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The map label for this gene is ecoRIIR [H]

Identifier: 148244083

GI number: 148244083

Start: 31098

End: 32336

Strand: Reverse

Name: ecoRIIR [H]

Synonym: Acry_3581

Alternate gene names: 148244083

Gene position: 32336-31098 (Counterclockwise)

Preceding gene: 148244084

Following gene: 148244080

Centisome position: 86.43

GC content: 61.9

Gene sequence:

>1239_bases
ATGGCGCTGACTGATCTTGTGGGCTGGATGGACGAGTACGGCGTCCCCGGCGCTGTCTGGTTCGCCAAGCGGCTGGCAGC
CAACGATACCCTGGCAACCGGAGCCCATGGAGCGGGGCCCTATATTCCAAAGGAGTTCCTGTTCAGCGTTTTCCCGGACC
TCAACCACCCAGAGGAGGAGAATCCGGACTGCTGGTTCGACCTGTACATCGACTCTCACGCGGACCGCCGGAGGATCCGG
GCGATCTGGTACAACAACAGGCTTCGTGGAGGGACGAGGAACGAAGCCCGACTGACGAATTTCGGCGGTGGCCGGTCAGC
ACTTCTGGATCCGGACAGTACCGGTGCCCTGGCCGTGTTTGCCTTCCTCCCTCACGGGCCGGGCAGGCAGATGGAATGTC
ATGTCTGGGTCTGCGATCAGGGGGCGGAGGCAGATCTTCTTGAAGAGCGCATTGGTCCGGTAGAGCCGAAGGCTCCTGTG
GTCTGGCAGCCGGGCGAAATCGCCCGCGACCTGTTCATGCACACGGATGCAGGACCGGGGTCGTGCCGGCTCCTGCCCGG
TGAAATTCCTCCTGCCTGGCTGACCAGGTTCCCGACGGGCGAGGAAATTATCAGGGAGACGGTCAAACGACGCCAGCGAA
ACGGTCTGGAGCCCGACCGGCTTCTCATGCGCCGCCGTGAATGCGAATTTGAGATATTTCTCAGCGTCGAGGAGGCCGTC
TATCTCCCACGTATCAGGCAAGGCTTCTCCGCGATCGGCGATTTTCTCTCGCTTGCCCAGACAATCCTGCAGAGCCGGAA
GACCCGTTCAGGCACTTCGCTGGAACTGCATGTCCGCGAGATCATGACCGAGGAAGGTCTGCTGGCTGATACCTCCTTCA
CCTACCGGCCAGTAATCGAGAACGGCAAGCGGCCGGATTTCCTGTTCCCGTCCAAGGCCGCCTACGACAATCCGGCGTTT
CCGGCAGAGCGGTTGCGCATGCTGGCCGCGAAAACGACCTGCAAGGACCGCTGGCGGCAGGTGCTCAACGAGGCCGACAG
GATCGCAACCAAGCACCTCCTCACCCTCCAGGAAGGGGTTTCGGAGGGGCAGTTCCGGGAAATGAGCGAGGCCGGCGTCC
GTCTGGTGGTGCCTGCTGAACTCCACCGTTCCTATCCGGCGAGTGTCCGGCCGCATCTTGTCACGTTCGAGAACTTCATT
GGTGACGTGAGACCGCTGTCCGCCATTCAGGGGCCATAG

Upstream 100 bases:

>100_bases
GCGATCAGGCGCAGGCGCGGCATGGTGCCGGAACCTGTCATTGACGAAGCCGTCTCGTGGCTGAGATCCGGAGATGGAAA
CCTGGAAATTCGGGGGAACA

Downstream 100 bases:

>100_bases
TCCCACAGGATCAGGCTGCCCGGTCTATCGGGTATGCTTTCACATCGGCCGGCCGCTTGCCCGCATACCAGGCGGGATCC
GGCAGCCGCCCCGATGTGAC

Product: hypothetical protein

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MALTDLVGWMDEYGVPGAVWFAKRLAANDTLATGAHGAGPYIPKEFLFSVFPDLNHPEEENPDCWFDLYIDSHADRRRIR
AIWYNNRLRGGTRNEARLTNFGGGRSALLDPDSTGALAVFAFLPHGPGRQMECHVWVCDQGAEADLLEERIGPVEPKAPV
VWQPGEIARDLFMHTDAGPGSCRLLPGEIPPAWLTRFPTGEEIIRETVKRRQRNGLEPDRLLMRRRECEFEIFLSVEEAV
YLPRIRQGFSAIGDFLSLAQTILQSRKTRSGTSLELHVREIMTEEGLLADTSFTYRPVIENGKRPDFLFPSKAAYDNPAF
PAERLRMLAAKTTCKDRWRQVLNEADRIATKHLLTLQEGVSEGQFREMSEAGVRLVVPAELHRSYPASVRPHLVTFENFI
GDVRPLSAIQGP

Sequences:

>Translated_412_residues
MALTDLVGWMDEYGVPGAVWFAKRLAANDTLATGAHGAGPYIPKEFLFSVFPDLNHPEEENPDCWFDLYIDSHADRRRIR
AIWYNNRLRGGTRNEARLTNFGGGRSALLDPDSTGALAVFAFLPHGPGRQMECHVWVCDQGAEADLLEERIGPVEPKAPV
VWQPGEIARDLFMHTDAGPGSCRLLPGEIPPAWLTRFPTGEEIIRETVKRRQRNGLEPDRLLMRRRECEFEIFLSVEEAV
YLPRIRQGFSAIGDFLSLAQTILQSRKTRSGTSLELHVREIMTEEGLLADTSFTYRPVIENGKRPDFLFPSKAAYDNPAF
PAERLRMLAAKTTCKDRWRQVLNEADRIATKHLLTLQEGVSEGQFREMSEAGVRLVVPAELHRSYPASVRPHLVTFENFI
GDVRPLSAIQGP
>Mature_411_residues
ALTDLVGWMDEYGVPGAVWFAKRLAANDTLATGAHGAGPYIPKEFLFSVFPDLNHPEEENPDCWFDLYIDSHADRRRIRA
IWYNNRLRGGTRNEARLTNFGGGRSALLDPDSTGALAVFAFLPHGPGRQMECHVWVCDQGAEADLLEERIGPVEPKAPVV
WQPGEIARDLFMHTDAGPGSCRLLPGEIPPAWLTRFPTGEEIIRETVKRRQRNGLEPDRLLMRRRECEFEIFLSVEEAVY
LPRIRQGFSAIGDFLSLAQTILQSRKTRSGTSLELHVREIMTEEGLLADTSFTYRPVIENGKRPDFLFPSKAAYDNPAFP
AERLRMLAAKTTCKDRWRQVLNEADRIATKHLLTLQEGVSEGQFREMSEAGVRLVVPAELHRSYPASVRPHLVTFENFIG
DVRPLSAIQGP

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 46432; Mature: 46301

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDLVGWMDEYGVPGAVWFAKRLAANDTLATGAHGAGPYIPKEFLFSVFPDLNHPEEE
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
NPDCWFDLYIDSHADRRRIRAIWYNNRLRGGTRNEARLTNFGGGRSALLDPDSTGALAVF
CCCEEEEEEECCCCCHHHEEEEEECCCCCCCCCCCCEEECCCCCCCEEECCCCCCCEEEE
AFLPHGPGRQMECHVWVCDQGAEADLLEERIGPVEPKAPVVWQPGEIARDLFMHTDAGPG
EECCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCCCC
SCRLLPGEIPPAWLTRFPTGEEIIRETVKRRQRNGLEPDRLLMRRRECEFEIFLSVEEAV
CEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEHHHHH
YLPRIRQGFSAIGDFLSLAQTILQSRKTRSGTSLELHVREIMTEEGLLADTSFTYRPVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCEECCCCEECHHHC
NGKRPDFLFPSKAAYDNPAFPAERLRMLAAKTTCKDRWRQVLNEADRIATKHLLTLQEGV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEGQFREMSEAGVRLVVPAELHRSYPASVRPHLVTFENFIGDVRPLSAIQGP
CCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCEEEHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
ALTDLVGWMDEYGVPGAVWFAKRLAANDTLATGAHGAGPYIPKEFLFSVFPDLNHPEEE
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
NPDCWFDLYIDSHADRRRIRAIWYNNRLRGGTRNEARLTNFGGGRSALLDPDSTGALAVF
CCCEEEEEEECCCCCHHHEEEEEECCCCCCCCCCCCEEECCCCCCCEEECCCCCCCEEEE
AFLPHGPGRQMECHVWVCDQGAEADLLEERIGPVEPKAPVVWQPGEIARDLFMHTDAGPG
EECCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCCCC
SCRLLPGEIPPAWLTRFPTGEEIIRETVKRRQRNGLEPDRLLMRRRECEFEIFLSVEEAV
CEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEHHHHH
YLPRIRQGFSAIGDFLSLAQTILQSRKTRSGTSLELHVREIMTEEGLLADTSFTYRPVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCEECCCCEECHHHC
NGKRPDFLFPSKAAYDNPAFPAERLRMLAAKTTCKDRWRQVLNEADRIATKHLLTLQEGV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEGQFREMSEAGVRLVVPAELHRSYPASVRPHLVTFENFIGDVRPLSAIQGP
CCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCEEEHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]