Click here to switch to the map view.

The map label for this gene is hipO [H]

Identifier: 159185354

GI number: 159185354

Start: 2727949

End: 2729088

Strand: Direct

Name: hipO [H]

Synonym: Atu2732

Alternate gene names: 159185354

Gene position: 2727949-2729088 (Clockwise)

Preceding gene: 159185351

Following gene: 15889983

Centisome position: 96.0

GC content: 61.23

Gene sequence:

>1140_bases
ATGCCAGACGTGGTGGCGATCCGCCGCCATCTGCACCGCCACCCCGAGATCGGTCTTTCGGAATTCAAGACGTCGGATTT
CATTGCCGAACAGCTGGTGGAAATGGGCTACGAGGTGACGCGCGGGCTTGCCGGGACCGGCATCGTCGCGACGCTCCGAA
ACGGTGACAGCACCCGCGCGCTCGGCATTCGCGCCGACATCGACGCACTGCCGATCCACGAGGAAACCGGGGCGGACTAT
GCCAGCACCCATCAGGGCGTCATGCACGCCTGCGGTCACGATGGCCACACGGCCATGCTGCTCGGAGCGGCGAAGATCAT
CGCCGAACGCAGGAATTTCGATGGCACCCTGCATCTGATTTTCCAGCCGGCCGAGGAAAATTTCGGCGGCGCCCGCATCA
TGATCGAAGACGGCCTGTTCGAGCGCTTCCCGTGCGATGCAGTCTTTGCCCTGCATAATGATCCCGGCCTGCCCTTCGGA
CAATTCGTGCTGCGCGACGGGCCGATCCTTGCCGCCGTCGACGAATGCAAAATCACTGTCAGAGGTTATGGCGGCCACGG
CGCCGAACCGCAGGATGCGGCCGATCCCATCGTTGCCGGCGCGAGCATCATCATGGCGTTGCAGACGGTGGTGTCCCGCA
ATATCCACCCGCAACTGTCGGCGGTCGTGACCGTTGGCGCATTCCATGCTGGCATGGCGAGCAACGTCATTCCCGAAACG
GCAGAGATGTTGCTGACCATCCGCTCCTTCGATCCGGGCGTGCGCGACGAGCTGGAAAAGCGTATCCGGGCCATCGCCGA
AGGCCAGGCAGCAAGCTACGGCATGAGCGTCACCATCGATTACGAGCGCGGTTATAACGCAACCGTCAATCACAAGGCGG
AGACGGATTATGTCGCCGATCTCGCCCGTCGTTTTGCCGGACCGGAGAAGGTTCTGGAAATGAAGCGTCCATCAATGGGC
GCGGAAGACTTCGCCTATATGCTGGAAAAGCGGCCGGGCTGCTATTTCTTCCTCGGCACCGCGCGCACCGATAACGATCC
GCCACTGCACCATCCGAAATTCGATTTCAACGACGAGATTCTACCGATCGGAACCACCTTCTGGGTGGATCTGGCGGAGG
ACTATCTGAAGGCGCGATAA

Upstream 100 bases:

>100_bases
CTGATATGTCCGATGGGAAATACACCGAGACAGAACGGCCTAGCCGGGGACTTCGAAAATGACCAGACAGACCGCAAACA
TCGCCTCCTTTGCCAACCAT

Downstream 100 bases:

>100_bases
ACATGAAAAGGCCGGTCGGAGGAGCGACCGGCCTTTATTCTCAACTTGCCTGAAAACTAGGGAGGATCAGGGAACGAGAC
CGAATATGACAGCAGCAACG

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRALGIRADIDALPIHEETGADY
ASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLIFQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFG
QFVLRDGPILAAVDECKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET
AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVADLARRFAGPEKVLEMKRPSMG
AEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEILPIGTTFWVDLAEDYLKAR

Sequences:

>Translated_379_residues
MPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRALGIRADIDALPIHEETGADY
ASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLIFQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFG
QFVLRDGPILAAVDECKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET
AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVADLARRFAGPEKVLEMKRPSMG
AEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEILPIGTTFWVDLAEDYLKAR
>Mature_378_residues
PDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRALGIRADIDALPIHEETGADYA
STHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLIFQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQ
FVLRDGPILAAVDECKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPETA
EMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVADLARRFAGPEKVLEMKRPSMGA
EDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEILPIGTTFWVDLAEDYLKAR

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=415, Percent_Identity=27.9518072289157, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41456; Mature: 41325

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCEE
LGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLI
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
FQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAAVDECKITV
EEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHEEECCCEEEEEECEEEEE
RGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVAD
HHHEEEHCCCCCCHHHHHHHHHHHHHCCCCHHCCEEEEEEECCCCCCEECCCCCHHHHHH
LARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEI
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCE
LPIGTTFWVDLAEDYLKAR
EECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCEE
LGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLI
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
FQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAAVDECKITV
EEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHEEECCCEEEEEECEEEEE
RGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVAD
HHHEEEHCCCCCCHHHHHHHHHHHHHCCCCHHCCEEEEEEECCCCCCEECCCCCHHHHHH
LARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEI
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCE
LPIGTTFWVDLAEDYLKAR
EECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]