Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 15889190

GI number: 15889190

Start: 1870336

End: 1871121

Strand: Direct

Name: cobS [H]

Synonym: Atu1891

Alternate gene names: 15889190

Gene position: 1870336-1871121 (Clockwise)

Preceding gene: 15889187

Following gene: 17935782

Centisome position: 65.82

GC content: 63.87

Gene sequence:

>786_bases
ATGAAGGCCGGGGATTTTATAACAGATGTCATGCATTCCGTAGCCTTTCTCAGCCGCCTGCCGGTTCCCTCGCGGTTTTT
CGGTGAAGGTGACGGCGCCTCGATGCGCCGCACCGCCCGCGCCTTTCCCGCCGCCGGCCTGCTGATCGCCCTACCCGCCG
CTTTTCTGGTGGTGATTTTCGCCACCTTCGATGCATCGCCGCAGCTCACGGGCTGGCTCGCCATCGGCCTGACGGCGCTC
ATCACGGGCGCATTGCATGAGGACGGGCTGGCCGACATGGCCGACGGTTTCGGCAGCGGCAAGGACAAGGCCCGCATGCT
TGAGATCATGAAGGACAGCCGCATCGGCAGCTATGGCACCATCGCCATGGTTCTTTCCTTCGCGCTGCGCGCTACGGCGC
TCGCATCGCTGATCGAGACCCTGCCCGGAAAAACTGCCGCCGCCTGCCTGATTGCCACGCTTGTCATGAGCCGCGCCCTG
ATGGTGTGGCACTGGCAGGCCCTGCCCGCTGCCAAGACATCAGGCATCGCCGCCGGCGCGGGCCAGCCCGGAGAAAGCGA
CCGCAACATCGCACTGGTGACGGGTTTACTGGTCTTCATCCTCTTCACGCTGCACGCTTTGCCCATCCTGTCGATTGCAC
TTGTGATGGCGGCAGCCATTTTGGCGACGGTGCTGTTCGGCCGGCTCTGCGACAGGAAGATCGGCGGCCACACCGGCGAT
ACCATCGGCGCCTGCCAGCAGATCACGGAGATCGTTACTCTCGTCGCACTTGCTCTTGCGGCATGA

Upstream 100 bases:

>100_bases
GGAACTTGTGTTTTCCGGCAAATCGGTCTTTGTTGCGCCTCTCATAATCGCGGAAACGCCGCGGAACAACCGGAAATGCA
TTGACTGCAGCGGAGAGAGC

Downstream 100 bases:

>100_bases
CGGCCTGATATAGATCATCATGGAAACACCCTGCATCCATATCTGTCGCCTCGATGTGACCCATAGCCTCTGCATCGGCT
GCGGACGCACGCTGGATGAA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKAGDFITDVMHSVAFLSRLPVPSRFFGEGDGASMRRTARAFPAAGLLIALPAAFLVVIFATFDASPQLTGWLAIGLTAL
ITGALHEDGLADMADGFGSGKDKARMLEIMKDSRIGSYGTIAMVLSFALRATALASLIETLPGKTAAACLIATLVMSRAL
MVWHWQALPAAKTSGIAAGAGQPGESDRNIALVTGLLVFILFTLHALPILSIALVMAAAILATVLFGRLCDRKIGGHTGD
TIGACQQITEIVTLVALALAA

Sequences:

>Translated_261_residues
MKAGDFITDVMHSVAFLSRLPVPSRFFGEGDGASMRRTARAFPAAGLLIALPAAFLVVIFATFDASPQLTGWLAIGLTAL
ITGALHEDGLADMADGFGSGKDKARMLEIMKDSRIGSYGTIAMVLSFALRATALASLIETLPGKTAAACLIATLVMSRAL
MVWHWQALPAAKTSGIAAGAGQPGESDRNIALVTGLLVFILFTLHALPILSIALVMAAAILATVLFGRLCDRKIGGHTGD
TIGACQQITEIVTLVALALAA
>Mature_261_residues
MKAGDFITDVMHSVAFLSRLPVPSRFFGEGDGASMRRTARAFPAAGLLIALPAAFLVVIFATFDASPQLTGWLAIGLTAL
ITGALHEDGLADMADGFGSGKDKARMLEIMKDSRIGSYGTIAMVLSFALRATALASLIETLPGKTAAACLIATLVMSRAL
MVWHWQALPAAKTSGIAAGAGQPGESDRNIALVTGLLVFILFTLHALPILSIALVMAAAILATVLFGRLCDRKIGGHTGD
TIGACQQITEIVTLVALALAA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=32.258064516129, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27077; Mature: 27077

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGDFITDVMHSVAFLSRLPVPSRFFGEGDGASMRRTARAFPAAGLLIALPAAFLVVIF
CCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATFDASPQLTGWLAIGLTALITGALHEDGLADMADGFGSGKDKARMLEIMKDSRIGSYGT
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHH
IAMVLSFALRATALASLIETLPGKTAAACLIATLVMSRALMVWHWQALPAAKTSGIAAGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
GQPGESDRNIALVTGLLVFILFTLHALPILSIALVMAAAILATVLFGRLCDRKIGGHTGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TIGACQQITEIVTLVALALAA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAGDFITDVMHSVAFLSRLPVPSRFFGEGDGASMRRTARAFPAAGLLIALPAAFLVVIF
CCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATFDASPQLTGWLAIGLTALITGALHEDGLADMADGFGSGKDKARMLEIMKDSRIGSYGT
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHH
IAMVLSFALRATALASLIETLPGKTAAACLIATLVMSRALMVWHWQALPAAKTSGIAAGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
GQPGESDRNIALVTGLLVFILFTLHALPILSIALVMAAAILATVLFGRLCDRKIGGHTGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TIGACQQITEIVTLVALALAA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA