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The map label for this gene is nreB [H]

Identifier: 39995363

GI number: 39995363

Start: 260497

End: 262989

Strand: Direct

Name: nreB [H]

Synonym: GSU0253

Alternate gene names: 39995363

Gene position: 260497-262989 (Clockwise)

Preceding gene: 39995362

Following gene: 39995364

Centisome position: 6.83

GC content: 62.33

Gene sequence:

>2493_bases
GTGATCCATCGGCACCTTCAACATTCGCCGTTTCCCGTTCTGCTGGTAGAACCGCAGGGACGCATTGTGCAAGCCAGCCC
GGCAAGTCTCGAATTCCTGGGCATAGGATCGCGTCACTCCGCCCCCAGCGCTCTGGCCGACATCATGCATGCGGAACAGT
GCGAGGCCGTGCTTCGCGCCGGAGCCGCCTTGACCGACACCGGGTTTTGGGAAGGAGAATGTTCTTTGATCGGGCGGGAT
GGGCAGCCCGTGCGGGCGTTCCTCCGGGTGGCCAGGGCTGGTGATGACCGCCTGGTGATGTACCTTCGGGACTCCGCCGG
GCGCAGTCGCTCCGAAGAGTTGCTGCGCATCCGTTTGCAGCTGTCGGAACGTGCCCGGTGCGAAAGCCTCGACGAGTTAC
TTCGGTCAGCCCTGGATGCCGCTGAACGGCTGACCGACAGCAGTATCGGTTACCTCCATGTTGTTGCCCCGGACCGGGAG
CAGGCTTCCCTGCAGGCGTGGTCGAGCAATACCCTGGCCACCATGTGCCGGGTGAGAGGCGAAAAAGAGCACCATCCGAC
CAGACGGGCCGGGATCTGGGGTGATTGCTGTCGCGAACGTACTGCCATGATCCATAACGATTACGCCGGGCTTCCACATA
AAAAGGAACTGCCCGAGGGACACCCCCCCATTGTGCGGGTGATGGGTGTGCCGATCATTCATGGCGACAAGGTGCTCGCC
ATTATCGGCGTGGGCAACAAGGCGACGGAGTACATCCGTGAAGATGCGGATGCGCTGCATGAGCTCGCCTCGATGATCAT
GGACCTGGTGGAGCGCAAGCAGGCTGAAGCGCAGTTGCTCGCAAGCGAAAAGCGCTTCAGGACGGTCTTTGAGCAGGCGG
CAGTCGGGGTGGCCCTGGTTGATCCGCTGACGGGCCGGTTCGTCAGGGTCAATGCGAAATACGGCGACATCGTCGGCTAC
TCTCCCGAGACAATGCTGACGATGGGGTTCCAGGAGCTCACCCACCCCGACGATCTGGCGGTCGACCTGGAGTGCATCTC
CCGGCTCTTGAAGGGGGAAATCCGTACCTTTACGAGAGAGAAGCGTTACCTGCACCGGGACGGCTGCAACGTGTGGGTCA
GACTGACCGTCTCTCCCCTGTGGGCGGACGGCGAGGAGCCGAGCTTCAACATCGCCATCGTGGAAGACATAACCGCCCGC
AAGGCGGCGGAGGCGGCCCTCCGGGAAGCGAAGGAACAGCTGGAGCTTCGCGTCAGGGAAAGGACTGCGGATCTGGAACA
GGCCAACCGGGCCTTGCAGGAGGAGGTAGCCGCACGCAGCCAGGCCGAGGAATCTCTTCGTGCAAGCGAGTCAAGGTTCA
GGCGTCTCGTCCAGATGGCGCCGCTGCCACTGTGCTTCGCGGATGCGAGCGGCACCTTGCGGTACGTAAACGACCGGTTC
ACGCGGCTGTTCGGCTATGCGCCGGAAGACATCCCGACGCAGGAGGAGTGGTGGAGTCGGGTTTGCACCGATGGGCGCTG
CCGAGGGGAGACGGCCGATGCCTGGCAGGCGTCCGTGCAAAGAGGCCCGAACGGTGCGAGTGATATCGAACCCGCTGAAT
TCCGGGTCGCGTGCAAGAACGGTGATATTCGTCTGGTGGAAGTTTCCGGGATAACGATTGAGGGCACGGTCCTGGCGACA
TTCATCGATATCACCGAGCGCCGGTTGGCGGAGGATGAGCTTCATCGCCACAGGGAACTGCTCGAGGAGCTGGTTCACCA
GCGGACTGCGGAACTGAAACTGCGGAACATGGAGCTGGCCTCCGAGATTGCCGAACGCCGGCGGGCCGAGGCAGCCCTCC
GCTGCTACACCCGGCGTCTCACCGAGATGGAAGAGGACCTGAGGAAGAAGCTCGCCGCCGAACTGCACGACGAGATCGGG
AGGGATCTGACCGCCCTCAATCTCAACTTTTCCGTTATCGGCAACCGTCTCGTGCAGGGCCCGGACGGGTGCCTCAAGGC
GAGGATCGAAGACACGGAACGGCTTATCGAAGACATAAGCCGCACGGTCCGGGGCCTAACTGCCAAGCTGCGGCCGCCCG
TGCTGGACGACTACGGTCTGCCGGCAGCGCTACGCTGGCATGCTGATCTTTTTTCACGGCGGACCGGGATTGAGGTGACG
GTACGGGGGGCCGACAGCGTTCCCCGGATGCCTGCGGAAAAGGAAATAGCGCTCTTTCGTATCGTTCAGGAGGCCCTCAC
GAATTGTTCGAAACATGCAAACGCAGGGAACGTGAAGGTCTCCCTGGATCACGGCGATGGGCGGGTGCGGCTTTCGGTTG
TGGACGACGGTATTGGATTTGCACCTGAAAGTGCCTCGCCGACACCGGCGGGAAGCGGCTGGGGGCTGACAATCATGCGC
GAGCGGGCTGAACTGATCGGCGGGACGTTCCGGCTGAATACCGCGCCGGGCAAAGGTACGGTACTGACTGTTGAAATTGA
CGAGGGTTCGTAG

Upstream 100 bases:

>100_bases
CGGCGATTTGCTTACGGCATTAATAGACTTAGCTGAGTTCAAGGGAACAGTCGAACCTCGGGCTCGAAGTGTCTGATCTG
TATTTGCAGGAGTTTCGGAA

Downstream 100 bases:

>100_bases
ATGGTGATCAAGGTTCTCATTGCCGATGACCATGCCGTCGTAAGGGACGGACTGACCATGATTCTCGAATCCCAGAGCGA
TATTGCCGTGATCGGTCAGG

Product: sensory box histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 830; Mature: 830

Protein sequence:

>830_residues
MIHRHLQHSPFPVLLVEPQGRIVQASPASLEFLGIGSRHSAPSALADIMHAEQCEAVLRAGAALTDTGFWEGECSLIGRD
GQPVRAFLRVARAGDDRLVMYLRDSAGRSRSEELLRIRLQLSERARCESLDELLRSALDAAERLTDSSIGYLHVVAPDRE
QASLQAWSSNTLATMCRVRGEKEHHPTRRAGIWGDCCRERTAMIHNDYAGLPHKKELPEGHPPIVRVMGVPIIHGDKVLA
IIGVGNKATEYIREDADALHELASMIMDLVERKQAEAQLLASEKRFRTVFEQAAVGVALVDPLTGRFVRVNAKYGDIVGY
SPETMLTMGFQELTHPDDLAVDLECISRLLKGEIRTFTREKRYLHRDGCNVWVRLTVSPLWADGEEPSFNIAIVEDITAR
KAAEAALREAKEQLELRVRERTADLEQANRALQEEVAARSQAEESLRASESRFRRLVQMAPLPLCFADASGTLRYVNDRF
TRLFGYAPEDIPTQEEWWSRVCTDGRCRGETADAWQASVQRGPNGASDIEPAEFRVACKNGDIRLVEVSGITIEGTVLAT
FIDITERRLAEDELHRHRELLEELVHQRTAELKLRNMELASEIAERRRAEAALRCYTRRLTEMEEDLRKKLAAELHDEIG
RDLTALNLNFSVIGNRLVQGPDGCLKARIEDTERLIEDISRTVRGLTAKLRPPVLDDYGLPAALRWHADLFSRRTGIEVT
VRGADSVPRMPAEKEIALFRIVQEALTNCSKHANAGNVKVSLDHGDGRVRLSVVDDGIGFAPESASPTPAGSGWGLTIMR
ERAELIGGTFRLNTAPGKGTVLTVEIDEGS

Sequences:

>Translated_830_residues
MIHRHLQHSPFPVLLVEPQGRIVQASPASLEFLGIGSRHSAPSALADIMHAEQCEAVLRAGAALTDTGFWEGECSLIGRD
GQPVRAFLRVARAGDDRLVMYLRDSAGRSRSEELLRIRLQLSERARCESLDELLRSALDAAERLTDSSIGYLHVVAPDRE
QASLQAWSSNTLATMCRVRGEKEHHPTRRAGIWGDCCRERTAMIHNDYAGLPHKKELPEGHPPIVRVMGVPIIHGDKVLA
IIGVGNKATEYIREDADALHELASMIMDLVERKQAEAQLLASEKRFRTVFEQAAVGVALVDPLTGRFVRVNAKYGDIVGY
SPETMLTMGFQELTHPDDLAVDLECISRLLKGEIRTFTREKRYLHRDGCNVWVRLTVSPLWADGEEPSFNIAIVEDITAR
KAAEAALREAKEQLELRVRERTADLEQANRALQEEVAARSQAEESLRASESRFRRLVQMAPLPLCFADASGTLRYVNDRF
TRLFGYAPEDIPTQEEWWSRVCTDGRCRGETADAWQASVQRGPNGASDIEPAEFRVACKNGDIRLVEVSGITIEGTVLAT
FIDITERRLAEDELHRHRELLEELVHQRTAELKLRNMELASEIAERRRAEAALRCYTRRLTEMEEDLRKKLAAELHDEIG
RDLTALNLNFSVIGNRLVQGPDGCLKARIEDTERLIEDISRTVRGLTAKLRPPVLDDYGLPAALRWHADLFSRRTGIEVT
VRGADSVPRMPAEKEIALFRIVQEALTNCSKHANAGNVKVSLDHGDGRVRLSVVDDGIGFAPESASPTPAGSGWGLTIMR
ERAELIGGTFRLNTAPGKGTVLTVEIDEGS
>Mature_830_residues
MIHRHLQHSPFPVLLVEPQGRIVQASPASLEFLGIGSRHSAPSALADIMHAEQCEAVLRAGAALTDTGFWEGECSLIGRD
GQPVRAFLRVARAGDDRLVMYLRDSAGRSRSEELLRIRLQLSERARCESLDELLRSALDAAERLTDSSIGYLHVVAPDRE
QASLQAWSSNTLATMCRVRGEKEHHPTRRAGIWGDCCRERTAMIHNDYAGLPHKKELPEGHPPIVRVMGVPIIHGDKVLA
IIGVGNKATEYIREDADALHELASMIMDLVERKQAEAQLLASEKRFRTVFEQAAVGVALVDPLTGRFVRVNAKYGDIVGY
SPETMLTMGFQELTHPDDLAVDLECISRLLKGEIRTFTREKRYLHRDGCNVWVRLTVSPLWADGEEPSFNIAIVEDITAR
KAAEAALREAKEQLELRVRERTADLEQANRALQEEVAARSQAEESLRASESRFRRLVQMAPLPLCFADASGTLRYVNDRF
TRLFGYAPEDIPTQEEWWSRVCTDGRCRGETADAWQASVQRGPNGASDIEPAEFRVACKNGDIRLVEVSGITIEGTVLAT
FIDITERRLAEDELHRHRELLEELVHQRTAELKLRNMELASEIAERRRAEAALRCYTRRLTEMEEDLRKKLAAELHDEIG
RDLTALNLNFSVIGNRLVQGPDGCLKARIEDTERLIEDISRTVRGLTAKLRPPVLDDYGLPAALRWHADLFSRRTGIEVT
VRGADSVPRMPAEKEIALFRIVQEALTNCSKHANAGNVKVSLDHGDGRVRLSVVDDGIGFAPESASPTPAGSGWGLTIMR
ERAELIGGTFRLNTAPGKGTVLTVEIDEGS

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=305, Percent_Identity=28.5245901639344, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1788812, Length=212, Percent_Identity=29.7169811320755, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787474, Length=438, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082311, Length=242, Percent_Identity=33.0578512396694, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 92440; Mature: 92440

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHRHLQHSPFPVLLVEPQGRIVQASPASLEFLGIGSRHSAPSALADIMHAEQCEAVLRA
CCCCCCCCCCCCEEEECCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
GAALTDTGFWEGECSLIGRDGQPVRAFLRVARAGDDRLVMYLRDSAGRSRSEELLRIRLQ
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
LSERARCESLDELLRSALDAAERLTDSSIGYLHVVAPDREQASLQAWSSNTLATMCRVRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCC
EKEHHPTRRAGIWGDCCRERTAMIHNDYAGLPHKKELPEGHPPIVRVMGVPIIHGDKVLA
CCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCEEE
IIGVGNKATEYIREDADALHELASMIMDLVERKQAEAQLLASEKRFRTVFEQAAVGVALV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DPLTGRFVRVNAKYGDIVGYSPETMLTMGFQELTHPDDLAVDLECISRLLKGEIRTFTRE
CCCCCCEEEEECCCCCEECCCCCHHHHHCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
KRYLHRDGCNVWVRLTVSPLWADGEEPSFNIAIVEDITARKAAEAALREAKEQLELRVRE
HHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RTADLEQANRALQEEVAARSQAEESLRASESRFRRLVQMAPLPLCFADASGTLRYVNDRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHH
TRLFGYAPEDIPTQEEWWSRVCTDGRCRGETADAWQASVQRGPNGASDIEPAEFRVACKN
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECC
GDIRLVEVSGITIEGTVLATFIDITERRLAEDELHRHRELLEELVHQRTAELKLRNMELA
CCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIAERRRAEAALRCYTRRLTEMEEDLRKKLAAELHDEIGRDLTALNLNFSVIGNRLVQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCHHHCC
PDGCLKARIEDTERLIEDISRTVRGLTAKLRPPVLDDYGLPAALRWHADLFSRRTGIEVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VRGADSVPRMPAEKEIALFRIVQEALTNCSKHANAGNVKVSLDHGDGRVRLSVVDDGIGF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCC
APESASPTPAGSGWGLTIMRERAELIGGTFRLNTAPGKGTVLTVEIDEGS
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCC
>Mature Secondary Structure
MIHRHLQHSPFPVLLVEPQGRIVQASPASLEFLGIGSRHSAPSALADIMHAEQCEAVLRA
CCCCCCCCCCCCEEEECCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
GAALTDTGFWEGECSLIGRDGQPVRAFLRVARAGDDRLVMYLRDSAGRSRSEELLRIRLQ
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
LSERARCESLDELLRSALDAAERLTDSSIGYLHVVAPDREQASLQAWSSNTLATMCRVRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCC
EKEHHPTRRAGIWGDCCRERTAMIHNDYAGLPHKKELPEGHPPIVRVMGVPIIHGDKVLA
CCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCEEE
IIGVGNKATEYIREDADALHELASMIMDLVERKQAEAQLLASEKRFRTVFEQAAVGVALV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DPLTGRFVRVNAKYGDIVGYSPETMLTMGFQELTHPDDLAVDLECISRLLKGEIRTFTRE
CCCCCCEEEEECCCCCEECCCCCHHHHHCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
KRYLHRDGCNVWVRLTVSPLWADGEEPSFNIAIVEDITARKAAEAALREAKEQLELRVRE
HHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RTADLEQANRALQEEVAARSQAEESLRASESRFRRLVQMAPLPLCFADASGTLRYVNDRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHH
TRLFGYAPEDIPTQEEWWSRVCTDGRCRGETADAWQASVQRGPNGASDIEPAEFRVACKN
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECC
GDIRLVEVSGITIEGTVLATFIDITERRLAEDELHRHRELLEELVHQRTAELKLRNMELA
CCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIAERRRAEAALRCYTRRLTEMEEDLRKKLAAELHDEIGRDLTALNLNFSVIGNRLVQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCHHHCC
PDGCLKARIEDTERLIEDISRTVRGLTAKLRPPVLDDYGLPAALRWHADLFSRRTGIEVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VRGADSVPRMPAEKEIALFRIVQEALTNCSKHANAGNVKVSLDHGDGRVRLSVVDDGIGF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCC
APESASPTPAGSGWGLTIMRERAELIGGTFRLNTAPGKGTVLTVEIDEGS
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]