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The map label for this gene is pitB [C]

Identifier: 38234678

GI number: 38234678

Start: 2182285

End: 2183871

Strand: Reverse

Name: pitB [C]

Synonym: DIP2129

Alternate gene names: 38234678

Gene position: 2183871-2182285 (Counterclockwise)

Preceding gene: 38234680

Following gene: 38234677

Centisome position: 87.75

GC content: 58.73

Gene sequence:

>1587_bases
ATGCTCAGTGGCGCTCCCTTCGATCAGCGCTCCGACAAATGGTGGCACCTCACCTTTGGCGGAATGCTCGCAGTGGCGCT
GATCTTCTTCATCACATGGTCCATGGGATACGTTGGCCCCGAAGCCAACAAAGCCATGCTGATCACCACGATCGCCTTCG
GCGTATTCATGGCCTTCAACATCGGCGGCAACGATGTGGCCAACTCCTTTGGCACCTCGGTGGGTGCGGGAACACTCAGC
ATGAAACAAGCGCTGGTGATCGCCGCAATCTTCGAAGTATCCGGCGCTGTCCTCGCAGGCGGCGAGGTCACCGACACAGT
TAAATCCGGCATCGTTGACCTCGGTGCCATCGACCTCGACCCCCAACACTTCGCCTTCATCATGATGGCCTCGCTCCTCG
GAGCCGCAGTATGGCTCCTCGTGGCCACCAAGATGGGCTGGCCAGTGTCCACCACGCACTCCATCGTCGGCGGTATCGTC
GGTGCCTCGCTGCTCTTGGGCTTTACCCAAGGAATCGGCGGCTGGGAAATGGTGCAATGGGCAGAAATCGGCCGCATTGC
CATGTCCTGGGTGCTTTCGCCACTACTTGGCGGCCTCTGTGCGTGGTTGCTCTTCGGCTTTATTAAAAAGCACATTTTGG
TCTACAACGAAGAAGCCGACGAGGCGCTTCGTCAGATCAAAGCCGACCGCATTGAGCTGCACCAATCCTTCAAAGCCTCC
TTCGAGCGCCTCAACGAGATCCAGCAGTTGGCCTACACCAACGCCATGACTCGCGACGCCGCCTTGATCCAAGAACCAGA
CTTCGACCCAGAACAACTGGAGTCTGACTACTACCGCAACCTGTACCGCATCAATACCCGACGCGACAACCTAAACACCC
ACCGTGCGTTGGAAAACTGGGTGCCGCTGCTGGCTGCTGCAGGCGCCGCCCTTATCGGTGCCATGATGCTGTTCAAAGGG
CTTAAGAACCTCAACTTGCATATCAGCAACTTGGGCAACGTGGTTATCCTCGTGATGCTCTCCGCCGTAGTCTGGATGGC
CATTTCGATCTTCTCCCGACTGCTGCGCCAGCAAGAACTCACACGGGCAACCTTTGTGCTGTTCTCTTGGATGCAGGTAT
TTACCGCCTCCGCCTTCGCGTTCTCCCACGGTTCGAACGACATCGCCAACGCAATCGGACCATTTTCCGCAGTGCTCGAC
GTCCTCCGCACCGACTCCATCAACGGCAAAGCCGCAGTGCCAACCGCACTGATGGTCACCTGCGGTATCTCACTGATCGC
CGGCCTGTGGTTCATCGGCCGCTACGTGATCCACACCGTGGGCACCGGATTAACCAAGATGCACCCTGCCTCCGGCTTCG
CAGCTGAACTTTCCGCCGCAGCGGTAGTCATGGGCGCATCCGTCATCGGCTTGCCGGTGTCTTCCACTCACATCTTGATC
GGCGCGATCCTCGGTATCGGCGTGGTCAACAAATCCGCCAACTGGCGGCTCATGAAGCCCATCGGCATGGCGTGGATTAT
CACCCTGCCGGCTGCAGCCGCAGTATCCGGCGCAGCAGTGCTCGTGCTCAACGCCGTGTGGGGGTAA

Upstream 100 bases:

>100_bases
GCGATCTCCGTGTAACTTTCTGTTCACCTTGATGAGGGAATATGACCAACAGGTTTTTGCTTGAATCAACAGAAAGTTGA
GCACTATGACGGACAGCGCG

Downstream 100 bases:

>100_bases
TACCTGCGTGTACGGCGAAAAATCCACACCACATGCTCGAAAACTCGCGGTTTCTCGAGGATGCGGTGTGGATTTTGCTA
TCCGCGGGGGACGCGGGTGT

Product: putative binding-protein-dependent integral membrane transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MLSGAPFDQRSDKWWHLTFGGMLAVALIFFITWSMGYVGPEANKAMLITTIAFGVFMAFNIGGNDVANSFGTSVGAGTLS
MKQALVIAAIFEVSGAVLAGGEVTDTVKSGIVDLGAIDLDPQHFAFIMMASLLGAAVWLLVATKMGWPVSTTHSIVGGIV
GASLLLGFTQGIGGWEMVQWAEIGRIAMSWVLSPLLGGLCAWLLFGFIKKHILVYNEEADEALRQIKADRIELHQSFKAS
FERLNEIQQLAYTNAMTRDAALIQEPDFDPEQLESDYYRNLYRINTRRDNLNTHRALENWVPLLAAAGAALIGAMMLFKG
LKNLNLHISNLGNVVILVMLSAVVWMAISIFSRLLRQQELTRATFVLFSWMQVFTASAFAFSHGSNDIANAIGPFSAVLD
VLRTDSINGKAAVPTALMVTCGISLIAGLWFIGRYVIHTVGTGLTKMHPASGFAAELSAAAVVMGASVIGLPVSSTHILI
GAILGIGVVNKSANWRLMKPIGMAWIITLPAAAAVSGAAVLVLNAVWG

Sequences:

>Translated_528_residues
MLSGAPFDQRSDKWWHLTFGGMLAVALIFFITWSMGYVGPEANKAMLITTIAFGVFMAFNIGGNDVANSFGTSVGAGTLS
MKQALVIAAIFEVSGAVLAGGEVTDTVKSGIVDLGAIDLDPQHFAFIMMASLLGAAVWLLVATKMGWPVSTTHSIVGGIV
GASLLLGFTQGIGGWEMVQWAEIGRIAMSWVLSPLLGGLCAWLLFGFIKKHILVYNEEADEALRQIKADRIELHQSFKAS
FERLNEIQQLAYTNAMTRDAALIQEPDFDPEQLESDYYRNLYRINTRRDNLNTHRALENWVPLLAAAGAALIGAMMLFKG
LKNLNLHISNLGNVVILVMLSAVVWMAISIFSRLLRQQELTRATFVLFSWMQVFTASAFAFSHGSNDIANAIGPFSAVLD
VLRTDSINGKAAVPTALMVTCGISLIAGLWFIGRYVIHTVGTGLTKMHPASGFAAELSAAAVVMGASVIGLPVSSTHILI
GAILGIGVVNKSANWRLMKPIGMAWIITLPAAAAVSGAAVLVLNAVWG
>Mature_528_residues
MLSGAPFDQRSDKWWHLTFGGMLAVALIFFITWSMGYVGPEANKAMLITTIAFGVFMAFNIGGNDVANSFGTSVGAGTLS
MKQALVIAAIFEVSGAVLAGGEVTDTVKSGIVDLGAIDLDPQHFAFIMMASLLGAAVWLLVATKMGWPVSTTHSIVGGIV
GASLLLGFTQGIGGWEMVQWAEIGRIAMSWVLSPLLGGLCAWLLFGFIKKHILVYNEEADEALRQIKADRIELHQSFKAS
FERLNEIQQLAYTNAMTRDAALIQEPDFDPEQLESDYYRNLYRINTRRDNLNTHRALENWVPLLAAAGAALIGAMMLFKG
LKNLNLHISNLGNVVILVMLSAVVWMAISIFSRLLRQQELTRATFVLFSWMQVFTASAFAFSHGSNDIANAIGPFSAVLD
VLRTDSINGKAAVPTALMVTCGISLIAGLWFIGRYVIHTVGTGLTKMHPASGFAAELSAAAVVMGASVIGLPVSSTHILI
GAILGIGVVNKSANWRLMKPIGMAWIITLPAAAAVSGAAVLVLNAVWG

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=163, Percent_Identity=40.4907975460123, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI5803173, Length=181, Percent_Identity=38.6740331491713, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71981576, Length=509, Percent_Identity=30.4518664047151, Blast_Score=222, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25146401, Length=171, Percent_Identity=39.1812865497076, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566716, Length=167, Percent_Identity=40.7185628742515, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17539280, Length=195, Percent_Identity=36.9230769230769, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17536725, Length=168, Percent_Identity=33.9285714285714, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17557328, Length=222, Percent_Identity=27.9279279279279, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319773, Length=154, Percent_Identity=36.3636363636364, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356511, Length=176, Percent_Identity=44.8863636363636, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56561; Mature: 56561

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGAPFDQRSDKWWHLTFGGMLAVALIFFITWSMGYVGPEANKAMLITTIAFGVFMAFN
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCC
IGGNDVANSFGTSVGAGTLSMKQALVIAAIFEVSGAVLAGGEVTDTVKSGIVDLGAIDLD
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCCCC
PQHFAFIMMASLLGAAVWLLVATKMGWPVSTTHSIVGGIVGASLLLGFTQGIGGWEMVQW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AEIGRIAMSWVLSPLLGGLCAWLLFGFIKKHILVYNEEADEALRQIKADRIELHQSFKAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHH
FERLNEIQQLAYTNAMTRDAALIQEPDFDPEQLESDYYRNLYRINTRRDNLNTHRALENW
HHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHH
VPLLAAAGAALIGAMMLFKGLKNLNLHISNLGNVVILVMLSAVVWMAISIFSRLLRQQEL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRATFVLFSWMQVFTASAFAFSHGSNDIANAIGPFSAVLDVLRTDSINGKAAVPTALMVT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CGISLIAGLWFIGRYVIHTVGTGLTKMHPASGFAAELSAAAVVMGASVIGLPVSSTHILI
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCHHEECCCCCHHHHH
GAILGIGVVNKSANWRLMKPIGMAWIITLPAAAAVSGAAVLVLNAVWG
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHEEEEECCC
>Mature Secondary Structure
MLSGAPFDQRSDKWWHLTFGGMLAVALIFFITWSMGYVGPEANKAMLITTIAFGVFMAFN
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCC
IGGNDVANSFGTSVGAGTLSMKQALVIAAIFEVSGAVLAGGEVTDTVKSGIVDLGAIDLD
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCCCC
PQHFAFIMMASLLGAAVWLLVATKMGWPVSTTHSIVGGIVGASLLLGFTQGIGGWEMVQW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AEIGRIAMSWVLSPLLGGLCAWLLFGFIKKHILVYNEEADEALRQIKADRIELHQSFKAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHH
FERLNEIQQLAYTNAMTRDAALIQEPDFDPEQLESDYYRNLYRINTRRDNLNTHRALENW
HHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHH
VPLLAAAGAALIGAMMLFKGLKNLNLHISNLGNVVILVMLSAVVWMAISIFSRLLRQQEL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRATFVLFSWMQVFTASAFAFSHGSNDIANAIGPFSAVLDVLRTDSINGKAAVPTALMVT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CGISLIAGLWFIGRYVIHTVGTGLTKMHPASGFAAELSAAAVVMGASVIGLPVSSTHILI
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCHHEECCCCCHHHHH
GAILGIGVVNKSANWRLMKPIGMAWIITLPAAAAVSGAAVLVLNAVWG
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]