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The map label for this gene is glpK [H]

Identifier: 89256921

GI number: 89256921

Start: 1574234

End: 1575742

Strand: Reverse

Name: glpK [H]

Synonym: FTL_1644

Alternate gene names: 89256921

Gene position: 1575742-1574234 (Counterclockwise)

Preceding gene: 89256922

Following gene: 89256919

Centisome position: 83.11

GC content: 37.71

Gene sequence:

>1509_bases
ATGTCAAAAGATTTCATCTTAGCTATAGATCAAGGTACAACAAGTTCACGAGCAATTATCTTTGATAAAAAGGGTAATAT
AAGAAAGATTGCTCAAAAAGAATTCACACAAATATATCCTAAAAGTGGTTGGGTAGAACATGATGCAATGGAAATATGGG
GTACTCAAAGTGGCGTAATGCGTGAGGCACTAGAGTTTGGCCGCGTTAAGCCAGATCAAATTGCAGCAATAGGTATTACA
AATCAGCGCGAGACAGTTGTAGTTTGGGATAAAGAAACAGGTGATCCTGTCTATAATGCTATAGTGTGGCAATGTCGCCG
TACTTCAAGTATTTGTGATGAAATCAAACGAGATCCTCAATTTGTCAAATATATCAAAGAAAATACTGGTCTGGTTGTAG
ATGCGTATTTTTCAGGCACTAAAGTAAAATGGATTTTAGATAACGTTGAAGGTGCTAGAGAAAAAGCAAATGCCGGCAAA
TTATTAATGGGTACTATAGATACTTGGTTGATTTGGAATCTAACTCGCGGTAAAGTGCATGCGACAGACTATAGTAATGC
TTCGCGCACTATGTTATTTAATATTAATAGTTTAGAGTGGGATAAAAAAATACTTGATTACTTAAATATTCCTGAATCAA
TGTTACCTGAGGTTAAAAACTCAAGTGAGGTTTTTGGAGTTACAGATAGCCATACTTTAGGTGGTGCTGAGATTCCTATT
GCTGGTGTTGCAGGAGATCAGCATGCAGCATTATTTGGTCATTGCTGTTTTGAAAAAGGTATGGCAAAAAATACTTATGG
TACGGGTTGTTTTGCGTTGATGAATGTCGGCGATAAGCCAGTTTATTCAGATGAAGGTTTATTGACCACTATTGCATGGG
CAGAAAATGGTAAGCCAACTTATGCTTTAGAAGGTAGTATATTTATAGTAGGAGCTGTTATTCAGTGGATTCGTGATGGT
TTGGGACTAGTGCGTTCTGCTGAGGATAGTGAGTACTATGCTACTAAGATTGATTCTACAAATGGTGTATATTTGGTGCC
TGCGTTTGTTGGTCTTGGCACACCATATTGGGATATGTATGCTCGTGGTACGATAGTTGGCATTACCCGTGATACAAAGC
GTGAGCATATTATTCGTGCAGCTCTAGAGGCTATAGCATATCAAGCTAAAGATGTCTTAGAATGTATGAAAGAGGATACG
GGACTTGATCTTGCTGGTTTGCGTGTTGATGGTGGCGCGGTACAAAATAACTTCCTAATGCAGTTTCAATCTGATATTTT
ACAATCTGAAATCTCAAAACCGAAAATAAATGAAATTACTAGTTTGGGGGCTGTCTTCTTAGCTGGTTTAGCAGTTGGTT
TTTGGAAGGATAAACAAGAATTGAAATCTATTCTTACAACTGAAAAAGTTTTTGAACCGCAAAAAGACTCTCAAGCTGTT
GCTCATGATTATAGAGGTTGGAAGAAAGCAGTAGAAAGAAGTAAGGCTTGGGCAGAGTGTTATAGTTAA

Upstream 100 bases:

>100_bases
TATCAATAGTATTATCTCTTTTTATTACGTAGAAAAAAAACTAAAATATCCTTTAACAATATACAATTTGTATTAATCTT
TACAAAAAGGAATATCTATT

Downstream 100 bases:

>100_bases
TCCGAGAGTATTTTGTTAAACACATAAGAGATAGCATCACAAAATTTTTCAAAACTATTATTCACTTTTCTAAATATTTT
TTTAAAGTTAGCCCAAACCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSKDFILAIDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGIT
NQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGK
LLMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSIFIVGAVIQWIRDG
LGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDT
GLDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITSLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
AHDYRGWKKAVERSKAWAECYS

Sequences:

>Translated_502_residues
MSKDFILAIDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGIT
NQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGK
LLMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSIFIVGAVIQWIRDG
LGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDT
GLDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITSLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
AHDYRGWKKAVERSKAWAECYS
>Mature_501_residues
SKDFILAIDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGITN
QRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKL
LMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPIA
GVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSIFIVGAVIQWIRDGL
GLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTG
LDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITSLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAVA
HDYRGWKKAVERSKAWAECYS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=46.2151394422311, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=46.2151394422311, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=46.2450592885375, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.0199203187251, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=34.0551181102362, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=61.7706237424547, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=521, Percent_Identity=23.8003838771593, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790005, Length=488, Percent_Identity=23.155737704918, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=40.990099009901, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=25.3846153846154, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=297, Percent_Identity=28.6195286195286, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=558, Percent_Identity=32.9749103942652, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI17864214, Length=514, Percent_Identity=42.4124513618677, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655555, Length=504, Percent_Identity=37.5, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=504, Percent_Identity=37.5, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=504, Percent_Identity=37.5, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=504, Percent_Identity=37.5, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=504, Percent_Identity=37.5, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=440, Percent_Identity=43.4090909090909, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=31.6888045540797, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=31.6430020283976, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24656367, Length=520, Percent_Identity=29.2307692307692, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI21357867, Length=535, Percent_Identity=27.8504672897196, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=31.1428571428571, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=29.6551724137931, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55818; Mature: 55687

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDFILAIDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
REALEFGRVKPDQIAAIGITNQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQ
HHHHHHCCCCCHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKLLMGTIDTWLIWNLTRGKVH
HHHHHHHCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEECCEEEEEEECCCEEE
ATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
ECCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCCCCCCCEE
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPT
EECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCE
YALEGSIFIVGAVIQWIRDGLGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMY
EEECCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCHHHHH
ARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTGLDLAGLRVDGGAVQNNFLM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
QFQSDILQSEISKPKINEITSLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
AHDYRGWKKAVERSKAWAECYS
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDFILAIDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVM
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
REALEFGRVKPDQIAAIGITNQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQ
HHHHHHCCCCCHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKLLMGTIDTWLIWNLTRGKVH
HHHHHHHCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEECCEEEEEEECCCEEE
ATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
ECCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCCCCCCCEE
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPT
EECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCE
YALEGSIFIVGAVIQWIRDGLGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMY
EEECCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCHHHHH
ARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTGLDLAGLRVDGGAVQNNFLM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
QFQSDILQSEISKPKINEITSLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
AHDYRGWKKAVERSKAWAECYS
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA