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The map label for this gene is dnaG [H]

Identifier: 57238649

GI number: 57238649

Start: 1704254

End: 1706071

Strand: Direct

Name: dnaG [H]

Synonym: CJE1810

Alternate gene names: 57238649

Gene position: 1704254-1706071 (Clockwise)

Preceding gene: 57238644

Following gene: 57238650

Centisome position: 95.86

GC content: 28.44

Gene sequence:

>1818_bases
ATGATAACTAAAGAAAGTATAGAAAATTTATCCCAAAGACTTAATATTGTTGATATTATAGAAAATTATATTGAAGTAAA
AAAACAAGGCTCTAGTTTTGTATGTATCTGTCCTTTTCATGCAGATAAAAACCCGTCTATGCATATCAATCCTACCAAAG
GTTTTTATCATTGTTTTGCTTGCAAAGCTGGTGGAGATGCTTTTAAATTTGTAATGGATTATGAAAAATTAAGTTTTGCT
GATGCAGTTGAAAAAGTTGCAAGCCTTAGTAATTTTACTTTAAGTTATACAAAAGAAAAACAAGAAAATAAAAAAGAGCT
AAAGTCTATACTACCAAGCTTAAATGCGTATTTTAAAGATAATCTCAAACACCACAAAGAAGTCTTAACCTATCTTTACC
AAAGAGCTTTAAATGACAAAGATATAGCCAAATTCGAATTAGGTTTTGCAGGAGCAAGTGAAGATAGTATAAGATTATTG
CGAAATCAAAAAATTCCTTTAGAAGATGCTATGAGTGTTGGAGCTTTAAAAAAAGATGAAAATAATGAATTTTACGCAAG
TTTTATATGGCGTATCACCTTTCCAATCTACGATCATAAAGACTTATTAGTAGGGTTTGGAGGGCGTACTTTAAATCCTA
ATGTACCGGCAAAGTATGTAAATTCACCCCAAAATATACTTTTTGATAAAAGTAGAATTTTTTATGCTTTTAACATCGCT
AAAGAAAATATAGCCAAAAAAAAAGAAATAATAGTATGTGAAGGCTACATGGATGCAATAGCTTTTCACAAAGCAGGGTT
TAACAATGCTGTAGCAGTTCTTGGAACAGCATTAACCGAACATCATCTACCCTTAATCCGCCGTTATGATGCAAAAGTGA
TTTTATGTTTTGACAATGATGAAGCAGGATTAAAAGCTGCGACACGATCTGCATTTTTATTGAGCACAAACAAAATAGAC
GGTAAAGTGGCAATTTTGCAAGGCGGAAAAGATCCTGCTGAGCTCGTAGCAAAAAATGAAAGCACAAAACTTCATGATAT
ACTCGATGAAGGAATAGAGCTTGGTGAATTTTATATCAGACGCTTAATATCCACTCATTCCATAATTTCTGCTCTTGATA
AACAAAAAGCTTTAGAAGCTATACAAAAATTCACCTTTAATCTTGAACCTTTAGTGGCAAATTCTTACACTAGTCTAGTT
TCAAATTTATTAAAAGTGGATGAAAAATTTATTGTCTTATCGCAAAATTCAAAAAAAACCATACATACCCCTTTAATTTC
TCAAAATAAATACAATTTTCCTAAAGAAAAAATTCACATTGCAGAATTAGAACTTATAGCTTTTTTAAAACAGCATCCAG
ATATATGTAACGATTTTAAGCAAATTAGTGATAGTGTTTGTTTTAAGCATAAGATTTTATTGGATAAAATTTTAGAAAAA
AAAGGTTATGAAGATAGTGATATTAGAGAATTTGAAAATAAAAATATTCGAAAAAATTTAAACTATAGCGAATTCTTGTT
AGGTATTTGCAAGGTTAATTTAGCTTTTTTAAACAATGTAAAAATTAAAAATTCTACACTCGCATTAAAAAAACAACTTT
TTACGCTTATTGATAAAAATTTAAATCTTCTAATTAAAAATTTAAATACCGAAGAATTAAATAATTTTTTAAAAGAATAT
TTAAGCTTCTTAAAATATGAAAAAAACGAAGAAATTCTGCAACAATCATTTAGAAACTTAAATGGAATTTTTAGTAACAA
AAACTTTACAGCTTATGATTTAGGATTTAGCACACAAGATAATGATGAACCTTTTTAA

Upstream 100 bases:

>100_bases
GGTCTCTGTATTCTACAAAGAAAAAATCCATAGTCTCGCTCTAAAAATTTTATGCCAAATTATAGCCAAATCTTTGTATA
ATTTTAACAAGGTTTGTAAA

Downstream 100 bases:

>100_bases
TTTATTTAAATTTCAAGCCTTAATTTACCTTTTTTTAAACACAATCACGCTAAAGTATATAAAAATTTTTAAGGATAAAT
TATGATGCCTTTTAGCGATG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVASLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
RNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSAFLLSTNKID
GKVAILQGGKDPAELVAKNESTKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALEAIQKFTFNLEPLVANSYTSLV
SNLLKVDEKFIVLSQNSKKTIHTPLISQNKYNFPKEKIHIAELELIAFLKQHPDICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFENKNIRKNLNYSEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLLIKNLNTEELNNFLKEY
LSFLKYEKNEEILQQSFRNLNGIFSNKNFTAYDLGFSTQDNDEPF

Sequences:

>Translated_605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVASLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
RNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSAFLLSTNKID
GKVAILQGGKDPAELVAKNESTKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALEAIQKFTFNLEPLVANSYTSLV
SNLLKVDEKFIVLSQNSKKTIHTPLISQNKYNFPKEKIHIAELELIAFLKQHPDICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFENKNIRKNLNYSEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLLIKNLNTEELNNFLKEY
LSFLKYEKNEEILQQSFRNLNGIFSNKNFTAYDLGFSTQDNDEPF
>Mature_605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVASLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
RNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSAFLLSTNKID
GKVAILQGGKDPAELVAKNESTKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALEAIQKFTFNLEPLVANSYTSLV
SNLLKVDEKFIVLSQNSKKTIHTPLISQNKYNFPKEKIHIAELELIAFLKQHPDICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFENKNIRKNLNYSEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLLIKNLNTEELNNFLKEY
LSFLKYEKNEEILQQSFRNLNGIFSNKNFTAYDLGFSTQDNDEPF

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=375, Percent_Identity=33.8666666666667, Blast_Score=225, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69156; Mature: 69156

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
CKAGGDAFKFVMDYEKLSFADAVEKVASLSNFTLSYTKEKQENKKELKSILPSLNAYFKD
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
NLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLLRNQKIPLEDAMSVGALKKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
NNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
CCCEEEEEEEEEEECEECCCCEEEECCCCCCCCCCCHHHCCCCHHHEEECCEEEEEEEEH
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDND
HHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCEEEEEECCC
EAGLKAATRSAFLLSTNKIDGKVAILQGGKDPAELVAKNESTKLHDILDEGIELGEFYIR
CCCHHHHHCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RLISTHSIISALDKQKALEAIQKFTFNLEPLVANSYTSLVSNLLKVDEKFIVLSQNSKKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCE
IHTPLISQNKYNFPKEKIHIAELELIAFLKQHPDICNDFKQISDSVCFKHKILLDKILEK
EECCCCCCCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYEDSDIREFENKNIRKNLNYSEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKN
CCCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCC
LNLLIKNLNTEELNNFLKEYLSFLKYEKNEEILQQSFRNLNGIFSNKNFTAYDLGFSTQD
CEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCEEEEECCCCCCC
NDEPF
CCCCC
>Mature Secondary Structure
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
CKAGGDAFKFVMDYEKLSFADAVEKVASLSNFTLSYTKEKQENKKELKSILPSLNAYFKD
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
NLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLLRNQKIPLEDAMSVGALKKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
NNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
CCCEEEEEEEEEEECEECCCCEEEECCCCCCCCCCCHHHCCCCHHHEEECCEEEEEEEEH
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDND
HHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCEEEEEECCC
EAGLKAATRSAFLLSTNKIDGKVAILQGGKDPAELVAKNESTKLHDILDEGIELGEFYIR
CCCHHHHHCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RLISTHSIISALDKQKALEAIQKFTFNLEPLVANSYTSLVSNLLKVDEKFIVLSQNSKKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCE
IHTPLISQNKYNFPKEKIHIAELELIAFLKQHPDICNDFKQISDSVCFKHKILLDKILEK
EECCCCCCCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYEDSDIREFENKNIRKNLNYSEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKN
CCCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCC
LNLLIKNLNTEELNNFLKEYLSFLKYEKNEEILQQSFRNLNGIFSNKNFTAYDLGFSTQD
CEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCEEEEECCCCCCC
NDEPF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]