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The map label for this gene is pckG

Identifier: 38234723

GI number: 38234723

Start: 2256423

End: 2258261

Strand: Reverse

Name: pckG

Synonym: DIP2180

Alternate gene names: 38234723

Gene position: 2258261-2256423 (Counterclockwise)

Preceding gene: 38234729

Following gene: 38234721

Centisome position: 90.74

GC content: 59.22

Gene sequence:

>1839_bases
ATGTCCACCGTGTCCATTAAGGGCCTTGAGGGTGAAGCACCCACCAAAAATGAAGTACTGCTGAATTGGATTGCGGAAAA
CGTCGAGCTTTTCCAGCCGGACCGAGTTGTTTTTGCTGACGGATCCCAAGAGGAATGGGACCGCCTGACAACTCAATTGG
TAGAGTCCGGTACTCTCATCCGTCTCAATGAAGAGAAGCGCCCCAATAGCTTCTTGGCTCGTTCCAACCCAGCCGACGTT
GCCCGGGTGGAGTCCCGTACCTTCATCTGTACCCCAACCCCAGAAGGTGCAGGTCCTACCAACCACTGGGCAGACCCAGC
GGAAATGAAAGCCGAGCTCACCGAGCGTTTCCGTGGCTCCATGAAGGGCCGCACCATGTACGTCGTTCCTTTCTGCATGG
GCCCTATCAACGACCCAGCCCCCAAGCTAGGCGTACAGCTCACCGACTCCGAGTACGTTGTGCTCTCCATGCGCATCATG
ACCCGCATGGGCGCAGAAGCACTGGCCAAGATCGGCGACACCGGCTCCTTCGTTCCATGCCTGCACTCCGTGGGCGCACC
TCTGGAACCGGGCCAAGAAGATGTGGCATGGCCATGCAACGAGGAAAAATACATCACCCACTTCCCAGAAACCAAGGAAA
TCTGGTCCTACGGCTCCGGCTACGGCGGAAACGCCATCTTGGCTAAGAAGTGCTACGCCCTGCGTATCGCCTCCGTCATG
GCAAAGGAAGAGGGCTGGATGGCTGAGCACATGCTCATTCTGAAACTCACCTCCCCAGAAAAGAAGGTCTACTACATCGC
CGCTGCCTTCCCATCGGCATGTGGCAAGACCAACCTCGCTATGCTCCAGCCCACTATCGACGGCTGGTCCGCCGAAGTCG
TTGGCGACGACATCGCATGGCTGCGCTTCGGTGAAGACGGCCGCCTCTACGCCATCAACCCAGAAAACGGCTTCTTCGGT
GTTGCTCCTGGCACCAACTACTCCTCCAACCCCAACGCTATGCGCACCATGGAACCAGGCAACACCTTGTTCACCAACGT
TGCGCTTACCGACGACGGCGACATCTGGTGGGAAGACCTGGAGAACATGCCAGAGCACCTCACTGACTGGTTGGGCAACG
ACTGGACCCCAGAAAGCAGCCAGCCATCCAGCCACCCGAACTCCCGCTATTGCGTCCCGCTGGCACAGTGCCCAGTCGCC
GCAGAAGAGTACAACGACCCACAGGGCGTGCCGATCTCTGCAATCTTGTTCGGTGGCCGCCGCCCAGACACCGTCCCGCT
GGTATCCCAGGCGCACAACTGGGATCATGCCACCATGATCGGTGCCCTGCTGTCCTCCGGTCAGACCGCCGCCGCAGAAG
GCACCGTCGGCGCACTACGCCACGATCCCATGGCCATGCTGCCATTCATTGGCTACAACGCAGCGGATTACCTACAGCAT
TGGATTGACATGGGCAAGAAGGGCGGCGACAAGATGCCTGAGGTATTCTTGGTCAACTGGTTCCGCCGTGGCGACGACGG
ACGCTTCCTGTGGCCAGGCTTCGGCGACAACAGCCGCGTTCTCAAGTGGATCGTTGACCGCATTGAAGGCCGCGTCGAAG
CAGACGAAACCATCGCCGGCTACACCGCCCGCGTGGAAGACTTGGACCTCACCGGCCTAGACACCCCAATCGAGGACATC
CGCGAGGCACTGTCCGCTCCAGCCGCACAATGGAAGAGCGACCTTGCCGACAACGAGGAATACCTCACGTTCCTAGGCTC
TAAGGGCTCCGCGGTTCCGGCTGAGGTCCTCGAACAATTCGAGGCACTCAAAGCTCGCGTAGCAGCAGCTGAATCCTAA

Upstream 100 bases:

>100_bases
GTGTATCTCCCACTGCATTGTCATTTGCTGTTCATCTTCTGGCTACTGTGTGGGGTGGAAGAACTGTCAATTGACACGCG
AAACTATGTCAGGAGAAGCG

Downstream 100 bases:

>100_bases
AGCAGTACCCCCAAGGTGGCTTCCTTACGTAAGGAAGCCACCTTTTTGTATCACCAGCACTAGGTTGCTCACCAAAAACT
CCCGCAGCACCGGCACCCGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSTVSIKGLEGEAPTKNEVLLNWIAENVELFQPDRVVFADGSQEEWDRLTTQLVESGTLIRLNEEKRPNSFLARSNPADV
ARVESRTFICTPTPEGAGPTNHWADPAEMKAELTERFRGSMKGRTMYVVPFCMGPINDPAPKLGVQLTDSEYVVLSMRIM
TRMGAEALAKIGDTGSFVPCLHSVGAPLEPGQEDVAWPCNEEKYITHFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
AKEEGWMAEHMLILKLTSPEKKVYYIAAAFPSACGKTNLAMLQPTIDGWSAEVVGDDIAWLRFGEDGRLYAINPENGFFG
VAPGTNYSSNPNAMRTMEPGNTLFTNVALTDDGDIWWEDLENMPEHLTDWLGNDWTPESSQPSSHPNSRYCVPLAQCPVA
AEEYNDPQGVPISAILFGGRRPDTVPLVSQAHNWDHATMIGALLSSGQTAAAEGTVGALRHDPMAMLPFIGYNAADYLQH
WIDMGKKGGDKMPEVFLVNWFRRGDDGRFLWPGFGDNSRVLKWIVDRIEGRVEADETIAGYTARVEDLDLTGLDTPIEDI
REALSAPAAQWKSDLADNEEYLTFLGSKGSAVPAEVLEQFEALKARVAAAES

Sequences:

>Translated_612_residues
MSTVSIKGLEGEAPTKNEVLLNWIAENVELFQPDRVVFADGSQEEWDRLTTQLVESGTLIRLNEEKRPNSFLARSNPADV
ARVESRTFICTPTPEGAGPTNHWADPAEMKAELTERFRGSMKGRTMYVVPFCMGPINDPAPKLGVQLTDSEYVVLSMRIM
TRMGAEALAKIGDTGSFVPCLHSVGAPLEPGQEDVAWPCNEEKYITHFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
AKEEGWMAEHMLILKLTSPEKKVYYIAAAFPSACGKTNLAMLQPTIDGWSAEVVGDDIAWLRFGEDGRLYAINPENGFFG
VAPGTNYSSNPNAMRTMEPGNTLFTNVALTDDGDIWWEDLENMPEHLTDWLGNDWTPESSQPSSHPNSRYCVPLAQCPVA
AEEYNDPQGVPISAILFGGRRPDTVPLVSQAHNWDHATMIGALLSSGQTAAAEGTVGALRHDPMAMLPFIGYNAADYLQH
WIDMGKKGGDKMPEVFLVNWFRRGDDGRFLWPGFGDNSRVLKWIVDRIEGRVEADETIAGYTARVEDLDLTGLDTPIEDI
REALSAPAAQWKSDLADNEEYLTFLGSKGSAVPAEVLEQFEALKARVAAAES
>Mature_611_residues
STVSIKGLEGEAPTKNEVLLNWIAENVELFQPDRVVFADGSQEEWDRLTTQLVESGTLIRLNEEKRPNSFLARSNPADVA
RVESRTFICTPTPEGAGPTNHWADPAEMKAELTERFRGSMKGRTMYVVPFCMGPINDPAPKLGVQLTDSEYVVLSMRIMT
RMGAEALAKIGDTGSFVPCLHSVGAPLEPGQEDVAWPCNEEKYITHFPETKEIWSYGSGYGGNAILAKKCYALRIASVMA
KEEGWMAEHMLILKLTSPEKKVYYIAAAFPSACGKTNLAMLQPTIDGWSAEVVGDDIAWLRFGEDGRLYAINPENGFFGV
APGTNYSSNPNAMRTMEPGNTLFTNVALTDDGDIWWEDLENMPEHLTDWLGNDWTPESSQPSSHPNSRYCVPLAQCPVAA
EEYNDPQGVPISAILFGGRRPDTVPLVSQAHNWDHATMIGALLSSGQTAAAEGTVGALRHDPMAMLPFIGYNAADYLQHW
IDMGKKGGDKMPEVFLVNWFRRGDDGRFLWPGFGDNSRVLKWIVDRIEGRVEADETIAGYTARVEDLDLTGLDTPIEDIR
EALSAPAAQWKSDLADNEEYLTFLGSKGSAVPAEVLEQFEALKARVAAAES

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=616, Percent_Identity=50.8116883116883, Blast_Score=598, Evalue=1e-171,
Organism=Homo sapiens, GI187281517, Length=620, Percent_Identity=48.0645161290323, Blast_Score=589, Evalue=1e-168,
Organism=Homo sapiens, GI66346723, Length=386, Percent_Identity=53.6269430051813, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17555492, Length=627, Percent_Identity=48.4848484848485, Blast_Score=571, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI71989645, Length=602, Percent_Identity=49.6677740863787, Blast_Score=569, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989658, Length=601, Percent_Identity=49.4176372712146, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989653, Length=594, Percent_Identity=49.4949494949495, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=546, Percent_Identity=51.4652014652015, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=535, Percent_Identity=51.0280373831776, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71989649, Length=501, Percent_Identity=50.6986027944112, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI32564409, Length=371, Percent_Identity=49.0566037735849, Blast_Score=345, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17540956, Length=463, Percent_Identity=24.8380129589633, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655085, Length=598, Percent_Identity=51.1705685618729, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24655082, Length=613, Percent_Identity=49.9184339314845, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI161077226, Length=571, Percent_Identity=51.3134851138354, Blast_Score=560, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24655088, Length=503, Percent_Identity=51.8886679920477, Blast_Score=536, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CORDI (Q6NET5)

Other databases:

- EMBL:   BX248360
- RefSeq:   NP_940490.1
- ProteinModelPortal:   Q6NET5
- SMR:   Q6NET5
- GeneID:   2648926
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2180
- NMPDR:   fig|257309.1.peg.2081
- HOGENOM:   HBG484437
- OMA:   WILERVE
- ProtClustDB:   PRK04210
- BioCyc:   CDIP257309:DIP2180-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 67353; Mature: 67222

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 274-274 BINDING 82-82 BINDING 223-223 BINDING 230-230 BINDING 272-272 BINDING 389-389 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVSIKGLEGEAPTKNEVLLNWIAENVELFQPDRVVFADGSQEEWDRLTTQLVESGTLI
CCCEEEECCCCCCCCCHHHHHHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCEE
RLNEEKRPNSFLARSNPADVARVESRTFICTPTPEGAGPTNHWADPAEMKAELTERFRGS
EECCCCCCCCCCCCCCCCHHHEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
MKGRTMYVVPFCMGPINDPAPKLGVQLTDSEYVVLSMRIMTRMGAEALAKIGDTGSFVPC
CCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEHHHHHHHHCHHHHHHHCCCCCHHHH
LHSVGAPLEPGQEDVAWPCNEEKYITHFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKEEGWMAEHMLILKLTSPEKKVYYIAAAFPSACGKTNLAMLQPTIDGWSAEVVGDDIAW
HHCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEE
LRFGEDGRLYAINPENGFFGVAPGTNYSSNPNAMRTMEPGNTLFTNVALTDDGDIWWEDL
EEECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCHHHHH
ENMPEHLTDWLGNDWTPESSQPSSHPNSRYCVPLAQCPVAAEEYNDPQGVPISAILFGGR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHCCCCCCCEEEEEEECCC
RPDTVPLVSQAHNWDHATMIGALLSSGQTAAAEGTVGALRHDPMAMLPFIGYNAADYLQH
CCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHCCCCHHHHHHH
WIDMGKKGGDKMPEVFLVNWFRRGDDGRFLWPGFGDNSRVLKWIVDRIEGRVEADETIAG
HHHHCCCCCCCCCCEEEEEHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
YTARVEDLDLTGLDTPIEDIREALSAPAAQWKSDLADNEEYLTFLGSKGSAVPAEVLEQF
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
EALKARVAAAES
HHHHHHHHHCCC
>Mature Secondary Structure 
STVSIKGLEGEAPTKNEVLLNWIAENVELFQPDRVVFADGSQEEWDRLTTQLVESGTLI
CCEEEECCCCCCCCCHHHHHHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCEE
RLNEEKRPNSFLARSNPADVARVESRTFICTPTPEGAGPTNHWADPAEMKAELTERFRGS
EECCCCCCCCCCCCCCCCHHHEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
MKGRTMYVVPFCMGPINDPAPKLGVQLTDSEYVVLSMRIMTRMGAEALAKIGDTGSFVPC
CCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEHHHHHHHHCHHHHHHHCCCCCHHHH
LHSVGAPLEPGQEDVAWPCNEEKYITHFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKEEGWMAEHMLILKLTSPEKKVYYIAAAFPSACGKTNLAMLQPTIDGWSAEVVGDDIAW
HHCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEE
LRFGEDGRLYAINPENGFFGVAPGTNYSSNPNAMRTMEPGNTLFTNVALTDDGDIWWEDL
EEECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCHHHHH
ENMPEHLTDWLGNDWTPESSQPSSHPNSRYCVPLAQCPVAAEEYNDPQGVPISAILFGGR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHCCCCCCCEEEEEEECCC
RPDTVPLVSQAHNWDHATMIGALLSSGQTAAAEGTVGALRHDPMAMLPFIGYNAADYLQH
CCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHCCCCHHHHHHH
WIDMGKKGGDKMPEVFLVNWFRRGDDGRFLWPGFGDNSRVLKWIVDRIEGRVEADETIAG
HHHHCCCCCCCCCCEEEEEHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
YTARVEDLDLTGLDTPIEDIREALSAPAAQWKSDLADNEEYLTFLGSKGSAVPAEVLEQF
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
EALKARVAAAES
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910