFGHDMTSFKAIITIIIVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIID
APATLLNSTGNTVCAMMITRLVEGKDWVKKIIA

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=474, Percent_Identity=21.9409282700422, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI223468564, Length=263, Percent_Identity=22.4334600760456, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI223468566, Length=263, Percent_Identity=22.4334600760456, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI169790839, Length=257, Percent_Identity=23.3463035019455, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI5032093, Length=260, Percent_Identity=22.6923076923077, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI21314632, Length=278, Percent_Identity=23.3812949640288, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4827012, Length=268, Percent_Identity=23.134328358209, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI66773030, Length=277, Percent_Identity=25.2707581227437, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194239697, Length=473, Percent_Identity=20.2959830866808, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI262359914, Length=209, Percent_Identity=24.8803827751196, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=26.8115942028986, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790514, Length=394, Percent_Identity=24.3654822335025, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788024, Length=292, Percent_Identity=25.3424657534247, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193206505, Length=436, Percent_Identity=21.5596330275229, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537407, Length=432, Percent_Identity=22.6851851851852, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71983099, Length=434, Percent_Identity=20.7373271889401, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983106, Length=434, Percent_Identity=20.7373271889401, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996953, Length=423, Percent_Identity=21.2765957446809, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI193206654, Length=412, Percent_Identity=23.0582524271845, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583025, Length=437, Percent_Identity=20.3661327231121, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137668, Length=437, Percent_Identity=20.3661327231121, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583023, Length=437, Percent_Identity=20.3661327231121, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46653; Mature: 46653

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKIDSIKLYIFGGDRMKVWENYKWSIILLISVIIGGIVGLIMGPRAEVVEPLGNLFLN
CCEEEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIVFTFTALIAAVIGVIGTLIINPTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVDPSTMQKIMANTVNEIQNKEKVSMLQQIVNTVTVSDFALLFSRKNMLQIIIFSIIFGV
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STAMVGEKGKLVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRV
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
FILYLILTLVYYFIMFSIYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLEST
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCC
KNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLLGLFGHDMTSFKAIITIIIVSYLI
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMMITR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHH
LVEGKDWVKKIIA
HHCCHHHHHHHCC
>Mature Secondary Structure
MEVKIDSIKLYIFGGDRMKVWENYKWSIILLISVIIGGIVGLIMGPRAEVVEPLGNLFLN
CCEEEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIVFTFTALIAAVIGVIGTLIINPTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVDPSTMQKIMANTVNEIQNKEKVSMLQQIVNTVTVSDFALLFSRKNMLQIIIFSIIFGV
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STAMVGEKGKLVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRV
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
FILYLILTLVYYFIMFSIYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLEST
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCC
KNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLLGLFGHDMTSFKAIITIIIVSYLI
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMMITR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHH
LVEGKDWVKKIIA
HHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]