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The map label for this gene is prfA [H]

Identifier: 89256990

GI number: 89256990

Start: 1651704

End: 1652789

Strand: Reverse

Name: prfA [H]

Synonym: FTL_1721

Alternate gene names: 89256990

Gene position: 1652789-1651704 (Counterclockwise)

Preceding gene: 89256991

Following gene: 89256989

Centisome position: 87.17

GC content: 39.23

Gene sequence:

>1086_bases
ATGAAAGATTCTATTAAAGCAAAATTGCAAAGTTTAATAGAAAGGCATGAGGAAGTTAGTGCCTTATTAAGTGAGGCAGG
AATTATCTCTGATCAAAATAAGTTCAGAGATTTATCAAAAGAATACTCTCATCTTGAGCCGATTGTAAAGGCGTTTAAAG
AATATACACAAGCTTTAGAAGACAAACAAGCTGCTTATGAAATGCTTAATGAAAAAGATGCTGAGCTTGTAGAGATGGCA
AAAGAAGAATTAAAGTTGGCAAATGAAGCTATTGAGAAACTGGAGAGTGAATTACAAATATTTCTTTTACCTCGTGATCC
TAATGATGATGCTAACGTTTTCTTGGAGATTCGTGCAGGAACTGGCGGTGATGAGGCTTCGATATTTTCAGGTGATTTGT
TTAAGATGTATTCAAAATATGCTGAGCAAAGAGGCTGGAAGATTGAAGTAATATCGGCTAGTGAAGGCGAGCATGGCGGT
TATAAGGAAATCATCTCAAGAATATATGGCGATGGTGTATATTCACAACTAAAATTTGAATCTGGTGCTCACCGAGTACA
AAGAGTTCCTGCTACAGAGTCGCAGGGTAGAATCCATACTTCAGCGTGTACTGTTGCGGTTATGCCTGAAGCAGATGAGG
TTGAGGGGATTGATATTAATCCTGCAGATATCAAAGTGGATACTTTTAGAGCATCGGGTGCGGGAGGACAGCACGTCAAC
AAGACTGATTCAGCGATTAGGATTACCCATATCCCAACAGGTGTTGTTGTCGAATGCCAAGATCAAAGATCACAGCACAA
AAACCGTGCAGCTGCAATGTCGATGCTTAAATCAAAGCTGTTACAAGCAGAAATTGATAAGCAGCAAAAAGAGCAATCTG
ATACACGTAAGAGCCTGGTCGGTAGCGGTGATAGATCAGAGAGAATTAGAACTTATAATTACCCGCAAGGTAGAGTTACC
GATCATCGTATAAATTTAACTCTTTATAAGCTTGATGAGGTCATGGAAGGTAGTCTAGATAGTATTATCCAACCACTAGT
TCTAGAACATCAGGCGGATCTTTTAGCAACCATGTCTGATGAGTAA

Upstream 100 bases:

>100_bases
ACACTATCCTGTTGTAGGAATGAAAGAGGCTTCCAAGCAGGGTAGAAGTGACTGTCTTGTATGTATGAAACGTATGTTTG
GTTTAAATGTGGAAAAATAA

Downstream 100 bases:

>100_bases
TATTACAATTTCTCAATTATTAGCTTTAAGCTTAGCTAAATTTAATCAATGCGATATATCAACCAAACATGATCTACAAA
TGATTATTTGTGATGTTTTG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKDSIKAKLQSLIERHEEVSALLSEAGIISDQNKFRDLSKEYSHLEPIVKAFKEYTQALEDKQAAYEMLNEKDAELVEMA
KEELKLANEAIEKLESELQIFLLPRDPNDDANVFLEIRAGTGGDEASIFSGDLFKMYSKYAEQRGWKIEVISASEGEHGG
YKEIISRIYGDGVYSQLKFESGAHRVQRVPATESQGRIHTSACTVAVMPEADEVEGIDINPADIKVDTFRASGAGGQHVN
KTDSAIRITHIPTGVVVECQDQRSQHKNRAAAMSMLKSKLLQAEIDKQQKEQSDTRKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEVMEGSLDSIIQPLVLEHQADLLATMSDE

Sequences:

>Translated_361_residues
MKDSIKAKLQSLIERHEEVSALLSEAGIISDQNKFRDLSKEYSHLEPIVKAFKEYTQALEDKQAAYEMLNEKDAELVEMA
KEELKLANEAIEKLESELQIFLLPRDPNDDANVFLEIRAGTGGDEASIFSGDLFKMYSKYAEQRGWKIEVISASEGEHGG
YKEIISRIYGDGVYSQLKFESGAHRVQRVPATESQGRIHTSACTVAVMPEADEVEGIDINPADIKVDTFRASGAGGQHVN
KTDSAIRITHIPTGVVVECQDQRSQHKNRAAAMSMLKSKLLQAEIDKQQKEQSDTRKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEVMEGSLDSIIQPLVLEHQADLLATMSDE
>Mature_361_residues
MKDSIKAKLQSLIERHEEVSALLSEAGIISDQNKFRDLSKEYSHLEPIVKAFKEYTQALEDKQAAYEMLNEKDAELVEMA
KEELKLANEAIEKLESELQIFLLPRDPNDDANVFLEIRAGTGGDEASIFSGDLFKMYSKYAEQRGWKIEVISASEGEHGG
YKEIISRIYGDGVYSQLKFESGAHRVQRVPATESQGRIHTSACTVAVMPEADEVEGIDINPADIKVDTFRASGAGGQHVN
KTDSAIRITHIPTGVVVECQDQRSQHKNRAAAMSMLKSKLLQAEIDKQQKEQSDTRKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEVMEGSLDSIIQPLVLEHQADLLATMSDE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=301, Percent_Identity=43.8538205980066, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=40.9883720930233, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=41.2621359223301, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=65.0969529085873, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=40.4191616766467, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=270, Percent_Identity=38.1481481481481, Blast_Score=190, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=43.6363636363636, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19921226, Length=329, Percent_Identity=38.9057750759878, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40403; Mature: 40403

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSIKAKLQSLIERHEEVSALLSEAGIISDQNKFRDLSKEYSHLEPIVKAFKEYTQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKQAAYEMLNEKDAELVEMAKEELKLANEAIEKLESELQIFLLPRDPNDDANVFLEIRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECC
TGGDEASIFSGDLFKMYSKYAEQRGWKIEVISASEGEHGGYKEIISRIYGDGVYSQLKFE
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHH
SGAHRVQRVPATESQGRIHTSACTVAVMPEADEVEGIDINPADIKVDTFRASGAGGQHVN
HHHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGVVVECQDQRSQHKNRAAAMSMLKSKLLQAEIDKQQKEQSDTRKSLV
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNYPQGRVTDHRINLTLYKLDEVMEGSLDSIIQPLVLEHQADLLATMSD
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
E
C
>Mature Secondary Structure
MKDSIKAKLQSLIERHEEVSALLSEAGIISDQNKFRDLSKEYSHLEPIVKAFKEYTQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKQAAYEMLNEKDAELVEMAKEELKLANEAIEKLESELQIFLLPRDPNDDANVFLEIRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECC
TGGDEASIFSGDLFKMYSKYAEQRGWKIEVISASEGEHGGYKEIISRIYGDGVYSQLKFE
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHH
SGAHRVQRVPATESQGRIHTSACTVAVMPEADEVEGIDINPADIKVDTFRASGAGGQHVN
HHHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGVVVECQDQRSQHKNRAAAMSMLKSKLLQAEIDKQQKEQSDTRKSLV
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNYPQGRVTDHRINLTLYKLDEVMEGSLDSIIQPLVLEHQADLLATMSD
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA