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The map label for this gene is nikB [C]

Identifier: 38234706

GI number: 38234706

Start: 2236473

End: 2237426

Strand: Direct

Name: nikB [C]

Synonym: DIP2163

Alternate gene names: 38234706

Gene position: 2236473-2237426 (Clockwise)

Preceding gene: 38234705

Following gene: 38234707

Centisome position: 89.87

GC content: 61.22

Gene sequence:

>954_bases
GTGCCACATCTGTCCCACCGCGCTTCGTCCATCATTCGCCTCGCACTGCGCGCACCGGTCTTGCTGGTAGCCGCAGCGAT
GACGAGTTTTGTCATCATGGATGTCCTTCCAGGTGATGCCGCCAGCACCATTGCGCGCACTACGGACCCCGCCACTGTCG
ACGCGGTCCGTAGTCGCCTTGGACTCGACGATCCCCTCTTAGTGCGGTTGGGCGAGTGGGCCACTGCGCTATTTCTTCAC
GGCGACGGCGGCCACCTCTACGCCTCGGGGATACCCGTGTGGGAGGCTGCGGCGAGCTCGGCACGAAATTCCACGTACCT
TGTTGCCTGCGCACTCCCACTGCTGCTCCTTATCGGAATTCTGAGCGGTGTTTTCGCAGGTCTACGCCCTACGGCATTGC
GTGATCACGTACTTTCTACCGGTGCACAAACCACACTGGCTACTCCTGATTTCGCTGTCACCACCGTGTTGTTGGTCCTC
TGTGCGGGAGTGCTGCATCTGGCACCGGCAGTCTCTCTGGTTCCCCCAGGTGGAACACCACTAGATCGCCCCGATTCTCT
AATTGTTCCAGCGCTCGCCATCGCGCTTGTCGGTGGCGCATGGCTGCAACGTCTCGTGCGTGCAGCGATTGTCGACGCCG
CCGCGCTCCCCCATATTCGCGCCGCACACCTCGCCGGTCACCATCCTGTAACAGTGGCCTGGCGTCTCACACTTCCTGCC
GCAGCAGGCCCCATTGTCCAAGCATGTGCCGCCACCATCCCCTACGCTGTCACCGGCACCGTTGTTGTAGAAAACGTGGT
GGGATTCCCAGGAATCGGCACGCTCATCGCCACATTCATCGCAGCACGTGAAACCATCGCCGTGGCAACGCTCACCGCAG
TGTTTGCTGCCATTACCATCGCAGCGTTCACCTTGGCTGATCTCCACCACGACATCCAAGGACGCCGCGCATGA

Upstream 100 bases:

>100_bases
GGGCTACGCAAATGAGGTCCACGGGCGTGCCGACGGCATCCCTGACGTGCCGGTTTCTCAAAGCGTCCCTGTTCCTACCA
CCAAGTAATCCCACGGTATC

Downstream 100 bases:

>100_bases
TTCACCTTCGCCGCCCATCCCTGCACACCGTCACCACCTCTGCGTTATGGCTGCTGTGGATTCTTACACTTATCGCAGCC
ATCGCCGGCCCCACCGTGGC

Product: ABC transporter permease

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPHLSHRASSIIRLALRAPVLLVAAAMTSFVIMDVLPGDAASTIARTTDPATVDAVRSRLGLDDPLLVRLGEWATALFLH
GDGGHLYASGIPVWEAAASSARNSTYLVACALPLLLLIGILSGVFAGLRPTALRDHVLSTGAQTTLATPDFAVTTVLLVL
CAGVLHLAPAVSLVPPGGTPLDRPDSLIVPALAIALVGGAWLQRLVRAAIVDAAALPHIRAAHLAGHHPVTVAWRLTLPA
AAGPIVQACAATIPYAVTGTVVVENVVGFPGIGTLIATFIAARETIAVATLTAVFAAITIAAFTLADLHHDIQGRRA

Sequences:

>Translated_317_residues
MPHLSHRASSIIRLALRAPVLLVAAAMTSFVIMDVLPGDAASTIARTTDPATVDAVRSRLGLDDPLLVRLGEWATALFLH
GDGGHLYASGIPVWEAAASSARNSTYLVACALPLLLLIGILSGVFAGLRPTALRDHVLSTGAQTTLATPDFAVTTVLLVL
CAGVLHLAPAVSLVPPGGTPLDRPDSLIVPALAIALVGGAWLQRLVRAAIVDAAALPHIRAAHLAGHHPVTVAWRLTLPA
AAGPIVQACAATIPYAVTGTVVVENVVGFPGIGTLIATFIAARETIAVATLTAVFAAITIAAFTLADLHHDIQGRRA
>Mature_316_residues
PHLSHRASSIIRLALRAPVLLVAAAMTSFVIMDVLPGDAASTIARTTDPATVDAVRSRLGLDDPLLVRLGEWATALFLHG
DGGHLYASGIPVWEAAASSARNSTYLVACALPLLLLIGILSGVFAGLRPTALRDHVLSTGAQTTLATPDFAVTTVLLVLC
AGVLHLAPAVSLVPPGGTPLDRPDSLIVPALAIALVGGAWLQRLVRAAIVDAAALPHIRAAHLAGHHPVTVAWRLTLPAA
AGPIVQACAATIPYAVTGTVVVENVVGFPGIGTLIATFIAARETIAVATLTAVFAAITIAAFTLADLHHDIQGRRA

Specific function: Probably part of an ABC transporter complex that could be involved in peptide import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=306, Percent_Identity=27.4509803921569, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787497, Length=301, Percent_Identity=27.2425249169435, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787761, Length=309, Percent_Identity=30.0970873786408, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787053, Length=275, Percent_Identity=26.1818181818182, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789965, Length=329, Percent_Identity=22.7963525835866, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32552; Mature: 32420

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLSHRASSIIRLALRAPVLLVAAAMTSFVIMDVLPGDAASTIARTTDPATVDAVRSRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHH
GLDDPLLVRLGEWATALFLHGDGGHLYASGIPVWEAAASSARNSTYLVACALPLLLLIGI
CCCCHHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSGVFAGLRPTALRDHVLSTGAQTTLATPDFAVTTVLLVLCAGVLHLAPAVSLVPPGGTP
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
LDRPDSLIVPALAIALVGGAWLQRLVRAAIVDAAALPHIRAAHLAGHHPVTVAWRLTLPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCC
AAGPIVQACAATIPYAVTGTVVVENVVGFPGIGTLIATFIAARETIAVATLTAVFAAITI
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFTLADLHHDIQGRRA
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PHLSHRASSIIRLALRAPVLLVAAAMTSFVIMDVLPGDAASTIARTTDPATVDAVRSRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHH
GLDDPLLVRLGEWATALFLHGDGGHLYASGIPVWEAAASSARNSTYLVACALPLLLLIGI
CCCCHHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSGVFAGLRPTALRDHVLSTGAQTTLATPDFAVTTVLLVLCAGVLHLAPAVSLVPPGGTP
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
LDRPDSLIVPALAIALVGGAWLQRLVRAAIVDAAALPHIRAAHLAGHHPVTVAWRLTLPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCC
AAGPIVQACAATIPYAVTGTVVVENVVGFPGIGTLIATFIAARETIAVATLTAVFAAITI
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFTLADLHHDIQGRRA
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]