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The map label for this gene is pckA [H]

Identifier: 89256893

GI number: 89256893

Start: 1544656

End: 1546242

Strand: Reverse

Name: pckA [H]

Synonym: FTL_1616

Alternate gene names: 89256893

Gene position: 1546242-1544656 (Counterclockwise)

Preceding gene: 89256897

Following gene: 89256892

Centisome position: 81.55

GC content: 32.51

Gene sequence:

>1587_bases
ATGGATCCTTCAACCATAGATGAGTATAAGTATCTATATACTTGTGAAAATGTGCATAAAAATCTCTCTCTCCAAGAATT
AGCAAAATACGCACATAATAGTGATGGTTTTATTTGCTACGATAATAAAACTTTAGTAGTTGGCAGTGGTGAAATCAAGG
GTCGACTTCCTGATGATAAATATATTGTTGAGACTAAATATGCCAAAAAAAATATTTGGTGGAGTGAAAATGGCTCAGAT
AATAAAAGATTAAAAAGAAAAAACTGGAAGCTGATTAAACAAAGATTATGTCAAGAGGTAGTAACAAAGGATCTTTTTGT
TGTAGATGGTTTTTATAATCATGATGAGAGATATACCATAGCGGTTAGACTAATAACTACCCAAGCTTCAGCTGCATATT
TTTTTAAGTTGATATCGATAACTCCGAGTGAAAAAGAATTACAGTCATTTGAGCCACAATGGGTAATAATGCATTCCCCT
CAAACACAGATAGAAGATTACCAAGACCTTGATTTAAATTCTTCAAAAGTAATAGCAACTAACCTTAAGCAGCGAGAAAG
TATATTGGTTGGGACTATGTATCTTGCTGAGATAAATAAGGTATTGTTGTCGATTATGAGCTATTATCTCTTGCTCAACG
ATATTGGCGTATTTTACTGTGCTGTCAGTGTTGATGCTGAAGCTAATAGTACAATATTTTTTGGTTTGTCAGGTAGCGGT
AAAACAACGTTAGCTTTAGACCAAAATAAGAGTCTTGTTGCAAATGAGGCTATTGCTTGGACAGAAATGCGTGGTGTGTA
TAGTCTAGAGTCAGGTTTGACAATTAAATCAGCAAGTTTTAAAAAAGACGATCCGCGTATCAAACAAGCATTAGCTGGGG
ACCTTTTGATAGAAAATCCTAACTTTGATGATAGTCACAATATTATATTTGGTGAGAAAAATAGTTCACAAAGTAATACT
TATGTAACTTTCCCACGTGAAAATTTTGTTAATGTTATAAACACTGATAACCCTAACACAATAATTTTTCTTGTCAAAGA
TGCAAAAGGTGTATTACCAAGAGTTGCAAAACTTAGTAAAGGTCAGGCAATTTACTATTTTTTATCTGGATATACTTCTA
CATCTATAGGAGTAGAGGCAGGTGTTACAGAGCCTAAACCAGAGTTTACAAGTTGCTATGCTCAGCCATTTCTATTACTT
AAGCCAACTAGATATGCAAGTATATTGCGTCAGCGTTTGAAGCATAGTAATGCTAAAATTTATATGATAAATGTTGGCTG
GATTGAGGGTGATTATAAGACAGGTAGAAGGGTTCCTGTTGAAGAGACAAAACTTATTGTTAACTACTTATTGACTAAAC
CAGAGAATGTTAGCTTTAAATTTACACGTCAAAAATATTTTAACTTTAAAGCTTTAACTTCAATAAATGATAATGGTCAA
GAGCTTCAACTTACCAATAATTGGAGTGATAGTGCTGAATATAAAAAAGAATATAAGTCTTTAGCAAGAGCTTTTATCAA
AAACTATGAGCAGTTTGAAAATGATGATTTTGCACTAAAATATAAAAAATTTGAACCAATTATTTAG

Upstream 100 bases:

>100_bases
AATAAAAAAACAATAACATATTTTTTTGAAAAAAAAGTATATACTAGCTATAATTTTTACTTAAAATCTAATTGCTCTAT
AAGCTAAGGGACTTTTGTTA

Downstream 100 bases:

>100_bases
ACGCTAATTTTGACTCTATTAAAAAAATAACATATCTATTATAATACTCCAAGGTCATTAAACATTTTAAATATATGCTG
ATTTATCTTTTTGAATGGTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVGSGEIKGRLPDDKYIVETKYAKKNIWWSENGSD
NKRLKRKNWKLIKQRLCQEVVTKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSP
QTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSGSG
KTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQSNT
YVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLL
KPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDNGQ
ELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII

Sequences:

>Translated_528_residues
MDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVGSGEIKGRLPDDKYIVETKYAKKNIWWSENGSD
NKRLKRKNWKLIKQRLCQEVVTKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSP
QTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSGSG
KTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQSNT
YVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLL
KPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDNGQ
ELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
>Mature_528_residues
MDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVGSGEIKGRLPDDKYIVETKYAKKNIWWSENGSD
NKRLKRKNWKLIKQRLCQEVVTKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSP
QTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSGSG
KTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQSNT
YVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLL
KPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDNGQ
ELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=479, Percent_Identity=32.5678496868476, Blast_Score=287, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6322950, Length=529, Percent_Identity=31.1909262759924, Blast_Score=264, Evalue=2e-71,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 60477; Mature: 60477

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVGSGEIKGRLPDDK
CCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCEECCCCCCC
YIVETKYAKKNIWWSENGSDNKRLKRKNWKLIKQRLCQEVVTKDLFVVDGFYNHDERYTI
EEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECEECCCCEEEE
AVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSPQTQIEDYQDLDLNSSKVIAT
EEEEEEECCCCEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEEEE
NLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSGSG
CCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCC
KTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENP
CEEEEECCCCCEECCCCEEEHHHCCEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEECC
NFDDSHNIIFGEKNSSQSNTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSK
CCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHCCC
GQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLLKPTRYASILRQRLKHSNAKI
CCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCEE
YMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDNGQ
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEECCCCCC
ELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVGSGEIKGRLPDDK
CCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCEECCCCCCC
YIVETKYAKKNIWWSENGSDNKRLKRKNWKLIKQRLCQEVVTKDLFVVDGFYNHDERYTI
EEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECEECCCCEEEE
AVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSPQTQIEDYQDLDLNSSKVIAT
EEEEEEECCCCEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEEEE
NLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSGSG
CCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCC
KTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENP
CEEEEECCCCCEECCCCEEEHHHCCEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEECC
NFDDSHNIIFGEKNSSQSNTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSK
CCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHCCC
GQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLLKPTRYASILRQRLKHSNAKI
CCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCEE
YMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDNGQ
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEECCCCCC
ELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA