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The map label for this gene is appB [H]

Identifier: 57238596

GI number: 57238596

Start: 1653221

End: 1654165

Strand: Reverse

Name: appB [H]

Synonym: CJE1754

Alternate gene names: 57238596

Gene position: 1654165-1653221 (Counterclockwise)

Preceding gene: 57238597

Following gene: 57238595

Centisome position: 93.04

GC content: 28.04

Gene sequence:

>945_bases
GTGCTAAAACGCTTAGTTTTTAGTATTTTCATAGCATTTTTTAGCACTTTTTTATGTTTTGTTTTACTTTATTTTAGTAA
AGGTAGTATAGCTTATGCTAATGGAGTTAATTCACAAAGTAAAGAATTTGTTCAAAGAATAGAACAAAATTTAGGGCTTG
ATAAGCCTTTGCTAGAGCAGTATAAAATTTGGCTTTTTAAGGCTTTAAAAGGAGATTTGGGAGTTTCTTTTTTAAGCGGA
GAAAGTGTTTTAAAGCTTATAAAAGAAAGAATTTTTAATACTTTTATTTTGGGTTTTAGCGCCTTAATGCTTTTATTTTT
ACTCAGTGTTTTTTTGGCACTTTTGGGATATTCTTATAAAGAAAGTTTTATAGATAAAATCATCACTTTTTTAGCTTTTA
ATTTTTTTACTTTACCTCCTTTTGTTTTGGCTTTATTGTTTGTTTTGGTTTTTGGAATTTTTTGGAAAATTTTGCCTGTT
ATGGGAAGTAGTGATATAGGCTTTGAAGATGATTTTTTAAATCGTTTAGAGCATTTGATTTTGCCTGTTTTGGTGCTCGT
TTTATCACATTTGGCGTTATTTTTAAGGATTGCTAGAAATTTTATCAATGAAAGTTTTTCTCAAATTTTTATTCAAAATT
TATACGCTAGAGCCTTAAGGGAAAAAGATATTTATTTTTTGGTTTTAAGATATTCTTTAAGCCCTATTATAGCTTATTTT
GGAGGCAGTGCTTTAAGTTTTATGATGGGAACTTATGTGGTTGAGAGTGTTTTTGCTTATGAGGGGCTTGGGTCTTTGCT
TTTTAAAAGTATTATTTTTAAAGATTATCCTGTTGTACTTGCACTTATATTTTTTAGCGTTTTGCTTGCAGCATTTTTTA
CATTTTTAAGTGATATTGTAGCTAGGATTTTAAATCCTAGATTAAGAAGGCTTGATTTTGTTTAA

Upstream 100 bases:

>100_bases
TTGCTTTAGTTTATAATAAAGATTTAAAAGGTATCAAAGCAAGGACTTTAGGTCATCACGGAGTGGGTTTTACTTGGAAT
GTTTATGAGTGGAGTAAATA

Downstream 100 bases:

>100_bases
ATTTCTTATCATTCTTGCGCCTTTAATTTTTTTGTTTTTATGCGCCTTGTTTGCACCTTTTTTGGCTCCTTTTGATATTT
TAAGCACTCATTTAGAAAAT

Product: peptide ABC transporter permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLKRLVFSIFIAFFSTFLCFVLLYFSKGSIAYANGVNSQSKEFVQRIEQNLGLDKPLLEQYKIWLFKALKGDLGVSFLSG
ESVLKLIKERIFNTFILGFSALMLLFLLSVFLALLGYSYKESFIDKIITFLAFNFFTLPPFVLALLFVLVFGIFWKILPV
MGSSDIGFEDDFLNRLEHLILPVLVLVLSHLALFLRIARNFINESFSQIFIQNLYARALREKDIYFLVLRYSLSPIIAYF
GGSALSFMMGTYVVESVFAYEGLGSLLFKSIIFKDYPVVLALIFFSVLLAAFFTFLSDIVARILNPRLRRLDFV

Sequences:

>Translated_314_residues
MLKRLVFSIFIAFFSTFLCFVLLYFSKGSIAYANGVNSQSKEFVQRIEQNLGLDKPLLEQYKIWLFKALKGDLGVSFLSG
ESVLKLIKERIFNTFILGFSALMLLFLLSVFLALLGYSYKESFIDKIITFLAFNFFTLPPFVLALLFVLVFGIFWKILPV
MGSSDIGFEDDFLNRLEHLILPVLVLVLSHLALFLRIARNFINESFSQIFIQNLYARALREKDIYFLVLRYSLSPIIAYF
GGSALSFMMGTYVVESVFAYEGLGSLLFKSIIFKDYPVVLALIFFSVLLAAFFTFLSDIVARILNPRLRRLDFV
>Mature_314_residues
MLKRLVFSIFIAFFSTFLCFVLLYFSKGSIAYANGVNSQSKEFVQRIEQNLGLDKPLLEQYKIWLFKALKGDLGVSFLSG
ESVLKLIKERIFNTFILGFSALMLLFLLSVFLALLGYSYKESFIDKIITFLAFNFFTLPPFVLALLFVLVFGIFWKILPV
MGSSDIGFEDDFLNRLEHLILPVLVLVLSHLALFLRIARNFINESFSQIFIQNLYARALREKDIYFLVLRYSLSPIIAYF
GGSALSFMMGTYVVESVFAYEGLGSLLFKSIIFKDYPVVLALIFFSVLLAAFFTFLSDIVARILNPRLRRLDFV

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=335, Percent_Identity=26.2686567164179, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=28.6624203821656, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787053, Length=313, Percent_Identity=25.2396166134185, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787497, Length=314, Percent_Identity=26.1146496815287, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788504, Length=293, Percent_Identity=26.962457337884, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787761, Length=289, Percent_Identity=29.4117647058824, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=24.2647058823529, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36024; Mature: 36024

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLVFSIFIAFFSTFLCFVLLYFSKGSIAYANGVNSQSKEFVQRIEQNLGLDKPLLEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHH
YKIWLFKALKGDLGVSFLSGESVLKLIKERIFNTFILGFSALMLLFLLSVFLALLGYSYK
HHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ESFIDKIITFLAFNFFTLPPFVLALLFVLVFGIFWKILPVMGSSDIGFEDDFLNRLEHLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LPVLVLVLSHLALFLRIARNFINESFSQIFIQNLYARALREKDIYFLVLRYSLSPIIAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GGSALSFMMGTYVVESVFAYEGLGSLLFKSIIFKDYPVVLALIFFSVLLAAFFTFLSDIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARILNPRLRRLDFV
HHHHCHHHHHHCCC
>Mature Secondary Structure
MLKRLVFSIFIAFFSTFLCFVLLYFSKGSIAYANGVNSQSKEFVQRIEQNLGLDKPLLEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHH
YKIWLFKALKGDLGVSFLSGESVLKLIKERIFNTFILGFSALMLLFLLSVFLALLGYSYK
HHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ESFIDKIITFLAFNFFTLPPFVLALLFVLVFGIFWKILPVMGSSDIGFEDDFLNRLEHLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LPVLVLVLSHLALFLRIARNFINESFSQIFIQNLYARALREKDIYFLVLRYSLSPIIAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GGSALSFMMGTYVVESVFAYEGLGSLLFKSIIFKDYPVVLALIFFSVLLAAFFTFLSDIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARILNPRLRRLDFV
HHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]