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The map label for this gene is dapD [C]

Identifier: 57238618

GI number: 57238618

Start: 1673507

End: 1674667

Strand: Reverse

Name: dapD [C]

Synonym: CJE1777

Alternate gene names: 57238618

Gene position: 1674667-1673507 (Counterclockwise)

Preceding gene: 57238619

Following gene: 57238600

Centisome position: 94.2

GC content: 32.99

Gene sequence:

>1161_bases
ATGATTAATACTAAAGAAGATTTTTTACTTTTAATCAAACAAATCGAGCAAAAAAGTGGCTATAAAAAACCTAAAGCTTT
TGGTATAGCAAGGCTTGATAGAGGACAGTTAAATAAAAATAAAATCTTACAAGCAAGTTTTGCTTTGATTAACTATGAGC
AAAATTTTGGCTCAGCGGCAATCATGCTTGAAGCTTTTATGCAAAGGGGCGTGGAGATTGATTTTAATGCGAGTGAGTTT
GTGCAAACTTTGAAATTAGAAGATATTGATTTTGCACTTTCTTGTTTTAAACCTTTTTTAGAAGAAGATGGGCATCAAAA
TATTGATTTATTAAAGATTATCAAAGATAAATTTAAAGACGATGAATTTTCTTTTGTTTGTCTTTTTGAAGATAAAGAAC
CTTTAAGTGTAGAAAGTATTTATCTAAAACTTTATTTGCTTTCTACTAAAAAAGTACCTTTAAGAAGTATCAATCTAAAT
GGATCTTTTGGACTTTTAAGCAATGTAGCTTGGAGTGATGATAAGCCTATCGAGCTTGAGTATTTAAGAGCAAATGAAAT
GCGTTTAAAAATGAGCAATCAATATCCAAAAATCGATTTTGTTGATAAATTCCCAAGATTTTTAGCACATATTATCCCTG
AGGATAATACAAGAATTTTAGAAAGTTCTAAAGTAAGAATGGGTGCGTCTTTAGCAGCAGGAACAACCATCATGCCAGGG
GCAAGTTATGTGAATTTTAATGCAGGTACAACAGGAGCTTGTATGGTTGAAGGGCGTATTAGTTCAAGTGCTATAGTAGG
AGAAGGTTCTGATGTAGGTGGCGGAGCTTCGATTTTAGGTGTTTTAAGTGGAACAAGTGGCAATGCTATAAGCGTAGGCA
AGGCTTGTCTTTTAGGGGCTAATTCAGTGACAGGAATTCCTTTAGGGGATAATTGTATAGTTGATGCAGGTATTGCAGTG
CTAGAAGGAACGAAATTTTTACTCAAAGATGCCGAAGAACTTGCTAAACTTAATCCTTATTTTAATTTTGATAAAGAAAT
TTACAAAGGCTTAGAGCTTAAAGGTTTAAATGGGCTACATTTCCGTCAAGATTCTATAAGTGGTGCTATGATAGTAGCTT
TAAATAAAAAAGCAGTCAAACTTAATGAAGCCTTGCATTGA

Upstream 100 bases:

>100_bases
ATTAACAATGAAGGTGGTGCGGATAATTCTGCTATACAACCTATGATGAATGGAAAAAGTGCGTGTTCGCATTAAAAATT
AAGTGGATAAGGAAAAGAAA

Downstream 100 bases:

>100_bases
TATAAATTTATTTTACATAAAGCTTTAAGCTTTATGTCTTTCTTTTAATTTTGAAATCACATCTGAGAAACTAAGATTTG
CATCTGCAAGTAAAACACTT

Product: 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase

Products: Coenzyme A; N-Succinyl-2-L-amino-6-oxoheptanedioate [C]

Alternate protein names: Transferase; Tetrahydrodipicolinate Succinylase; Tetrahydropicolinate Succinylase; Tetrahydrodipicolinate N- Succinyltransferase; Transferase Hexapeptide Repeat Containing Protein; Succinyltransferase; Succinyl-Transferase; Tranferase; Tetrahydrodipicolinate N-Succinyltransferase-Like Protein; Lysine Biosynthesis-Like Protein; Transferase Tetrahydrodipicolinate N-Succinyltransferase

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MINTKEDFLLLIKQIEQKSGYKKPKAFGIARLDRGQLNKNKILQASFALINYEQNFGSAAIMLEAFMQRGVEIDFNASEF
VQTLKLEDIDFALSCFKPFLEEDGHQNIDLLKIIKDKFKDDEFSFVCLFEDKEPLSVESIYLKLYLLSTKKVPLRSINLN
GSFGLLSNVAWSDDKPIELEYLRANEMRLKMSNQYPKIDFVDKFPRFLAHIIPEDNTRILESSKVRMGASLAAGTTIMPG
ASYVNFNAGTTGACMVEGRISSSAIVGEGSDVGGGASILGVLSGTSGNAISVGKACLLGANSVTGIPLGDNCIVDAGIAV
LEGTKFLLKDAEELAKLNPYFNFDKEIYKGLELKGLNGLHFRQDSISGAMIVALNKKAVKLNEALH

Sequences:

>Translated_386_residues
MINTKEDFLLLIKQIEQKSGYKKPKAFGIARLDRGQLNKNKILQASFALINYEQNFGSAAIMLEAFMQRGVEIDFNASEF
VQTLKLEDIDFALSCFKPFLEEDGHQNIDLLKIIKDKFKDDEFSFVCLFEDKEPLSVESIYLKLYLLSTKKVPLRSINLN
GSFGLLSNVAWSDDKPIELEYLRANEMRLKMSNQYPKIDFVDKFPRFLAHIIPEDNTRILESSKVRMGASLAAGTTIMPG
ASYVNFNAGTTGACMVEGRISSSAIVGEGSDVGGGASILGVLSGTSGNAISVGKACLLGANSVTGIPLGDNCIVDAGIAV
LEGTKFLLKDAEELAKLNPYFNFDKEIYKGLELKGLNGLHFRQDSISGAMIVALNKKAVKLNEALH
>Mature_386_residues
MINTKEDFLLLIKQIEQKSGYKKPKAFGIARLDRGQLNKNKILQASFALINYEQNFGSAAIMLEAFMQRGVEIDFNASEF
VQTLKLEDIDFALSCFKPFLEEDGHQNIDLLKIIKDKFKDDEFSFVCLFEDKEPLSVESIYLKLYLLSTKKVPLRSINLN
GSFGLLSNVAWSDDKPIELEYLRANEMRLKMSNQYPKIDFVDKFPRFLAHIIPEDNTRILESSKVRMGASLAAGTTIMPG
ASYVNFNAGTTGACMVEGRISSSAIVGEGSDVGGGASILGVLSGTSGNAISVGKACLLGANSVTGIPLGDNCIVDAGIAV
LEGTKFLLKDAEELAKLNPYFNFDKEIYKGLELKGLNGLHFRQDSISGAMIVALNKKAVKLNEALH

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fourth step. [C]

COG id: COG2171

COG function: function code E; Tetrahydrodipicolinate N-succinyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.117 [C]

Molecular weight: Translated: 42377; Mature: 42377

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTKEDFLLLIKQIEQKSGYKKPKAFGIARLDRGQLNKNKILQASFALINYEQNFGSAA
CCCCHHHHHHHHHHHHHHCCCCCCHHHCEEECCCCCCCCCCEEEEEEEEEEECCCCCHHH
IMLEAFMQRGVEIDFNASEFVQTLKLEDIDFALSCFKPFLEEDGHQNIDLLKIIKDKFKD
HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
DEFSFVCLFEDKEPLSVESIYLKLYLLSTKKVPLRSINLNGSFGLLSNVAWSDDKPIELE
CCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCHHHCCCCCCCCCCEEEE
YLRANEMRLKMSNQYPKIDFVDKFPRFLAHIIPEDNTRILESSKVRMGASLAAGTTIMPG
EEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEECCCHHCCCCEECCC
ASYVNFNAGTTGACMVEGRISSSAIVGEGSDVGGGASILGVLSGTSGNAISVGKACLLGA
CCEEEECCCCCCEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEECCCEEEECC
NSVTGIPLGDNCIVDAGIAVLEGTKFLLKDAEELAKLNPYFNFDKEIYKGLELKGLNGLH
CCCCCCCCCCCCEEECCEEECCCCHHHHHCHHHHHHCCCCCCCCHHHHCCCEECCCCCCE
FRQDSISGAMIVALNKKAVKLNEALH
EECCCCCCEEEEEECCCEEEHHHCCC
>Mature Secondary Structure
MINTKEDFLLLIKQIEQKSGYKKPKAFGIARLDRGQLNKNKILQASFALINYEQNFGSAA
CCCCHHHHHHHHHHHHHHCCCCCCHHHCEEECCCCCCCCCCEEEEEEEEEEECCCCCHHH
IMLEAFMQRGVEIDFNASEFVQTLKLEDIDFALSCFKPFLEEDGHQNIDLLKIIKDKFKD
HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
DEFSFVCLFEDKEPLSVESIYLKLYLLSTKKVPLRSINLNGSFGLLSNVAWSDDKPIELE
CCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCHHHCCCCCCCCCCEEEE
YLRANEMRLKMSNQYPKIDFVDKFPRFLAHIIPEDNTRILESSKVRMGASLAAGTTIMPG
EEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEECCCHHCCCCEECCC
ASYVNFNAGTTGACMVEGRISSSAIVGEGSDVGGGASILGVLSGTSGNAISVGKACLLGA
CCEEEECCCCCCEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCEEECCCEEEECC
NSVTGIPLGDNCIVDAGIAVLEGTKFLLKDAEELAKLNPYFNFDKEIYKGLELKGLNGLH
CCCCCCCCCCCCEEECCEEECCCCHHHHHCHHHHHHCCCCCCCCHHHHCCCEECCCCCCE
FRQDSISGAMIVALNKKAVKLNEALH
EECCCCCCEEEEEECCCEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.022 {tetrahydrodipicolinate}} 0.015 {succinyl-CoA}} [C]

Substrates: H2O; Succinyl-CoA; 2,3,4,5-Tetrahydrodipicolinate [C]

Specific reaction: H2O + Succinyl-CoA + 2,3,4,5-Tetrahydrodipicolinate --> Coenzyme A + N-Succinyl-2-L-amino-6-oxoheptanedioate [C]

General reaction: Acyl group transfer [C]

Inhibitor: 2-Hydroxy tetrahydropyran-2, 6-dicarboxylic acid; CaCl2; CoCl2; CuSO4; MgCl2; MnCl2; N-Ethyl maleimide; p-Chloromercuriphenyl sulfonate; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA