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The map label for this gene is ackA

Identifier: 38234611

GI number: 38234611

Start: 2102477

End: 2103676

Strand: Reverse

Name: ackA

Synonym: DIP2054

Alternate gene names: 38234611

Gene position: 2103676-2102477 (Counterclockwise)

Preceding gene: 38234612

Following gene: 38234607

Centisome position: 84.53

GC content: 55.92

Gene sequence:

>1200_bases
ATGGCGCTTGTCCTCGTCCTCAATTCTGGTTCTTCATCCATCAAGTTCCAGCTGGTAGATCCTACCGCCCACGCCACTGA
CGACCCGTTTGCCTCTGGTTTGGTGGAACAGATTGGTGAGCCACAGGGTCGAGTGACTCTGAAGCACGCTGGTGAGAAGT
TCGTCGTCGAGGCACCGATTCCTGATCACTCCGCTGGTTTGGCGTTGGCTTTCGATCTGATGGGTGAGCACAAGTGTGGT
CCTACTGATGTGGAGATCATTGCTGTGGGTCACCGCGTGGTTCACGGTGGTATTTTGTTCTCCCAGCCGCAGGTAATTAC
GGATGAGATCATGGACATGGTCCGCGATCTGATTCCGCTGGCTCCCCTGCACAACCCTGCCAACATTGATGGCATTGAGG
TTGCTCGCAAGATCTTGCCTGATGTGGCACACGTTGCGGTCTTTGATACTGGTTTCTTCCACGATATGCCACCAGCGGCA
GCGATTTACGCTATCGACGCCAAGACTGCCGCGGATCATGGTGTGCGTCGCTATGGCTTCCACGGCACCAGCCACGAGTA
TGTGTCTCATAAGGTGGCTGAGCTGCTGGATCTTCCGGAAGGCGCTATCAACCAGATCACCTTGCACCTAGGTAATGGTG
CTTCGTGCGCTGCTATCCGTGGTGGCAAGGCTATTGACACCTCGATGGGCATGACCCCCTTGTCTGGTCTTGTGATGGGT
ACGCGTTCGGGTGATATTGACCCAGGTATTGTGTTCCACTTGCACCGTCAGGCGGGTATGAGCATTGATGAGATCGATGA
GCTGCTGAACAAGAAGTCCGGTGTGAAGGGCATCTCGGGGGTTAATGACTTCCGTGAGCTGCGCACCATGATTGACGCCG
GCGACCAGGACGCTTGGCTGGCGTACAACATTTACATTCACCAGCTGCGTCGCTACATCGGTTCCTACATGATCGCCCTT
GGTCGCGTAAACGCTATTACGTTCACCGCAGGTGTTGGCGAGAACGACGTGGCCGTGCGTGCCGACGCCCTCTCGCACCT
CGAAGGCTTTGGCATCAAGATCGACCCAGAGCGCAACGCATTGCCAAACACTGGCCCACGTGAAATCTCGACGGACGATT
CCGCCATCAAGGTTTTCGTGGTTCCTACCAACGAGGAGCTCGCTATCGCACGCTACGCCAAGGCACTCTCCGGCGCATAA

Upstream 100 bases:

>100_bases
GCAGGGCTTGAACAAGCCTGTGAACGACCTTTCTCGCGGCGCAACCGTTGCCGACATTGTGAACACCGTAGCCATTACTG
CAATTCAAGGAGGCAAATAA

Downstream 100 bases:

>100_bases
GCCGGTAGGGCGTTATGCCTTAGAACCAGGTCATGGGGCGGACCTGGTTGGCTACGTCGACAAGCGCGTAGCGATGTGAG
CTGGGGGCGCTGCGGGCGAG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MALVLVLNSGSSSIKFQLVDPTAHATDDPFASGLVEQIGEPQGRVTLKHAGEKFVVEAPIPDHSAGLALAFDLMGEHKCG
PTDVEIIAVGHRVVHGGILFSQPQVITDEIMDMVRDLIPLAPLHNPANIDGIEVARKILPDVAHVAVFDTGFFHDMPPAA
AIYAIDAKTAADHGVRRYGFHGTSHEYVSHKVAELLDLPEGAINQITLHLGNGASCAAIRGGKAIDTSMGMTPLSGLVMG
TRSGDIDPGIVFHLHRQAGMSIDEIDELLNKKSGVKGISGVNDFRELRTMIDAGDQDAWLAYNIYIHQLRRYIGSYMIAL
GRVNAITFTAGVGENDVAVRADALSHLEGFGIKIDPERNALPNTGPREISTDDSAIKVFVVPTNEELAIARYAKALSGA

Sequences:

>Translated_399_residues
MALVLVLNSGSSSIKFQLVDPTAHATDDPFASGLVEQIGEPQGRVTLKHAGEKFVVEAPIPDHSAGLALAFDLMGEHKCG
PTDVEIIAVGHRVVHGGILFSQPQVITDEIMDMVRDLIPLAPLHNPANIDGIEVARKILPDVAHVAVFDTGFFHDMPPAA
AIYAIDAKTAADHGVRRYGFHGTSHEYVSHKVAELLDLPEGAINQITLHLGNGASCAAIRGGKAIDTSMGMTPLSGLVMG
TRSGDIDPGIVFHLHRQAGMSIDEIDELLNKKSGVKGISGVNDFRELRTMIDAGDQDAWLAYNIYIHQLRRYIGSYMIAL
GRVNAITFTAGVGENDVAVRADALSHLEGFGIKIDPERNALPNTGPREISTDDSAIKVFVVPTNEELAIARYAKALSGA
>Mature_398_residues
ALVLVLNSGSSSIKFQLVDPTAHATDDPFASGLVEQIGEPQGRVTLKHAGEKFVVEAPIPDHSAGLALAFDLMGEHKCGP
TDVEIIAVGHRVVHGGILFSQPQVITDEIMDMVRDLIPLAPLHNPANIDGIEVARKILPDVAHVAVFDTGFFHDMPPAAA
IYAIDAKTAADHGVRRYGFHGTSHEYVSHKVAELLDLPEGAINQITLHLGNGASCAAIRGGKAIDTSMGMTPLSGLVMGT
RSGDIDPGIVFHLHRQAGMSIDEIDELLNKKSGVKGISGVNDFRELRTMIDAGDQDAWLAYNIYIHQLRRYIGSYMIALG
RVNAITFTAGVGENDVAVRADALSHLEGFGIKIDPERNALPNTGPREISTDDSAIKVFVVPTNEELAIARYAKALSGA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=403, Percent_Identity=43.1761786600496, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI145693184, Length=394, Percent_Identity=42.6395939086294, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_CORDI (Q6NF47)

Other databases:

- EMBL:   BX248360
- RefSeq:   NP_940378.1
- ProteinModelPortal:   Q6NF47
- SMR:   Q6NF47
- GeneID:   2650153
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2054
- NMPDR:   fig|257309.1.peg.1969
- HOGENOM:   HBG285863
- OMA:   LNRHSGL
- PhylomeDB:   Q6NF47
- ProtClustDB:   PRK00180
- BioCyc:   CDIP257309:DIP2054-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42635; Mature: 42504

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLVLNSGSSSIKFQLVDPTAHATDDPFASGLVEQIGEPQGRVTLKHAGEKFVVEAPI
CEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
PDHSAGLALAFDLMGEHKCGPTDVEIIAVGHRVVHGGILFSQPQVITDEIMDMVRDLIPL
CCCCCCCEEEEEHHCCCCCCCCCEEEEEECCEEEECCEEECCCCHHHHHHHHHHHHHHCC
APLHNPANIDGIEVARKILPDVAHVAVFDTGFFHDMPPAAAIYAIDAKTAADHGVRRYGF
CCCCCCCCCCHHHHHHHHCCHHHHEEEEECCCCCCCCCCCEEEEEECCHHHHCCHHHHCC
HGTSHEYVSHKVAELLDLPEGAINQITLHLGNGASCAAIRGGKAIDTSMGMTPLSGLVMG
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCEECCCCCCCCCCCEEEE
TRSGDIDPGIVFHLHRQAGMSIDEIDELLNKKSGVKGISGVNDFRELRTMIDAGDQDAWL
CCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEE
AYNIYIHQLRRYIGSYMIALGRVNAITFTAGVGENDVAVRADALSHLEGFGIKIDPERNA
EEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHHHHHHCCCCEEECCCCCC
LPNTGPREISTDDSAIKVFVVPTNEELAIARYAKALSGA
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ALVLVLNSGSSSIKFQLVDPTAHATDDPFASGLVEQIGEPQGRVTLKHAGEKFVVEAPI
EEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
PDHSAGLALAFDLMGEHKCGPTDVEIIAVGHRVVHGGILFSQPQVITDEIMDMVRDLIPL
CCCCCCCEEEEEHHCCCCCCCCCEEEEEECCEEEECCEEECCCCHHHHHHHHHHHHHHCC
APLHNPANIDGIEVARKILPDVAHVAVFDTGFFHDMPPAAAIYAIDAKTAADHGVRRYGF
CCCCCCCCCCHHHHHHHHCCHHHHEEEEECCCCCCCCCCCEEEEEECCHHHHCCHHHHCC
HGTSHEYVSHKVAELLDLPEGAINQITLHLGNGASCAAIRGGKAIDTSMGMTPLSGLVMG
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCEECCCCCCCCCCCEEEE
TRSGDIDPGIVFHLHRQAGMSIDEIDELLNKKSGVKGISGVNDFRELRTMIDAGDQDAWL
CCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEE
AYNIYIHQLRRYIGSYMIALGRVNAITFTAGVGENDVAVRADALSHLEGFGIKIDPERNA
EEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHHHHHHCCCCEEECCCCCC
LPNTGPREISTDDSAIKVFVVPTNEELAIARYAKALSGA
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910