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The map label for this gene is 57237775

Identifier: 57237775

GI number: 57237775

Start: 965713

End: 966585

Strand: Reverse

Name: 57237775

Synonym: CJE1025

Alternate gene names: NA

Gene position: 966585-965713 (Counterclockwise)

Preceding gene: 57237776

Following gene: 57237773

Centisome position: 54.37

GC content: 33.1

Gene sequence:

>873_bases
ATGAAAATCGCTTTAATACAACAAAAATTTCATTTAAATAAAGAACAAACTATAAAAAAAACTTGCGAATTTATAGAAGA
AGCCTCTAAACAAGGAGCTGAGCTTGTTTGCTTAGGAGAACTTCATCAAAGTGAGTATTTTTGTCAAAGTGAAAATGTGG
ATTTTTTTGACTATGCTAATGATTATGAAAAAGATGTGAAATTTTGGGCAAATATAGCTAGAAAAAATCAAATAGTGCTT
ATTGCTTCATTGTTTGAAAAACGAAGTGCCGGACTTTATCATAATACTGCAGTGGTTTTTGAAAAAGATGGTTCTATAGC
AGGCAAATATCGCAAAATGCACATACCCGATGATCCTTGTTTTTATGAAAAATTTTATTTTACTCCTGGAGATTTGGGTT
TTGAACCTATAAATACAAGTTTAGGTAAGCTTGGAGTGCTTATTTGTTGGGATCAGTGGTATCCTGAAGCAGCAAGAATT
ATGGCTTTAAAAGGAGCTGAGATTCTTATTTATCCCACGGCAATTGGTTGGTTTGATAAGGATAAAGATGAAGAAAAACA
AAGACAGCTCAATGCTTGGCTTGGCGTACAAAAAGGACATGCTATAGCTAATGGTTTATATGTAGTAGCTATCAATAGGG
TAGGTTTTGAAAAAGATGTAAGCGGGGTAGAAGAGGGCATTAGATTTTGGGGAAATTCTTTTGTTTTTGGCCCTCAAGGT
GAAGAACTTTGTCTTTTAGATTCTCAAAACGAATGTGTAAAAATCATAGAAATAGATAAAAAAAGAAGTGAAAATGTGCG
TCGTTGGTGGCCTTTTTTGCGAGATAGACGCATAGAATATTTTGCAGATTTAACTAAAAGATTTATTGATTAA

Upstream 100 bases:

>100_bases
AAATAGAAGAAGGTGTGCGTAAGATGTTTGAAAATGAAAAATGGGATATACAAATTCATTTAGATCCTTATGATGATGCA
GAACAAGAAAGGCAAAGACA

Downstream 100 bases:

>100_bases
ATCAATAAATCTTATTTGTTTTGTATATTTTCAAGATAGCTTTTTATTTGCTCTTTTTCTTGTTCGTTTAAATTTATAAA
ACTTATTTCTTTAAGTCTTG

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKIALIQQKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYANDYEKDVKFWANIARKNQIVL
IASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPDDPCFYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARI
MALKGAEILIYPTAIGWFDKDKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFGPQG
EELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYFADLTKRFID

Sequences:

>Translated_290_residues
MKIALIQQKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYANDYEKDVKFWANIARKNQIVL
IASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPDDPCFYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARI
MALKGAEILIYPTAIGWFDKDKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFGPQG
EELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYFADLTKRFID
>Mature_290_residues
MKIALIQQKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYANDYEKDVKFWANIARKNQIVL
IASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPDDPCFYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARI
MALKGAEILIYPTAIGWFDKDKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFGPQG
EELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYFADLTKRFID

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=287, Percent_Identity=29.2682926829268, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI7706509, Length=305, Percent_Identity=25.2459016393443, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI297632348, Length=296, Percent_Identity=26.0135135135135, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI5031947, Length=296, Percent_Identity=26.0135135135135, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI297632350, Length=296, Percent_Identity=26.0135135135135, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI297632346, Length=185, Percent_Identity=27.5675675675676, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=305, Percent_Identity=27.2131147540984, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=25.6944444444444, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=297, Percent_Identity=26.9360269360269, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322335, Length=316, Percent_Identity=23.7341772151899, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358471, Length=289, Percent_Identity=28.719723183391, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355835, Length=295, Percent_Identity=25.7627118644068, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17933642, Length=286, Percent_Identity=22.027972027972, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33700; Mature: 33700

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALIQQKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYAN
CEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHCCCCEECCCCCCHHHCCC
DYEKDVKFWANIARKNQIVLIASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPDDPC
CHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCEECEEEEEECCCCCCCCEEEEECCCCCC
FYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARIMALKGAEILIYPTAIGWFDK
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHEEEECCCEEEEEEEEECCCCC
DKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFGPQG
CCCHHHHHHHHHHHCCCCCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
EELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYFADLTKRFID
CEEEEECCCCCEEEEEEECCHHCCCHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIALIQQKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYAN
CEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHCCCCEECCCCCCHHHCCC
DYEKDVKFWANIARKNQIVLIASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPDDPC
CHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCEECEEEEEECCCCCCCCEEEEECCCCCC
FYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARIMALKGAEILIYPTAIGWFDK
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHEEEECCCEEEEEEEEECCCCC
DKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFGPQG
CCCHHHHHHHHHHHCCCCCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
EELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYFADLTKRFID
CEEEEECCCCCEEEEEEECCHHCCCHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]