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The map label for this gene is pinE [H]

Identifier: 148244073

GI number: 148244073

Start: 21120

End: 21713

Strand: Reverse

Name: pinE [H]

Synonym: Acry_3570

Alternate gene names: 148244073

Gene position: 21713-21120 (Counterclockwise)

Preceding gene: 148244078

Following gene: 148244072

Centisome position: 58.03

GC content: 66.33

Gene sequence:

>594_bases
ATGCTGATCGGCTACATGCGCGTCTCGACCGACAATGACCGACAGGTGCTCGACCTGCAACGCGACGCCCTGCTCGGAGC
CGGGGTCGATGAGCGGCATCTGTTCGACGATCGTGCCGCGGGCAGCCGGCGCGACCGGCCGGGCCTCGCTGCGGCACTGG
CCTATCTGAAATCCGGCGATTGCCTCGTGGTCTGGAAGCTGGACCGGCTCGGCCGGTCGCTGCCGCATCTTCTGGAGACG
GTGAACAGCCTTAGGGCGCGTGGCGTCGGCTTCCGGTCGCTGACCGAGCAGATGGACACCACGACGCCGCAGGGGGAATT
CCTGTTTCATGTCTTTGGCGCTCTGGCGCAGTTCGAGCGTTCGCTGATCCAGGAGCGTGTGCAGGCAGGTCTGGCGGCCG
CCCGTCGGCGGGGCAGGCGTGGTGGCCGACCGATGGCGATCGATGCCGAGAAGCTGGCGGCGGTGATCGCGGCGCTGGAC
GCTGGTGCCACGAAAGCGTCGGTCTGCCGCACTTTCGGCATTTCGCGGAGTACGCTGGTCGACTCTCTGGCACGGATCGG
GCTAATGTCTGCGCTTGTGGTGGAAATTGTCTGA

Upstream 100 bases:

>100_bases
GACCTGCCGAAAAGTCCCAGATTTTGTGCCGAATCATGTACGGAAAACATAGTCGGAATGGCATGATATACGGAAACCGT
CTCGAAGGGGATTTCCGTAC

Downstream 100 bases:

>100_bases
CTGCCGTGCGTTGGATTGGCGCCGGAGGTCGGCGTAGCCGGCCGGAGGGGCCGATCCAACGCATGAGCCTGGGTTTAGGT
GGCCATCGGATCGAGCGGCT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLIGYMRVSTDNDRQVLDLQRDALLGAGVDERHLFDDRAAGSRRDRPGLAAALAYLKSGDCLVVWKLDRLGRSLPHLLET
VNSLRARGVGFRSLTEQMDTTTPQGEFLFHVFGALAQFERSLIQERVQAGLAAARRRGRRGGRPMAIDAEKLAAVIAALD
AGATKASVCRTFGISRSTLVDSLARIGLMSALVVEIV

Sequences:

>Translated_197_residues
MLIGYMRVSTDNDRQVLDLQRDALLGAGVDERHLFDDRAAGSRRDRPGLAAALAYLKSGDCLVVWKLDRLGRSLPHLLET
VNSLRARGVGFRSLTEQMDTTTPQGEFLFHVFGALAQFERSLIQERVQAGLAAARRRGRRGGRPMAIDAEKLAAVIAALD
AGATKASVCRTFGISRSTLVDSLARIGLMSALVVEIV
>Mature_197_residues
MLIGYMRVSTDNDRQVLDLQRDALLGAGVDERHLFDDRAAGSRRDRPGLAAALAYLKSGDCLVVWKLDRLGRSLPHLLET
VNSLRARGVGFRSLTEQMDTTTPQGEFLFHVFGALAQFERSLIQERVQAGLAAARRRGRRGGRPMAIDAEKLAAVIAALD
AGATKASVCRTFGISRSTLVDSLARIGLMSALVVEIV

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=179, Percent_Identity=50.2793296089386, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787827, Length=190, Percent_Identity=33.6842105263158, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787638, Length=190, Percent_Identity=33.1578947368421, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21418; Mature: 21418

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYMRVSTDNDRQVLDLQRDALLGAGVDERHLFDDRAAGSRRDRPGLAAALAYLKSGD
CEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
CLVVWKLDRLGRSLPHLLETVNSLRARGVGFRSLTEQMDTTTPQGEFLFHVFGALAQFER
EEEEEEHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SLIQERVQAGLAAARRRGRRGGRPMAIDAEKLAAVIAALDAGATKASVCRTFGISRSTLV
HHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
DSLARIGLMSALVVEIV
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIGYMRVSTDNDRQVLDLQRDALLGAGVDERHLFDDRAAGSRRDRPGLAAALAYLKSGD
CEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
CLVVWKLDRLGRSLPHLLETVNSLRARGVGFRSLTEQMDTTTPQGEFLFHVFGALAQFER
EEEEEEHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SLIQERVQAGLAAARRRGRRGGRPMAIDAEKLAAVIAALDAGATKASVCRTFGISRSTLV
HHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
DSLARIGLMSALVVEIV
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]