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The map label for this gene is xthA [H]

Identifier: 38234604

GI number: 38234604

Start: 2093716

End: 2094423

Strand: Direct

Name: xthA [H]

Synonym: DIP2047

Alternate gene names: 38234604

Gene position: 2093716-2094423 (Clockwise)

Preceding gene: 38234603

Following gene: 38234605

Centisome position: 84.13

GC content: 59.32

Gene sequence:

>708_bases
ATGCGCATTGCCAACTGGAACGTGAACTCCGCCCGGGCGCGAGTAGACCGAATACAGGACTTCCTCGAACGCCACGACAT
TGACGTCCTTGCCATGCAAGAAACCAAATGCCGCGACGACCAGTTCCCCGAATTCAAAGGCTACGAAGCCGCCCACTTTG
GCATCAACCAATGGAACGGCGTAGCTATTCTTTCTCGGGTTGGCATCGAGGACGTCCTTACCAGCTTCGGGCAACCCGAC
TACGAAGAATTCGAACCACGTTCCATCGGCGCAATCTGCGGCGGCGTGCGCGTGTGGAGCCTCTACGTGCCCAACGGCCG
CGAAATCGCCCACCCCCACTACGACTACAAACTGCGCTGGCTCTACGCCCTCCAACACTACGGCGTCACCGGCGACACCG
TCTGGTGCGGCGACTTCAACATCGCCCCCACCGACAACGACGTCTGGGACATCGACTTCTTCCACGGCAAAACCCACGTC
ACCGAACCAGAACGCCAAGCCTTCGAAGCACTGACCGACATCATGCCGATCACCTCCCCCGAGGGCTACACCTACTGGGA
CTACACCGCTGGCCGATTCCCCAAAAACCAAGGAATGCGCATCGACTTCCAGCTCTCCAACATGACCCCCACCGGCGGAT
TCATCGACGTTGAAGAGCGCAAAGGCAAGGGGGCTTCCGATCACGCCCCAGTGGTGGTGGACTATTGA

Upstream 100 bases:

>100_bases
CTGCAGGTCATTGAGAGCAATGCCGCCGGAATCGGGCTGTACCACAAATTAGGGTTTGTGGAACATCATCGTCACAAGTA
CGTGCGGGTATCCTAGCAAC

Downstream 100 bases:

>100_bases
TAGATTGGCAAACAGCTGGCCTGATCATCGACTACGCCATCAAAATCGTAGCCATCGGATTCGTCCCCGAAGGCCGTCGT
CCCTCCAGCTCCACCGCATG

Product: putative exonuclease

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRIANWNVNSARARVDRIQDFLERHDIDVLAMQETKCRDDQFPEFKGYEAAHFGINQWNGVAILSRVGIEDVLTSFGQPD
YEEFEPRSIGAICGGVRVWSLYVPNGREIAHPHYDYKLRWLYALQHYGVTGDTVWCGDFNIAPTDNDVWDIDFFHGKTHV
TEPERQAFEALTDIMPITSPEGYTYWDYTAGRFPKNQGMRIDFQLSNMTPTGGFIDVEERKGKGASDHAPVVVDY

Sequences:

>Translated_235_residues
MRIANWNVNSARARVDRIQDFLERHDIDVLAMQETKCRDDQFPEFKGYEAAHFGINQWNGVAILSRVGIEDVLTSFGQPD
YEEFEPRSIGAICGGVRVWSLYVPNGREIAHPHYDYKLRWLYALQHYGVTGDTVWCGDFNIAPTDNDVWDIDFFHGKTHV
TEPERQAFEALTDIMPITSPEGYTYWDYTAGRFPKNQGMRIDFQLSNMTPTGGFIDVEERKGKGASDHAPVVVDY
>Mature_235_residues
MRIANWNVNSARARVDRIQDFLERHDIDVLAMQETKCRDDQFPEFKGYEAAHFGINQWNGVAILSRVGIEDVLTSFGQPD
YEEFEPRSIGAICGGVRVWSLYVPNGREIAHPHYDYKLRWLYALQHYGVTGDTVWCGDFNIAPTDNDVWDIDFFHGKTHV
TEPERQAFEALTDIMPITSPEGYTYWDYTAGRFPKNQGMRIDFQLSNMTPTGGFIDVEERKGKGASDHAPVVVDY

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=30.9701492537313, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=26.8774703557312, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 26949; Mature: 26949

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIANWNVNSARARVDRIQDFLERHDIDVLAMQETKCRDDQFPEFKGYEAAHFGINQWNG
CEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCC
VAILSRVGIEDVLTSFGQPDYEEFEPRSIGAICGGVRVWSLYVPNGREIAHPHYDYKLRW
EEEEECCCHHHHHHHCCCCCHHHCCCCHHHHHHCCEEEEEEECCCCCEECCCCCCEEEEE
LYALQHYGVTGDTVWCGDFNIAPTDNDVWDIDFFHGKTHVTEPERQAFEALTDIMPITSP
EEEEHHCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
EGYTYWDYTAGRFPKNQGMRIDFQLSNMTPTGGFIDVEERKGKGASDHAPVVVDY
CCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRIANWNVNSARARVDRIQDFLERHDIDVLAMQETKCRDDQFPEFKGYEAAHFGINQWNG
CEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCC
VAILSRVGIEDVLTSFGQPDYEEFEPRSIGAICGGVRVWSLYVPNGREIAHPHYDYKLRW
EEEEECCCHHHHHHHCCCCCHHHCCCCHHHHHHCCEEEEEEECCCCCEECCCCCCEEEEE
LYALQHYGVTGDTVWCGDFNIAPTDNDVWDIDFFHGKTHVTEPERQAFEALTDIMPITSP
EEEEHHCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
EGYTYWDYTAGRFPKNQGMRIDFQLSNMTPTGGFIDVEERKGKGASDHAPVVVDY
CCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]