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The map label for this gene is prfC [H]

Identifier: 89256932

GI number: 89256932

Start: 1589283

End: 1590860

Strand: Reverse

Name: prfC [H]

Synonym: FTL_1659

Alternate gene names: 89256932

Gene position: 1590860-1589283 (Counterclockwise)

Preceding gene: 89256933

Following gene: 89256931

Centisome position: 83.91

GC content: 36.5

Gene sequence:

>1578_bases
ATGAGTGAATATCTACAACAAATCGCTAAGCGTAGGACTTTTGCAATTATTTCTCACCCAGATGCTGGTAAGACAACAAT
TACAGAAAAAATGTTACTTTTTGGTAATGCTATCAAGACAGCAGGAACTGTTAAAGCTAAAAAAAGTGGTATACATGCTA
CATCGGATTGGATGGAGATGGAGAAACAAAGAGGAATTTCAATCACAACCTCGGTAATGCAATTTCCCTATAATGGACGT
ATTATAAATCTACTTGATACTCCAGGACATGAAGATTTCTCTGAGGATACTTATAGAACACTTACTGCGGTAGATTCAGC
ACTAATGGTTGTTGATGCAGTTAAAGGTGTCGAGGATAGGACTATTAAGCTGATGAATGTTTGTCGCTTAAGAGATACGC
CTATTGTAACTTTTATGAATAAGTTTGATCGAGATACTCGTGATCCTCTTGAGCTTTTAGATGAAGTTGAGAATATCCTT
AAGATAAAATGTGCTCCAATGAATTGGCCAATTGGTATGGGTAAATATTTTAAAGGAGTTTACGATTTGTATAATGATGA
AGTTACTCTATTTGAAACAGGTCATGGGCACGAGATATATCCATACAAAAAGATCAAAGGTCTAGCAAATGCTAAAGATT
CTATAGGCATAGACCTCTATGAAGATCTTGAGATGGAAATTGATTTAGTGCGTGGCGCAAGTCATGAGTTTGATGAGCAG
GAATTTCTAGAGGGCAATCTCACTCCAGTATATTTTGGTACTGCACTGAGTAATTTTGGTGTCAAAGAAATGATGGATGG
TTTTACAAGGTACGCTCCAGCACCCCAACATCGCGAAGCTGATCAAAGAGTTGTTGCTGCAGACGAGCAAAAATTGACAG
GTTTTGTATTTAAAATACAAGCAAATATGGATGAAAAACATCGCAATAGAATTGCATTTTTTAGAATTTGTTCTGGCAAG
TATGAAAAAGGTATGAAAATTTTTCATGAAAGAACCGGTAAGCAAATGCAAATCTCAAAAGCACTAACTTTCATGGCAGG
TGAAAGAGAGCAAGTTGAAGAAGGTTATGCTGGGGATATTATTGGTTTACATAATCATGGTAGTATTCAGATTGGCGATA
GTTTTACTCAAGGAGAAAAACTAAAATTCAAAGGCATACCAAATTTTGCTCCTGAGATTTTCAAAAGAGTTAAACTAAAT
GATCCACTAAAAATGAAAGCATTACAAAAAGGTTTAGTGCAGCTATCAGAAGAAGGCGCTACACAAGTTTTCAAACCTTT
TATATCGAATGATTTGGTACTTGGAGCTGTTGGTGTTTTGCAGTTTGATGTGGTTGCACAGCGTTTGGCATCAGAGTACA
ATGTCAAATGTTCGTACGAGGGAGTGAACGTTACCTTAGCACGTTGGATTTTTTGTAATGATGAGAAAAAGCTTAATGAT
TTTAAGAAAAAGTATGAGGTGAATTTGGCTTATGATGGGGCAGGGTATCTTACATATCTAGCGCCAACAGGGGTTAATTT
ACAGCTGGCACAAGAAAAAAATCCAGATATCATATTTAGTGCAACAAGAGAGCACTAG

Upstream 100 bases:

>100_bases
TATCTAATATTAGATAAACATTTTAATTTTCAAAATTAATCTATTTCTAGTAAACTTCTCTTCATTAAATATTATCTTCA
AATAGTTAGAAAGAATTATT

Downstream 100 bases:

>100_bases
TATCATATACTTTTTAACTAGAAAAAATATGCTATAATTTTTTAGTCTTTGTTTATAATTTGTTAGGGTGCTAAATGTTA
AAAAGTATCAGTAGGGTTCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSEYLQQIAKRRTFAIISHPDAGKTTITEKMLLFGNAIKTAGTVKAKKSGIHATSDWMEMEKQRGISITTSVMQFPYNGR
IINLLDTPGHEDFSEDTYRTLTAVDSALMVVDAVKGVEDRTIKLMNVCRLRDTPIVTFMNKFDRDTRDPLELLDEVENIL
KIKCAPMNWPIGMGKYFKGVYDLYNDEVTLFETGHGHEIYPYKKIKGLANAKDSIGIDLYEDLEMEIDLVRGASHEFDEQ
EFLEGNLTPVYFGTALSNFGVKEMMDGFTRYAPAPQHREADQRVVAADEQKLTGFVFKIQANMDEKHRNRIAFFRICSGK
YEKGMKIFHERTGKQMQISKALTFMAGEREQVEEGYAGDIIGLHNHGSIQIGDSFTQGEKLKFKGIPNFAPEIFKRVKLN
DPLKMKALQKGLVQLSEEGATQVFKPFISNDLVLGAVGVLQFDVVAQRLASEYNVKCSYEGVNVTLARWIFCNDEKKLND
FKKKYEVNLAYDGAGYLTYLAPTGVNLQLAQEKNPDIIFSATREH

Sequences:

>Translated_525_residues
MSEYLQQIAKRRTFAIISHPDAGKTTITEKMLLFGNAIKTAGTVKAKKSGIHATSDWMEMEKQRGISITTSVMQFPYNGR
IINLLDTPGHEDFSEDTYRTLTAVDSALMVVDAVKGVEDRTIKLMNVCRLRDTPIVTFMNKFDRDTRDPLELLDEVENIL
KIKCAPMNWPIGMGKYFKGVYDLYNDEVTLFETGHGHEIYPYKKIKGLANAKDSIGIDLYEDLEMEIDLVRGASHEFDEQ
EFLEGNLTPVYFGTALSNFGVKEMMDGFTRYAPAPQHREADQRVVAADEQKLTGFVFKIQANMDEKHRNRIAFFRICSGK
YEKGMKIFHERTGKQMQISKALTFMAGEREQVEEGYAGDIIGLHNHGSIQIGDSFTQGEKLKFKGIPNFAPEIFKRVKLN
DPLKMKALQKGLVQLSEEGATQVFKPFISNDLVLGAVGVLQFDVVAQRLASEYNVKCSYEGVNVTLARWIFCNDEKKLND
FKKKYEVNLAYDGAGYLTYLAPTGVNLQLAQEKNPDIIFSATREH
>Mature_524_residues
SEYLQQIAKRRTFAIISHPDAGKTTITEKMLLFGNAIKTAGTVKAKKSGIHATSDWMEMEKQRGISITTSVMQFPYNGRI
INLLDTPGHEDFSEDTYRTLTAVDSALMVVDAVKGVEDRTIKLMNVCRLRDTPIVTFMNKFDRDTRDPLELLDEVENILK
IKCAPMNWPIGMGKYFKGVYDLYNDEVTLFETGHGHEIYPYKKIKGLANAKDSIGIDLYEDLEMEIDLVRGASHEFDEQE
FLEGNLTPVYFGTALSNFGVKEMMDGFTRYAPAPQHREADQRVVAADEQKLTGFVFKIQANMDEKHRNRIAFFRICSGKY
EKGMKIFHERTGKQMQISKALTFMAGEREQVEEGYAGDIIGLHNHGSIQIGDSFTQGEKLKFKGIPNFAPEIFKRVKLND
PLKMKALQKGLVQLSEEGATQVFKPFISNDLVLGAVGVLQFDVVAQRLASEYNVKCSYEGVNVTLARWIFCNDEKKLNDF
KKKYEVNLAYDGAGYLTYLAPTGVNLQLAQEKNPDIIFSATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=28.5714285714286, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI19923640, Length=506, Percent_Identity=27.2727272727273, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI25306283, Length=376, Percent_Identity=30.0531914893617, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=31.9727891156463, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966754, Length=169, Percent_Identity=29.585798816568, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=67.741935483871, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=26.369168356998, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994988, Length=208, Percent_Identity=29.3269230769231, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=509, Percent_Identity=28.0943025540275, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=29.8342541436464, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=28.9473684210526, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=29.3233082706767, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=29.3233082706767, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=28.0922431865828, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6322359, Length=589, Percent_Identity=25.6366723259762, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=28.75, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=28.75, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=26.1437908496732, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582462, Length=495, Percent_Identity=29.0909090909091, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI221458488, Length=500, Percent_Identity=26.8, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=29.559748427673, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59417; Mature: 59286

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLQQIAKRRTFAIISHPDAGKTTITEKMLLFGNAIKTAGTVKAKKSGIHATSDWMEM
CHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCHHHCCCCCCHHHHHH
EKQRGISITTSVMQFPYNGRIINLLDTPGHEDFSEDTYRTLTAVDSALMVVDAVKGVEDR
HHHCCCEEEEHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TIKLMNVCRLRDTPIVTFMNKFDRDTRDPLELLDEVENILKIKCAPMNWPIGMGKYFKGV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHH
YDLYNDEVTLFETGHGHEIYPYKKIKGLANAKDSIGIDLYEDLEMEIDLVRGASHEFDEQ
HHHHCCCEEEEECCCCCEECCHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCH
EFLEGNLTPVYFGTALSNFGVKEMMDGFTRYAPAPQHREADQRVVAADEQKLTGFVFKIQ
HHHCCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
ANMDEKHRNRIAFFRICSGKYEKGMKIFHERTGKQMQISKALTFMAGEREQVEEGYAGDI
CCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHCCCCCCE
IGLHNHGSIQIGDSFTQGEKLKFKGIPNFAPEIFKRVKLNDPLKMKALQKGLVQLSEEGA
EEECCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCH
TQVFKPFISNDLVLGAVGVLQFDVVAQRLASEYNVKCSYEGVNVTLARWIFCNDEKKLND
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCHHHHH
FKKKYEVNLAYDGAGYLTYLAPTGVNLQLAQEKNPDIIFSATREH
HHHHEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEECCCC
>Mature Secondary Structure 
SEYLQQIAKRRTFAIISHPDAGKTTITEKMLLFGNAIKTAGTVKAKKSGIHATSDWMEM
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCHHHCCCCCCHHHHHH
EKQRGISITTSVMQFPYNGRIINLLDTPGHEDFSEDTYRTLTAVDSALMVVDAVKGVEDR
HHHCCCEEEEHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TIKLMNVCRLRDTPIVTFMNKFDRDTRDPLELLDEVENILKIKCAPMNWPIGMGKYFKGV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHH
YDLYNDEVTLFETGHGHEIYPYKKIKGLANAKDSIGIDLYEDLEMEIDLVRGASHEFDEQ
HHHHCCCEEEEECCCCCEECCHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCH
EFLEGNLTPVYFGTALSNFGVKEMMDGFTRYAPAPQHREADQRVVAADEQKLTGFVFKIQ
HHHCCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
ANMDEKHRNRIAFFRICSGKYEKGMKIFHERTGKQMQISKALTFMAGEREQVEEGYAGDI
CCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHCCCCCCE
IGLHNHGSIQIGDSFTQGEKLKFKGIPNFAPEIFKRVKLNDPLKMKALQKGLVQLSEEGA
EEECCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCH
TQVFKPFISNDLVLGAVGVLQFDVVAQRLASEYNVKCSYEGVNVTLARWIFCNDEKKLND
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCHHHHH
FKKKYEVNLAYDGAGYLTYLAPTGVNLQLAQEKNPDIIFSATREH
HHHHEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA