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The map label for this gene is trmB

Identifier: 38234724

GI number: 38234724

Start: 2258718

End: 2259497

Strand: Direct

Name: trmB

Synonym: DIP2181

Alternate gene names: 38234724

Gene position: 2258718-2259497 (Clockwise)

Preceding gene: 38234722

Following gene: 38234725

Centisome position: 90.76

GC content: 59.87

Gene sequence:

>780_bases
ATGTCTAATACTGATAATTCTGATAAGAACACCAAGCCCACCGGCTATCGTCCACCACAAACGGACTTCAACACCGAGTT
CGGCAACAACCTCGATTACCCACGCCTTGGCTCCGTCAGCTTCCGCCGCGGCACCCTCACCGACAACCAAGAATCCCTCT
GGGACGCCAACTGGCCCATCCTAGGCAAGGACCTGACCGACGCCGAGGACCAGCGTATCGACGTCGCCGAGTGGTTCGGT
CGCTGCGGCCACAAGACCATCCTCGAGATCGGTTCCGGCACCGGCACCTCCACGGCAGCCATGGCACCACTAGAAGCCGA
CACCAACATCATCGCCGTCGAGCTGTACAAGCCAGGTCTTGCCAAGCTGCTCGGCGCAGTCGTGCGCGGCGACATCTCCA
ACGTGCGCATGATCCGCGGCGACGGCGTTGAAGTACTCACCCGCATGCTCCCCGAGGAAAGCCTCGACGGTGTGCGTATC
TACTTCCCAGACCCTTGGCCTAAAGCCCGCCACCACAAGCGCCGCATCATTCAGTCCGGCGTGCTCAACCTGATCGCGTC
ACGCCTCAAGCCAGGCGGCGTTCTGCACGTGGCAACCGATCATGCCGACTATGCCGAGTGGATTACCGAGCTCGTCAACG
TAGAGCCACAGTTGGAGTTCATGGGTTGGCCATGGGATGAGTGCCCACAGCTAACCGATCGCCAGGTGATCACCAAGTTT
GAAGGCAAGGGCTTGGATAAGGACCACACCATCACAGAGTTCCTGTGGAGGCGCAAGTGA

Upstream 100 bases:

>100_bases
AATACCCTACCCCCTCCCATGTCACTATTTTCACACCCGTCACATATTTATCACCTGTCTGATATTGAACTAATCACAAC
CGAGGGGCACAATAGTCGGG

Downstream 100 bases:

>100_bases
GCCGGCTGCTACTCGTATGGGATGCCCCCAACCTTGATATGGGTCTAGGAGCGCTGCTCGGCGGTCGCCCCACCACTGCT
CATCGTCCGCGTTTCGACGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSNTDNSDKNTKPTGYRPPQTDFNTEFGNNLDYPRLGSVSFRRGTLTDNQESLWDANWPILGKDLTDAEDQRIDVAEWFG
RCGHKTILEIGSGTGTSTAAMAPLEADTNIIAVELYKPGLAKLLGAVVRGDISNVRMIRGDGVEVLTRMLPEESLDGVRI
YFPDPWPKARHHKRRIIQSGVLNLIASRLKPGGVLHVATDHADYAEWITELVNVEPQLEFMGWPWDECPQLTDRQVITKF
EGKGLDKDHTITEFLWRRK

Sequences:

>Translated_259_residues
MSNTDNSDKNTKPTGYRPPQTDFNTEFGNNLDYPRLGSVSFRRGTLTDNQESLWDANWPILGKDLTDAEDQRIDVAEWFG
RCGHKTILEIGSGTGTSTAAMAPLEADTNIIAVELYKPGLAKLLGAVVRGDISNVRMIRGDGVEVLTRMLPEESLDGVRI
YFPDPWPKARHHKRRIIQSGVLNLIASRLKPGGVLHVATDHADYAEWITELVNVEPQLEFMGWPWDECPQLTDRQVITKF
EGKGLDKDHTITEFLWRRK
>Mature_258_residues
SNTDNSDKNTKPTGYRPPQTDFNTEFGNNLDYPRLGSVSFRRGTLTDNQESLWDANWPILGKDLTDAEDQRIDVAEWFGR
CGHKTILEIGSGTGTSTAAMAPLEADTNIIAVELYKPGLAKLLGAVVRGDISNVRMIRGDGVEVLTRMLPEESLDGVRIY
FPDPWPKARHHKRRIIQSGVLNLIASRLKPGGVLHVATDHADYAEWITELVNVEPQLEFMGWPWDECPQLTDRQVITKFE
GKGLDKDHTITEFLWRRK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=224, Percent_Identity=36.6071428571429, Blast_Score=144, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320000, Length=157, Percent_Identity=27.3885350318471, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CORDI (Q6NET4)

Other databases:

- EMBL:   BX248360
- RefSeq:   NP_940491.1
- ProteinModelPortal:   Q6NET4
- GeneID:   2648927
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2181
- NMPDR:   fig|257309.1.peg.2082
- HOGENOM:   HBG322782
- OMA:   NIRLIRG
- PhylomeDB:   Q6NET4
- ProtClustDB:   PRK00121
- BioCyc:   CDIP257309:DIP2181-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29200; Mature: 29069

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 114-114 BINDING 141-141 BINDING 164-164 BINDING 168-168 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTDNSDKNTKPTGYRPPQTDFNTEFGNNLDYPRLGSVSFRRGTLTDNQESLWDANWPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHCCCCCC
LGKDLTDAEDQRIDVAEWFGRCGHKTILEIGSGTGTSTAAMAPLEADTNIIAVELYKPGL
CCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCCCCCHHEECCCCCCCCEEEEEECCCCH
AKLLGAVVRGDISNVRMIRGDGVEVLTRMLPEESLDGVRIYFPDPWPKARHHKRRIIQSG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VLNLIASRLKPGGVLHVATDHADYAEWITELVNVEPQLEFMGWPWDECPQLTDRQVITKF
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCHHHHHHH
EGKGLDKDHTITEFLWRRK
CCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SNTDNSDKNTKPTGYRPPQTDFNTEFGNNLDYPRLGSVSFRRGTLTDNQESLWDANWPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHCCCCCC
LGKDLTDAEDQRIDVAEWFGRCGHKTILEIGSGTGTSTAAMAPLEADTNIIAVELYKPGL
CCCCCCCCCCCEECHHHHHHHCCCCEEEEECCCCCCCHHEECCCCCCCCEEEEEECCCCH
AKLLGAVVRGDISNVRMIRGDGVEVLTRMLPEESLDGVRIYFPDPWPKARHHKRRIIQSG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VLNLIASRLKPGGVLHVATDHADYAEWITELVNVEPQLEFMGWPWDECPQLTDRQVITKF
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCHHHHHHH
EGKGLDKDHTITEFLWRRK
CCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910