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The map label for this gene is ligA [H]

Identifier: 57238680

GI number: 57238680

Start: 1732089

End: 1732937

Strand: Reverse

Name: ligA [H]

Synonym: CJE1841

Alternate gene names: 57238680

Gene position: 1732937-1732089 (Counterclockwise)

Preceding gene: 57238681

Following gene: 57238679

Centisome position: 97.47

GC content: 29.92

Gene sequence:

>849_bases
ATGAGGTTTGTTTTTTTAATATGTTGTGCTTGTTTAGTTTTTGCTAATGAAATTTTACTTCTTAGCAAATTCGATAAGCA
AGATTTTAACTCTAAAGATTTTAATGCTTATTTGATGAGCGAAAAACTTGATGGAGTGCGTGGAATTTGGGATGGGAAAT
ATCTAAAAACTCGCCAAAATTACAAAATAAAAACTCCTGACTTTTTTACAAAAAATTTCCCTCCTTTTGCTATTGATGGT
GAGCTTTGGATAGCAAGAAATAAATTTGATGAAATTTCAGCTTTAATTCGTAGTGGCGATAGTAATTTGACCTTATGGAA
GGAAGTAACTTATAATATTTTTGATGTGCCAAATGCTTGCGAAGAATTTCAAATTTCTACTTGTACTCTTAAAAATTGTT
TGGCTGTTTTAGAAGAGTATTTGCAAAAATATCCTAGTGCCTATATAAAAATTATTTCTCAAATTCCAGTTGAAAATCAA
AATAATTTAAATCAATTTTACGAAAGCATTATAAAAAATCAAGGTGAAGGTATTGTCATACGCAAAAATTTAAGTCCTTA
TGAAAAAGGCAGAAGTAAAAATGCTATGAAATTAAAACCCTATGATGATGCAGAGTGTGAGTTAGTAGGATTTCGCAAGG
GCAAGGGTAAATTTGAAAATCAAGTAGGTGCCTTACTTTGTAAAATGCCTAATGGACAAATCATTAAAATAGGCAGTGGT
TTAAAAGATGAAGATAGAAAAAATCCTCCAAAAATTGGTAGTATTGTGACTTATAAATTTAATGGATTGACCAAAAATTC
CTTACCTCGCTTTCCGGTTTTTTTAAGAATAAGAGATGAAAATCCTTGA

Upstream 100 bases:

>100_bases
GATCTCATAAAGGATATTATCGACTTGTAATATATCTTGATGGAACTTATAATTATAGTATTCAAAAAGATGCAACTGGA
TATATGATTAATCTGCTTTG

Downstream 100 bases:

>100_bases
CTTTAAAATTATTTTGTGATAAAATATTTCCTTTTATTTATTCGGGGTGTAGCGCAGTCTGGTTAGCGCACTTGGTTTGG
GACCAAGGGGCCGAAGGTTC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRFVFLICCACLVFANEILLLSKFDKQDFNSKDFNAYLMSEKLDGVRGIWDGKYLKTRQNYKIKTPDFFTKNFPPFAIDG
ELWIARNKFDEISALIRSGDSNLTLWKEVTYNIFDVPNACEEFQISTCTLKNCLAVLEEYLQKYPSAYIKIISQIPVENQ
NNLNQFYESIIKNQGEGIVIRKNLSPYEKGRSKNAMKLKPYDDAECELVGFRKGKGKFENQVGALLCKMPNGQIIKIGSG
LKDEDRKNPPKIGSIVTYKFNGLTKNSLPRFPVFLRIRDENP

Sequences:

>Translated_282_residues
MRFVFLICCACLVFANEILLLSKFDKQDFNSKDFNAYLMSEKLDGVRGIWDGKYLKTRQNYKIKTPDFFTKNFPPFAIDG
ELWIARNKFDEISALIRSGDSNLTLWKEVTYNIFDVPNACEEFQISTCTLKNCLAVLEEYLQKYPSAYIKIISQIPVENQ
NNLNQFYESIIKNQGEGIVIRKNLSPYEKGRSKNAMKLKPYDDAECELVGFRKGKGKFENQVGALLCKMPNGQIIKIGSG
LKDEDRKNPPKIGSIVTYKFNGLTKNSLPRFPVFLRIRDENP
>Mature_282_residues
MRFVFLICCACLVFANEILLLSKFDKQDFNSKDFNAYLMSEKLDGVRGIWDGKYLKTRQNYKIKTPDFFTKNFPPFAIDG
ELWIARNKFDEISALIRSGDSNLTLWKEVTYNIFDVPNACEEFQISTCTLKNCLAVLEEYLQKYPSAYIKIISQIPVENQ
NNLNQFYESIIKNQGEGIVIRKNLSPYEKGRSKNAMKLKPYDDAECELVGFRKGKGKFENQVGALLCKMPNGQIIKIGSG
LKDEDRKNPPKIGSIVTYKFNGLTKNSLPRFPVFLRIRDENP

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 32440; Mature: 32440

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00333 DNA_LIGASE_A2 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVFLICCACLVFANEILLLSKFDKQDFNSKDFNAYLMSEKLDGVRGIWDGKYLKTRQN
CHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCEEECCCC
YKIKTPDFFTKNFPPFAIDGELWIARNKFDEISALIRSGDSNLTLWKEVTYNIFDVPNAC
CEECCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHCCCCCEEEEEEECEEEECCCCCH
EEFQISTCTLKNCLAVLEEYLQKYPSAYIKIISQIPVENQNNLNQFYESIIKNQGEGIVI
HHHEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEE
RKNLSPYEKGRSKNAMKLKPYDDAECELVGFRKGKGKFENQVGALLCKMPNGQIIKIGSG
ECCCCHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHCCEEEEEECCCCCEEEECCC
LKDEDRKNPPKIGSIVTYKFNGLTKNSLPRFPVFLRIRDENP
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRFVFLICCACLVFANEILLLSKFDKQDFNSKDFNAYLMSEKLDGVRGIWDGKYLKTRQN
CHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCEEECCCC
YKIKTPDFFTKNFPPFAIDGELWIARNKFDEISALIRSGDSNLTLWKEVTYNIFDVPNAC
CEECCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHCCCCCEEEEEEECEEEECCCCCH
EEFQISTCTLKNCLAVLEEYLQKYPSAYIKIISQIPVENQNNLNQFYESIIKNQGEGIVI
HHHEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEE
RKNLSPYEKGRSKNAMKLKPYDDAECELVGFRKGKGKFENQVGALLCKMPNGQIIKIGSG
ECCCCHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHCCEEEEEECCCCCEEEECCC
LKDEDRKNPPKIGSIVTYKFNGLTKNSLPRFPVFLRIRDENP
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]