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The map label for this gene is pycB [H]

Identifier: 57237761

GI number: 57237761

Start: 945951

End: 947750

Strand: Reverse

Name: pycB [H]

Synonym: CJE1011

Alternate gene names: 57237761

Gene position: 947750-945951 (Counterclockwise)

Preceding gene: 57237762

Following gene: 57237760

Centisome position: 53.31

GC content: 32.67

Gene sequence:

>1800_bases
ATGGCTAAAAAATTCATCGATGTAATGGATACAAGCTTTAGGGACGGTTTTCAGTCTGTTTATGGAGCTAGGGTTTTAAT
GGATGATTTTTTTCCTGCAGTAGAAGCAGCAAAAGAAGCGGGTATTACTCATTTTGAATTTGGTGGTGGTGCTAGATTTC
AAAGCTTGTATTTTTATCTTAATGAAGATGCTTTTACCATGATGGATAGATTTAGGGCTATTGTAGGAAAAGATGCTAAT
CTTCAAACTTTAGCAAGAGGAGTCAATACCGTTACTTTAGATACTGGAAATAGTGAACTTATTGATTTGCATGCAAAACT
TTTTGCAAAACACGGTACTACAACGATAAGAAATTTTGATGCCCTTAATGATGTAAATAATTTAAAATTTAGTGGTGAAT
GTATAGTAAAACATGGGTTAAAGCATGAAATTACTATTACTTTAATGGATTTACCGTCAAATTGCAAAGGTGCTCATGAT
GTGCCTTTTTATGAAAAAATTTTAAAAGAAATTTTAACAGCAGAAATTCCTTTTCATAGTATTTGTTTCAAAGATGCAAG
TGGAACTTCTAATCCAAATAAAATTTATAAAACCATTAAAATGGCTAGAAAAATTTTACCTCAAGATACACATATTAGAC
TTCATACGCATGAAACTGCAGGGGTGAGTATAGCTTGCTATCTTGCAGCGCTTGAAGCAGGGGTTGATGGTATTGATTTG
GCTGCAGCTCCTGTAAGTGGTGGAACTTCTCAGCCTGATATTTTAACCATGATGCATGCTTTAAAAGGCAAAGATTATGA
TTTAGGTGGACTTGAAGAAGAAAAAATTTTAAGATACGAAGAAGTTTTAAAAGATTGTTTAAAAGAGTATTTTTTACCAC
CTGAAGCTACTATGGTAAATCCACTTATTCCTTTTTCTCCTATGCCAGGAGGAGCTTTAACAGCAAATACACAAATGATG
AGGGATAATAATATTTTAGATAAATTTCCACAGGTCATTCATGCTATGAGAGAAGTTGTGGAAAAAGGTGGATTTGGTAC
TTCGGTTACTCCAGTTTCTCAATTTTATTTTCAACAAGCATTTAATAATGTTATGTTTGGGCCTTGGAAAAAAATCGCAG
AAGGTTATGGAAAAATGGTGCTTGGTTATTTTGGAAAAACTCCAGTTACACCCGATGCAAACATTGTTGAACTTGCTTCA
AAACAACTTAATTTAGAACCCACTACAGAGCTTGCTATTAATATAGCCGATAAAGATGAGAGTAAAAGCATAGCTTATAC
TAAAACCTTGCTTGAAAAAGAGGGTATAGAAACGAGTGAAGAAAATATATTTATTGCAGCAGCTTGTAAAGAAAAAGGTA
TAGCATTTTTAAAAGGAGAGGCAAAGGTAAATATACGCAAATTAGCTAGCATGCCAAAGCCTATGAGTGTAGATGAAAAC
AAATTTACTGTAGCTGTAAATGGTAATAAATATCATGTGGAAGTGAGTTATGGTTTTGATAAAGATGTTAATGTTAAGAG
TGTAAAAAAAGTAGAAGAAAATAAAAATATTATTTCTTCAAATTCAACAAGTTCTGTCGATGCTGAAAATGAAATTTTAG
CAGGTATTTCAGGTAATGTCTTTAAAATTTATGTTAACGAAGGCGAAGAAGTAAAATCAGGTCAAGCTATCATGGTTTTA
GAAGCAATGAAGATGGAGATTGAGGTTAATGCCCCAAAAGATGGAATTATTTCAGAGCTTTGTATTAAAATAGGTGATAC
TGTTAATGAAGGCGAAGTATTAGCTATTTACAAGAATTAA

Upstream 100 bases:

>100_bases
TTGTTTTTTATTCGTTTTATATCGCCTATAGCAATAATTTTGGTAATGTGTTATCAAATTTTTGTATAATAATGAAAAAA
TATTTTTTTAAGGAAAAATA

Downstream 100 bases:

>100_bases
AGTGAGGGAAAAATGAAAAAATTTGATAAGTTAGGTTTGGATAATATAAAGGAAATTTTTCATAATTTAAGTTATGATGA
ATTAAATGCTCATGAAAAAG

Product: oxaloacetate decarboxylase, alpha subunit

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDAN
LQTLARGVNTVTLDTGNSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKHGLKHEITITLMDLPSNCKGAHD
VPFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYKTIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVDGIDL
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMM
RDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVTPDANIVELAS
KQLNLEPTTELAINIADKDESKSIAYTKTLLEKEGIETSEENIFIAAACKEKGIAFLKGEAKVNIRKLASMPKPMSVDEN
KFTVAVNGNKYHVEVSYGFDKDVNVKSVKKVEENKNIISSNSTSSVDAENEILAGISGNVFKIYVNEGEEVKSGQAIMVL
EAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN

Sequences:

>Translated_599_residues
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDAN
LQTLARGVNTVTLDTGNSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKHGLKHEITITLMDLPSNCKGAHD
VPFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYKTIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVDGIDL
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMM
RDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVTPDANIVELAS
KQLNLEPTTELAINIADKDESKSIAYTKTLLEKEGIETSEENIFIAAACKEKGIAFLKGEAKVNIRKLASMPKPMSVDEN
KFTVAVNGNKYHVEVSYGFDKDVNVKSVKKVEENKNIISSNSTSSVDAENEILAGISGNVFKIYVNEGEEVKSGQAIMVL
EAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
>Mature_598_residues
AKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDANL
QTLARGVNTVTLDTGNSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKHGLKHEITITLMDLPSNCKGAHDV
PFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYKTIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVDGIDLA
AAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMMR
DNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVTPDANIVELASK
QLNLEPTTELAINIADKDESKSIAYTKTLLEKEGIETSEENIFIAAACKEKGIAFLKGEAKVNIRKLASMPKPMSVDENK
FTVAVNGNKYHVEVSYGFDKDVNVKSVKKVEENKNIISSNSTSSVDAENEILAGISGNVFKIYVNEGEEVKSGQAIMVLE
AMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=628, Percent_Identity=25, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI106049295, Length=628, Percent_Identity=25, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI106049292, Length=628, Percent_Identity=25, Blast_Score=116, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17562816, Length=287, Percent_Identity=28.9198606271777, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319695, Length=639, Percent_Identity=25.508607198748, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321376, Length=641, Percent_Identity=24.0249609984399, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI281363050, Length=642, Percent_Identity=26.791277258567, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652224, Length=642, Percent_Identity=26.791277258567, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652222, Length=642, Percent_Identity=26.791277258567, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652220, Length=642, Percent_Identity=26.791277258567, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652218, Length=642, Percent_Identity=26.791277258567, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652212, Length=640, Percent_Identity=26.09375, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652210, Length=640, Percent_Identity=26.09375, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652214, Length=640, Percent_Identity=26.09375, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921944, Length=640, Percent_Identity=26.09375, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652216, Length=640, Percent_Identity=26.09375, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 65978; Mature: 65847

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEE
NEDAFTMMDRFRAIVGKDANLQTLARGVNTVTLDTGNSELIDLHAKLFAKHGTTTIRNFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCH
ALNDVNNLKFSGECIVKHGLKHEITITLMDLPSNCKGAHDVPFYEKILKEILTAEIPFHS
HHCCCCCCEECCCCCEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
ICFKDASGTSNPNKIYKTIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVDGIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVN
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEC
PLIPFSPMPGGALTANTQMMRDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQA
CCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FNNVMFGPWKKIAEGYGKMVLGYFGKTPVTPDANIVELASKQLNLEPTTELAINIADKDE
HCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
SKSIAYTKTLLEKEGIETSEENIFIAAACKEKGIAFLKGEAKVNIRKLASMPKPMSVDEN
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCEEHHHHHCCCCCCCCCCC
KFTVAVNGNKYHVEVSYGFDKDVNVKSVKKVEENKNIISSNSTSSVDAENEILAGISGNV
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCCCCE
FKIYVNEGEEVKSGQAIMVLEAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
EEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure 
AKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEE
NEDAFTMMDRFRAIVGKDANLQTLARGVNTVTLDTGNSELIDLHAKLFAKHGTTTIRNFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCH
ALNDVNNLKFSGECIVKHGLKHEITITLMDLPSNCKGAHDVPFYEKILKEILTAEIPFHS
HHCCCCCCEECCCCCEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
ICFKDASGTSNPNKIYKTIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVDGIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVN
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEC
PLIPFSPMPGGALTANTQMMRDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQA
CCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FNNVMFGPWKKIAEGYGKMVLGYFGKTPVTPDANIVELASKQLNLEPTTELAINIADKDE
HCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
SKSIAYTKTLLEKEGIETSEENIFIAAACKEKGIAFLKGEAKVNIRKLASMPKPMSVDEN
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCEEHHHHHCCCCCCCCCCC
KFTVAVNGNKYHVEVSYGFDKDVNVKSVKKVEENKNIISSNSTSSVDAENEILAGISGNV
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCCCCE
FKIYVNEGEEVKSGQAIMVLEAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
EEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]