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The map label for this gene is polA [H]

Identifier: 89256939

GI number: 89256939

Start: 1596731

End: 1599424

Strand: Reverse

Name: polA [H]

Synonym: FTL_1666

Alternate gene names: 89256939

Gene position: 1599424-1596731 (Counterclockwise)

Preceding gene: 89256940

Following gene: 89256938

Centisome position: 84.36

GC content: 35.12

Gene sequence:

>2694_bases
ATGAAAAAAATTGTTTTAGTGGATGGCTCTTCATATCTTTTTAGGGCTTATCATGCTTTACCACATCTTACTAATAGCCA
AGGTGAGCCTACGGGAGCAATCATTGGTGTTATCAATATGCTTAAAAAATTACCCATAATGTATGATACTGAGTATGTTG
CGGTTGTTTTTGATGCAAAGGGTAAGAACTTTCGTCATCAGTTATATCCACAATATAAAGCACATCGTAAAGATATCGAT
GATGAGCTAAGAGTCCAAATTCAACCATTACATCAGATTATTGAGAAGATGGGATTTGCTGTAATCATTGAAGATGGTGT
CGAGGCTGATGATGTAATAGGTACCTTAGCGCAAAAACTTCAAAAACAAGATTACCAAATTATTATTTCTACTGGCGACA
AAGATATGGCTCAGCTAGTTACAGATAACATAGTCTTATATGACTCAATGAAAAATGTCACTACAGATGTTGCTGGAGTG
CTAGAAAAATATCAAATTAGTCCACATCAGATGATAGATTATTTAGCCTTAATGGGAGATTCATCAGATAATATTCCAGG
TATTCCAAAAGTTGGTCCTAAAACAGCAGTTAAATGGTTACAAGACTATCAAAATATTGATGGTATAATCGCAAACCAGC
AGCAAATTAAGGGTAAGGTTGGTGAAAATCTACGTAACAATATTGATTTACTCAAGCTATCTTATCAGTTAGCAACAATT
AAATGTGATCTTGAGCTTGATTTGACTGTAGAGGATCTGAAATGCAAGGCTGCTGATAAAGCATATTTGATAGAAGCTTA
TACAAGATATGAGTTTAAATCATTATTGAAAGACTTAGATAATAGCGATATTAAGATAGTAACAAGCCAAGCACAAGCTG
CTGATGTTGCTATTGAATATATTACTATTACCACACAAACTCAGCTTGATGATCTGATTGCGGAACTAGAGAAATATGAT
AGCTTTGCTTTTGACACGGAAACGGATTCTTTAAATACTTATGAAGCAAACCTAGTGGGCTTATCTTTTTGTGCAAAAGA
GGGACGAGCTTTTTATATCCCATTACAACATAGATACTTAGGCGTGCCACAGCAACTAGAATTAGGATTTGTCTTAGATA
AGCTAAAACCATTATTTGCAGACTCTAAGAAAGTGAAAGTTGCACATAACTTCAAATTTGATGAAAAAGTTCTATCAAAA
TATGCGATTGAGATAAACGGTAAAGTCGATGATACGATGATTATGGCGTATGTACTAAAAAGTAGTGGTAAGCATGATAT
GGATAGTCTTTCTAAAGAGCATCTTGGCATAGAACCAATTGCTTATACTGCAATAGCTGGTACTGGTAAACAGCAACAAA
CTCTGGATCAGGTAGATATCGAGATAGTTGCTAAGTACGCTGCTGAAGATGCTGATATTACTTTTAGACTTTTTAATCAC
TTTAAAGCTTTATTAGAGCAAGATAAGGTTCTTTTTAAACTTTATTGTGAACTCGAAATGCCTTTGACTATCATTCTTAA
TCAGATGGAGAAAACAGGTGTTAAGATAGATGCTACTAAGCTTATACAGCAAAGTGCTAGTCTTGAAACATCTATCAAAG
AGCTTGAAAGCAAATGTTATAACCTCGCTGGGCAAGAATTTAACCTATCTTCACCAGTACAGTTAAGAGAAATTCTTTTC
GAGAAGCTAGGACTTCCTCCAGTTAAAAAAACAGCAAAGGGTCAAGTGTCGACTTCTGAAGAGGTGCTAGTTCAATTGGC
TGAGGACTATGAAATCGCAGCATTGATAATGAAATATCGCCATTTATCAAAACTTAAAAATACATATACAGATAAGCTAC
CTAAAATGTTGGACGCAAATGGTCGTGTACATACCTCATATAATCAAACTGGTACCGTAACAGGTAGGCTATCCTCATCG
GATCCTAATTTACAAAATATTCCTATAAAAAGTCCTGAAGGGCGTAAAATTAGGGAAGCTTTTATAGCTGAGGATGGTTA
CTGTATAGTTGCTGCAGATTATTCACAAATTGAACTTAGAATCATGGCACATTTATCAAAAGATAAAAACCTTCTCAAGG
TGTTTAATCAAAACTTAGATATCCATAGTGCAACAGCTGCTGAAGTTTTAGGTATTAGTATTGATGAGGTTAGTAGTGAG
CAAAGAAGAAAAGCTAAAGCAATAAACTTCGGTCTTATCTATGGTATGAGTGCTTTTGGTTTAGCTAGACAGTTAGAAAT
ACCTCGAGCTGAAGCGCAAGAATATATTGATATCTATTTTAATCGCTATCCAAGTGTCAAAGAGTACATGACTACAGCAA
AAGAGTTTGCTAAGCAAAATGGCTATGTTGAAACTATATTAGGAAGAAGATTATATTTACCAGAGATTAACTCAAAAAAT
GTTATGCAGCGTAATGCAGCAGAGCGAGCAGCGATTAACGCACCAATGCAGGGCACCGCTGCTGATATTATCAAAAAAGC
AATGATAAATGTTAACATGATGATCTCACAGGAGTATAATAGCGAAATTAAGATGGTGATGCAGGTTCATGATGAGCTTG
TTTTTGAAGTTAAAAAAACTAAGCTTGAAGAGATAGTTGCGAAGATAAAATCTATAATGGAAGCAGCTGTAAAGCTTAAT
GTGCCTTTAGAGGTGAATGTTGATTCTGGTAAGAGCTGGGATCAAGCTCATTAA

Upstream 100 bases:

>100_bases
TTTAAAAATCTCATAAAACAACAATGTTACAACAAATGAATTTGCTATACTAAAGTATATATATTACTATTAAAATGATA
AAAAAATTGCAATAAAATCT

Downstream 100 bases:

>100_bases
ATAATTTATAGGATATTTTATGTTATTAGTAATGGATATGGGCAATTCCCATATACACATTGGTGTGTTTGATGGTGATA
TAATAGTTTCTCAAATTAGA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 897; Mature: 897

Protein sequence:

>897_residues
MKKIVLVDGSSYLFRAYHALPHLTNSQGEPTGAIIGVINMLKKLPIMYDTEYVAVVFDAKGKNFRHQLYPQYKAHRKDID
DELRVQIQPLHQIIEKMGFAVIIEDGVEADDVIGTLAQKLQKQDYQIIISTGDKDMAQLVTDNIVLYDSMKNVTTDVAGV
LEKYQISPHQMIDYLALMGDSSDNIPGIPKVGPKTAVKWLQDYQNIDGIIANQQQIKGKVGENLRNNIDLLKLSYQLATI
KCDLELDLTVEDLKCKAADKAYLIEAYTRYEFKSLLKDLDNSDIKIVTSQAQAADVAIEYITITTQTQLDDLIAELEKYD
SFAFDTETDSLNTYEANLVGLSFCAKEGRAFYIPLQHRYLGVPQQLELGFVLDKLKPLFADSKKVKVAHNFKFDEKVLSK
YAIEINGKVDDTMIMAYVLKSSGKHDMDSLSKEHLGIEPIAYTAIAGTGKQQQTLDQVDIEIVAKYAAEDADITFRLFNH
FKALLEQDKVLFKLYCELEMPLTIILNQMEKTGVKIDATKLIQQSASLETSIKELESKCYNLAGQEFNLSSPVQLREILF
EKLGLPPVKKTAKGQVSTSEEVLVQLAEDYEIAALIMKYRHLSKLKNTYTDKLPKMLDANGRVHTSYNQTGTVTGRLSSS
DPNLQNIPIKSPEGRKIREAFIAEDGYCIVAADYSQIELRIMAHLSKDKNLLKVFNQNLDIHSATAAEVLGISIDEVSSE
QRRKAKAINFGLIYGMSAFGLARQLEIPRAEAQEYIDIYFNRYPSVKEYMTTAKEFAKQNGYVETILGRRLYLPEINSKN
VMQRNAAERAAINAPMQGTAADIIKKAMINVNMMISQEYNSEIKMVMQVHDELVFEVKKTKLEEIVAKIKSIMEAAVKLN
VPLEVNVDSGKSWDQAH

Sequences:

>Translated_897_residues
MKKIVLVDGSSYLFRAYHALPHLTNSQGEPTGAIIGVINMLKKLPIMYDTEYVAVVFDAKGKNFRHQLYPQYKAHRKDID
DELRVQIQPLHQIIEKMGFAVIIEDGVEADDVIGTLAQKLQKQDYQIIISTGDKDMAQLVTDNIVLYDSMKNVTTDVAGV
LEKYQISPHQMIDYLALMGDSSDNIPGIPKVGPKTAVKWLQDYQNIDGIIANQQQIKGKVGENLRNNIDLLKLSYQLATI
KCDLELDLTVEDLKCKAADKAYLIEAYTRYEFKSLLKDLDNSDIKIVTSQAQAADVAIEYITITTQTQLDDLIAELEKYD
SFAFDTETDSLNTYEANLVGLSFCAKEGRAFYIPLQHRYLGVPQQLELGFVLDKLKPLFADSKKVKVAHNFKFDEKVLSK
YAIEINGKVDDTMIMAYVLKSSGKHDMDSLSKEHLGIEPIAYTAIAGTGKQQQTLDQVDIEIVAKYAAEDADITFRLFNH
FKALLEQDKVLFKLYCELEMPLTIILNQMEKTGVKIDATKLIQQSASLETSIKELESKCYNLAGQEFNLSSPVQLREILF
EKLGLPPVKKTAKGQVSTSEEVLVQLAEDYEIAALIMKYRHLSKLKNTYTDKLPKMLDANGRVHTSYNQTGTVTGRLSSS
DPNLQNIPIKSPEGRKIREAFIAEDGYCIVAADYSQIELRIMAHLSKDKNLLKVFNQNLDIHSATAAEVLGISIDEVSSE
QRRKAKAINFGLIYGMSAFGLARQLEIPRAEAQEYIDIYFNRYPSVKEYMTTAKEFAKQNGYVETILGRRLYLPEINSKN
VMQRNAAERAAINAPMQGTAADIIKKAMINVNMMISQEYNSEIKMVMQVHDELVFEVKKTKLEEIVAKIKSIMEAAVKLN
VPLEVNVDSGKSWDQAH
>Mature_897_residues
MKKIVLVDGSSYLFRAYHALPHLTNSQGEPTGAIIGVINMLKKLPIMYDTEYVAVVFDAKGKNFRHQLYPQYKAHRKDID
DELRVQIQPLHQIIEKMGFAVIIEDGVEADDVIGTLAQKLQKQDYQIIISTGDKDMAQLVTDNIVLYDSMKNVTTDVAGV
LEKYQISPHQMIDYLALMGDSSDNIPGIPKVGPKTAVKWLQDYQNIDGIIANQQQIKGKVGENLRNNIDLLKLSYQLATI
KCDLELDLTVEDLKCKAADKAYLIEAYTRYEFKSLLKDLDNSDIKIVTSQAQAADVAIEYITITTQTQLDDLIAELEKYD
SFAFDTETDSLNTYEANLVGLSFCAKEGRAFYIPLQHRYLGVPQQLELGFVLDKLKPLFADSKKVKVAHNFKFDEKVLSK
YAIEINGKVDDTMIMAYVLKSSGKHDMDSLSKEHLGIEPIAYTAIAGTGKQQQTLDQVDIEIVAKYAAEDADITFRLFNH
FKALLEQDKVLFKLYCELEMPLTIILNQMEKTGVKIDATKLIQQSASLETSIKELESKCYNLAGQEFNLSSPVQLREILF
EKLGLPPVKKTAKGQVSTSEEVLVQLAEDYEIAALIMKYRHLSKLKNTYTDKLPKMLDANGRVHTSYNQTGTVTGRLSSS
DPNLQNIPIKSPEGRKIREAFIAEDGYCIVAADYSQIELRIMAHLSKDKNLLKVFNQNLDIHSATAAEVLGISIDEVSSE
QRRKAKAINFGLIYGMSAFGLARQLEIPRAEAQEYIDIYFNRYPSVKEYMTTAKEFAKQNGYVETILGRRLYLPEINSKN
VMQRNAAERAAINAPMQGTAADIIKKAMINVNMMISQEYNSEIKMVMQVHDELVFEVKKTKLEEIVAKIKSIMEAAVKLN
VPLEVNVDSGKSWDQAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=465, Percent_Identity=33.9784946236559, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI139394648, Length=706, Percent_Identity=27.9036827195467, Blast_Score=228, Evalue=2e-59,
Organism=Escherichia coli, GI1790294, Length=921, Percent_Identity=52.3344191096634, Blast_Score=918, Evalue=0.0,
Organism=Escherichia coli, GI226510970, Length=253, Percent_Identity=28.0632411067194, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=370, Percent_Identity=31.0810810810811, Blast_Score=172, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17933644, Length=581, Percent_Identity=31.4974182444062, Blast_Score=246, Evalue=8e-65,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 101003; Mature: 101003

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVLVDGSSYLFRAYHALPHLTNSQGEPTGAIIGVINMLKKLPIMYDTEYVAVVFDAK
CCEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEEEEECC
GKNFRHQLYPQYKAHRKDIDDELRVQIQPLHQIIEKMGFAVIIEDGVEADDVIGTLAQKL
CCCCHHHCCCHHHHHHCCCCHHHHEEEHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
QKQDYQIIISTGDKDMAQLVTDNIVLYDSMKNVTTDVAGVLEKYQISPHQMIDYLALMGD
CCCCCEEEEECCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
SSDNIPGIPKVGPKTAVKWLQDYQNIDGIIANQQQIKGKVGENLRNNIDLLKLSYQLATI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEEEEEEEEEE
KCDLELDLTVEDLKCKAADKAYLIEAYTRYEFKSLLKDLDNSDIKIVTSQAQAADVAIEY
EEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
ITITTQTQLDDLIAELEKYDSFAFDTETDSLNTYEANLVGLSFCAKEGRAFYIPLQHRYL
EEEECHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHEEEHHHHHCCCCEEEEEEHHHHC
GVPQQLELGFVLDKLKPLFADSKKVKVAHNFKFDEKVLSKYAIEINGKVDDTMIMAYVLK
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEEEEECCCCCHHHHHHHHHH
SSGKHDMDSLSKEHLGIEPIAYTAIAGTGKQQQTLDQVDIEIVAKYAAEDADITFRLFNH
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FKALLEQDKVLFKLYCELEMPLTIILNQMEKTGVKIDATKLIQQSASLETSIKELESKCY
HHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
NLAGQEFNLSSPVQLREILFEKLGLPPVKKTAKGQVSTSEEVLVQLAEDYEIAALIMKYR
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
HLSKLKNTYTDKLPKMLDANGRVHTSYNQTGTVTGRLSSSDPNLQNIPIKSPEGRKIREA
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
FIAEDGYCIVAADYSQIELRIMAHLSKDKNLLKVFNQNLDIHSATAAEVLGISIDEVSSE
HHCCCCEEEEEECCCCEEEHEEHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCHHHHCHH
QRRKAKAINFGLIYGMSAFGLARQLEIPRAEAQEYIDIYFNRYPSVKEYMTTAKEFAKQN
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GYVETILGRRLYLPEINSKNVMQRNAAERAAINAPMQGTAADIIKKAMINVNMMISQEYN
CCEEHHHCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEECCCC
SEIKMVMQVHDELVFEVKKTKLEEIVAKIKSIMEAAVKLNVPLEVNVDSGKSWDQAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKIVLVDGSSYLFRAYHALPHLTNSQGEPTGAIIGVINMLKKLPIMYDTEYVAVVFDAK
CCEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEEEEECC
GKNFRHQLYPQYKAHRKDIDDELRVQIQPLHQIIEKMGFAVIIEDGVEADDVIGTLAQKL
CCCCHHHCCCHHHHHHCCCCHHHHEEEHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
QKQDYQIIISTGDKDMAQLVTDNIVLYDSMKNVTTDVAGVLEKYQISPHQMIDYLALMGD
CCCCCEEEEECCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
SSDNIPGIPKVGPKTAVKWLQDYQNIDGIIANQQQIKGKVGENLRNNIDLLKLSYQLATI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEEEEEEEEEE
KCDLELDLTVEDLKCKAADKAYLIEAYTRYEFKSLLKDLDNSDIKIVTSQAQAADVAIEY
EEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
ITITTQTQLDDLIAELEKYDSFAFDTETDSLNTYEANLVGLSFCAKEGRAFYIPLQHRYL
EEEECHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHEEEHHHHHCCCCEEEEEEHHHHC
GVPQQLELGFVLDKLKPLFADSKKVKVAHNFKFDEKVLSKYAIEINGKVDDTMIMAYVLK
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEEEEECCCCCHHHHHHHHHH
SSGKHDMDSLSKEHLGIEPIAYTAIAGTGKQQQTLDQVDIEIVAKYAAEDADITFRLFNH
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FKALLEQDKVLFKLYCELEMPLTIILNQMEKTGVKIDATKLIQQSASLETSIKELESKCY
HHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
NLAGQEFNLSSPVQLREILFEKLGLPPVKKTAKGQVSTSEEVLVQLAEDYEIAALIMKYR
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
HLSKLKNTYTDKLPKMLDANGRVHTSYNQTGTVTGRLSSSDPNLQNIPIKSPEGRKIREA
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
FIAEDGYCIVAADYSQIELRIMAHLSKDKNLLKVFNQNLDIHSATAAEVLGISIDEVSSE
HHCCCCEEEEEECCCCEEEHEEHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCHHHHCHH
QRRKAKAINFGLIYGMSAFGLARQLEIPRAEAQEYIDIYFNRYPSVKEYMTTAKEFAKQN
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GYVETILGRRLYLPEINSKNVMQRNAAERAAINAPMQGTAADIIKKAMINVNMMISQEYN
CCEEHHHCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEECCCC
SEIKMVMQVHDELVFEVKKTKLEEIVAKIKSIMEAAVKLNVPLEVNVDSGKSWDQAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]