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The map label for this gene is rnhA

Identifier: 57238647

GI number: 57238647

Start: 1702771

End: 1703211

Strand: Reverse

Name: rnhA

Synonym: CJE1808

Alternate gene names: 57238647

Gene position: 1703211-1702771 (Counterclockwise)

Preceding gene: 57238648

Following gene: 57238646

Centisome position: 95.8

GC content: 32.88

Gene sequence:

>441_bases
ATGAAACACATTGAAATTTATACAGATGGTTCATGTCTTAATAATCCAGGTTTTGGCGGATGGGCTTATATTTTGCGTTA
TAAGGAGTATCAAAAGGAAGGTTTTGGAGCTGAGGCTAATACCACTAACAATAGAATGGAGCTAATGGCAATTATTGAAT
CTTTGAAGGCTTTAAAAGAGCCTTGTGAAATTTCGCTTTTTACAGATTCTAACCTAATGGTTCAAAGTATTAATGAATGG
CTAGAAGGATGGATAAAAAAGGATTTTAAAGGTAAAAAAAATATAGATTTATGGAAGGAATATATTAAAGTGGCAAAATC
TCATAAGATTAAGGCTTTTTGGGTAAAAGCACATAATGGACATTTGGAAAATGAAAGGTGTGATACATTGGCGCGTGAAG
CTGCTTTGAAAATTGCAAGGGAAAATGATGAAAAACATTGA

Upstream 100 bases:

>100_bases
CGTTGTTCTGAGTGTAAAAATGTTATGCCTTTGTTTTTTTATCACTGTCCTGTTTGTTATGAGTTTAATACTTGTAAAAT
TATTTATGAAGTGAAAAACA

Downstream 100 bases:

>100_bases
AAAACTAGAGCGGAGTTTAACTTATGAATTTAAAGATAAAAATCTTCTCATACATGCTTTAACCCATAAAAGTTTTAAAA
AATCTTACAACAACGAACGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKHIEIYTDGSCLNNPGFGGWAYILRYKEYQKEGFGAEANTTNNRMELMAIIESLKALKEPCEISLFTDSNLMVQSINEW
LEGWIKKDFKGKKNIDLWKEYIKVAKSHKIKAFWVKAHNGHLENERCDTLAREAALKIARENDEKH

Sequences:

>Translated_146_residues
MKHIEIYTDGSCLNNPGFGGWAYILRYKEYQKEGFGAEANTTNNRMELMAIIESLKALKEPCEISLFTDSNLMVQSINEW
LEGWIKKDFKGKKNIDLWKEYIKVAKSHKIKAFWVKAHNGHLENERCDTLAREAALKIARENDEKH
>Mature_146_residues
MKHIEIYTDGSCLNNPGFGGWAYILRYKEYQKEGFGAEANTTNNRMELMAIIESLKALKEPCEISLFTDSNLMVQSINEW
LEGWIKKDFKGKKNIDLWKEYIKVAKSHKIKAFWVKAHNGHLENERCDTLAREAALKIARENDEKH

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Escherichia coli, GI1786408, Length=147, Percent_Identity=50.3401360544218, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=33.5714285714286, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552509, Length=152, Percent_Identity=32.8947368421053, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137476, Length=152, Percent_Identity=32.8947368421053, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_CAMJ8 (A8FNU8)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001483112.1
- ProteinModelPortal:   A8FNU8
- SMR:   A8FNU8
- STRING:   A8FNU8
- GeneID:   5617873
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_1538
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   CJEJ407148:C8J_1538-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16979; Mature: 16979

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIEIYTDGSCLNNPGFGGWAYILRYKEYQKEGFGAEANTTNNRMELMAIIESLKALKE
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEISLFTDSNLMVQSINEWLEGWIKKDFKGKKNIDLWKEYIKVAKSHKIKAFWVKAHNG
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCC
HLENERCDTLAREAALKIARENDEKH
CCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKHIEIYTDGSCLNNPGFGGWAYILRYKEYQKEGFGAEANTTNNRMELMAIIESLKALKE
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEISLFTDSNLMVQSINEWLEGWIKKDFKGKKNIDLWKEYIKVAKSHKIKAFWVKAHNG
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCC
HLENERCDTLAREAALKIARENDEKH
CCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA