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The map label for this gene is shiA [H]

Identifier: 38234709

GI number: 38234709

Start: 2239756

End: 2241060

Strand: Direct

Name: shiA [H]

Synonym: DIP2166

Alternate gene names: 38234709

Gene position: 2239756-2241060 (Clockwise)

Preceding gene: 38234708

Following gene: 38234710

Centisome position: 90.0

GC content: 56.48

Gene sequence:

>1305_bases
ATGTTCATAAATATAAGTATGAAAAACTGCAACGAATCTTTGACCCCGCAGGGCCGTCGTGCGCTGTTGGCGGCCTTGTT
GGGAACCATCATTGAGTGGTACGACTACGCTCTCTATGGTGCCGCAGCTGCTGTCGTGATTGCCCCGCTCTTTTTCGAGT
CATCTGCGGCCGGCGGCCAGCTTGCCGCTTTTGCCACGTTTGCAGTTGGTTTTGTCGCACGCCCGTTAGGCGGTGTGGCC
ATTGGCCACATTGGTGATCGTTTTGGCCGTCGACCTGCGATGATCTTGAGTGTTTTGATGATGGGCATTGCCACGGTCGG
CATTGGTTTGCTCCCCACAGAGGCCGCTATTGGTGTGCTGGCTCCTGTGCTCTTGGTGTTCTTCCGTCTGATTCAAGGTT
TGGGTGCCGGTGCTGAACTTGCTGGTGCGATGACTGTGGTGGCCGAATTTGCCCCTGCGGCTAAGCGTGGACTTTTCACC
TCCTTGGTTTTGGCGATGCCTCCCGCGGGCATTGTGTTGGCGACTGCGGCGTTCATGGGGGCGTCGTCAGTAGGCGATGA
GGCATTGTTGGGTGGCGTGTGGCGTATCCCGTTCCTTATTTCTGCGCTGTTGTTCTTCATTGCGTTGTTCATGCGCAAGC
GTTTGGAGGAAACCCCAGAGTATGTAGCGGCCATGGAGGAGCGCTCTGAACAGGCAAAACACCAGTCTTTGCCGGTTGTG
GAGCTGCTCAAGTCGCATAAGCGTCAGGTGGCTTTGGGATTTTTGTCCATCACCGGTCACAACGCACTGAACTATGCGAT
GGCTGTTTTTGCCATCTCCTTTATGACTTCCGATGCGGTGGGCATGGCCAAGTTTGATGCTTTGCTGGCGGTTTCTGCCG
GAACCTTGGTGGGTGTGGTTGCTACCCCGCTGGGCGGGCTGGCCTCCGACCGATTCGGTGCTGATCGTGTATTGGCTACC
GGCTCATTAATCGGTGCTTTTTATGCAGTGCCCTTCCTTTTAGGTTTGCAAACGGGCGATGCGGTGATCGCCGCGCTGAC
GGTCGCTTTTGGCTATGGTGCGGTGATTTCGTTGACCTCGGGCAGCCAAGGCGCATTCCTTGCTGGGCTGTTTCAAGCAC
GCGCGCGTTTTAGTGGCATTGCGCTGGCTCGGGAAACCAACGGTGCTCTTATCGCTGGGTTCACCCCGCTGGTGTTGACG
TGGATCCTGCATGCCTCTGGCGGGAATGTACTTGCTGCCGGTGCTGTGGTGTCGGCATCCCTGCTGGTGTCTTTTGTTTC
GATCATGGTGGCCATGCGCCGCTAA

Upstream 100 bases:

>100_bases
CGCTGATGGGGTCAGCGGGGCGGGTGTGTGATGGCCTGTTTGACCAGATGTTGTAGTTCCCGAGTAAGCATAATGACATT
TTATGTAACGCCTTCTGCAT

Downstream 100 bases:

>100_bases
ATTCTTACAGCCATTCCACAGCTTGTACCCAGTGGAATTGAAACTGGTGTGCGATTGTGGGTGCGATCGGTGTGTGTTTG
CTGGCCTACCTTGCTCATGG

Product: ABC transporter permease

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MFINISMKNCNESLTPQGRRALLAALLGTIIEWYDYALYGAAAAVVIAPLFFESSAAGGQLAAFATFAVGFVARPLGGVA
IGHIGDRFGRRPAMILSVLMMGIATVGIGLLPTEAAIGVLAPVLLVFFRLIQGLGAGAELAGAMTVVAEFAPAAKRGLFT
SLVLAMPPAGIVLATAAFMGASSVGDEALLGGVWRIPFLISALLFFIALFMRKRLEETPEYVAAMEERSEQAKHQSLPVV
ELLKSHKRQVALGFLSITGHNALNYAMAVFAISFMTSDAVGMAKFDALLAVSAGTLVGVVATPLGGLASDRFGADRVLAT
GSLIGAFYAVPFLLGLQTGDAVIAALTVAFGYGAVISLTSGSQGAFLAGLFQARARFSGIALARETNGALIAGFTPLVLT
WILHASGGNVLAAGAVVSASLLVSFVSIMVAMRR

Sequences:

>Translated_434_residues
MFINISMKNCNESLTPQGRRALLAALLGTIIEWYDYALYGAAAAVVIAPLFFESSAAGGQLAAFATFAVGFVARPLGGVA
IGHIGDRFGRRPAMILSVLMMGIATVGIGLLPTEAAIGVLAPVLLVFFRLIQGLGAGAELAGAMTVVAEFAPAAKRGLFT
SLVLAMPPAGIVLATAAFMGASSVGDEALLGGVWRIPFLISALLFFIALFMRKRLEETPEYVAAMEERSEQAKHQSLPVV
ELLKSHKRQVALGFLSITGHNALNYAMAVFAISFMTSDAVGMAKFDALLAVSAGTLVGVVATPLGGLASDRFGADRVLAT
GSLIGAFYAVPFLLGLQTGDAVIAALTVAFGYGAVISLTSGSQGAFLAGLFQARARFSGIALARETNGALIAGFTPLVLT
WILHASGGNVLAAGAVVSASLLVSFVSIMVAMRR
>Mature_434_residues
MFINISMKNCNESLTPQGRRALLAALLGTIIEWYDYALYGAAAAVVIAPLFFESSAAGGQLAAFATFAVGFVARPLGGVA
IGHIGDRFGRRPAMILSVLMMGIATVGIGLLPTEAAIGVLAPVLLVFFRLIQGLGAGAELAGAMTVVAEFAPAAKRGLFT
SLVLAMPPAGIVLATAAFMGASSVGDEALLGGVWRIPFLISALLFFIALFMRKRLEETPEYVAAMEERSEQAKHQSLPVV
ELLKSHKRQVALGFLSITGHNALNYAMAVFAISFMTSDAVGMAKFDALLAVSAGTLVGVVATPLGGLASDRFGADRVLAT
GSLIGAFYAVPFLLGLQTGDAVIAALTVAFGYGAVISLTSGSQGAFLAGLFQARARFSGIALARETNGALIAGFTPLVLT
WILHASGGNVLAAGAVVSASLLVSFVSIMVAMRR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=35.3080568720379, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1789941, Length=406, Percent_Identity=34.2364532019704, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1790550, Length=403, Percent_Identity=31.0173697270471, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788942, Length=393, Percent_Identity=29.7709923664122, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082404, Length=321, Percent_Identity=23.6760124610592, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082231, Length=249, Percent_Identity=25.3012048192771, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44775; Mature: 44775

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFINISMKNCNESLTPQGRRALLAALLGTIIEWYDYALYGAAAAVVIAPLFFESSAAGGQ
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LAAFATFAVGFVARPLGGVAIGHIGDRFGRRPAMILSVLMMGIATVGIGLLPTEAAIGVL
HHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
APVLLVFFRLIQGLGAGAELAGAMTVVAEFAPAAKRGLFTSLVLAMPPAGIVLATAAFMG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
ASSVGDEALLGGVWRIPFLISALLFFIALFMRKRLEETPEYVAAMEERSEQAKHQSLPVV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHH
ELLKSHKRQVALGFLSITGHNALNYAMAVFAISFMTSDAVGMAKFDALLAVSAGTLVGVV
HHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ATPLGGLASDRFGADRVLATGSLIGAFYAVPFLLGLQTGDAVIAALTVAFGYGAVISLTS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GSQGAFLAGLFQARARFSGIALARETNGALIAGFTPLVLTWILHASGGNVLAAGAVVSAS
CCCCHHHHHHHHHHHHHHHEEEEEECCCCEEECHHHHHHHHHHHCCCCCEEEHHHHHHHH
LLVSFVSIMVAMRR
HHHHHHHHHHHHCC
>Mature Secondary Structure
MFINISMKNCNESLTPQGRRALLAALLGTIIEWYDYALYGAAAAVVIAPLFFESSAAGGQ
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LAAFATFAVGFVARPLGGVAIGHIGDRFGRRPAMILSVLMMGIATVGIGLLPTEAAIGVL
HHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
APVLLVFFRLIQGLGAGAELAGAMTVVAEFAPAAKRGLFTSLVLAMPPAGIVLATAAFMG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
ASSVGDEALLGGVWRIPFLISALLFFIALFMRKRLEETPEYVAAMEERSEQAKHQSLPVV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHH
ELLKSHKRQVALGFLSITGHNALNYAMAVFAISFMTSDAVGMAKFDALLAVSAGTLVGVV
HHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ATPLGGLASDRFGADRVLATGSLIGAFYAVPFLLGLQTGDAVIAALTVAFGYGAVISLTS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GSQGAFLAGLFQARARFSGIALARETNGALIAGFTPLVLTWILHASGGNVLAAGAVVSAS
CCCCHHHHHHHHHHHHHHHEEEEEECCCCEEECHHHHHHHHHHHCCCCCEEEHHHHHHHH
LLVSFVSIMVAMRR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]