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The map label for this gene is nemA [H]

Identifier: 38234590

GI number: 38234590

Start: 2080479

End: 2081561

Strand: Reverse

Name: nemA [H]

Synonym: DIP2030

Alternate gene names: 38234590

Gene position: 2081561-2080479 (Counterclockwise)

Preceding gene: 38234593

Following gene: 38234589

Centisome position: 83.64

GC content: 48.85

Gene sequence:

>1083_bases
ATGACGACCCTTTTTGAATCCGCCACCGCAGGCGTCCTTGAATTGAACAATCGCATCACTATGGCCCCCTTGACCCGTTC
CCGTGCTGGGCAAGACGGCGTTCCTACCGATATGCACGTGGAATACTACAAGCAACGCGCGAATAATGGTCTGATTGTCA
CTGAAGGTACATTCTTTGAAGACATCAACCGTGCATTTTTAGGTCAGCCTGGGATCGCCACCGCCGATCAACAAGAAGGA
TGGCGTAAGGTTGCCCAGGCTGTTCACGAACAGGGTAGCAACATTGTGATGCAAATTATGCATGGAGGACGCCTTACTCT
ACCTTCTATTAATGGCTTCGACCAAGGAGAGTCATCGAGCGCTATTGCTCCCGGGGTTACGTTGCACGGTGATGGAGAGT
CCCGAGAAGAGGTGCCCACACCTGTTGCTCTGGATGCCGAGGGTATTAAAAGGGTTATTCAGAAATTTACCGAAGGCGCC
CGACGTGCCATTGATGCAGGTATGGATGGGGTAGAAGTGCACGGTGCTAACGGTTATCTTCTTCACCAATTCCTCGCCCC
TAGTTCTAACGACCGGACAGATGAGTATGGTGGTAGCCCTGAGAATCGTGCTCGTTTAACCATTGAGGTGATTCGCTCTA
TCGCAGATGAAATCGGAGCAGACCGGGTGGGCTTACGTATTTCTCCTATGCATAATATTCAAGGTTGCATTGAGGAGGAT
GAGGATGATGTGCGCGCTACGTATAAAGCGTTGTTGGATGGTATTGCTGACCTGAATATCGCTTATCTCTCTATTTTGTA
TCAGGATGCAGAAAGCGACTTGGTTGCTTATTTGCGAGAATCCTTTGGTGGTTTCACCATTCTCAATACCGGTTTTGGTG
CGGTGACAGAGTTAGAAGACGCTCGTTATATTGTAGAGAATGGCTTGGCTGATGCGGTGGCAGTGGGGCGTGAGATAATT
GCTAATCCCGATTTGTCGTATCGGTGGAAGAATAATTTAGCGTTGAATTCAATTGATTTCGATACTTTTTACACGCCCGG
TCCTCGCGGTTACATCGATTATCCTATGGTTGATGAGGGCTAG

Upstream 100 bases:

>100_bases
ACTGCTCCTGTATAGGCAGGCGATCTAACGAGACGGAAATATTATCCGAGTGCTCTTCGTTGTACCTTCAGCAAGACAAC
ATACTAACTGAAGGACAAAA

Downstream 100 bases:

>100_bases
ATCTCTTGGCGAAGAAATCTTGCGGCCATCCGGCATCGAGCTTTATATGATGCCTCCATGCAAAATGAGGGTGTCTGATG
GTTTGTGTGTGGGATTCCAT

Product: putative NAD(P)H-dependent reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTTLFESATAGVLELNNRITMAPLTRSRAGQDGVPTDMHVEYYKQRANNGLIVTEGTFFEDINRAFLGQPGIATADQQEG
WRKVAQAVHEQGSNIVMQIMHGGRLTLPSINGFDQGESSSAIAPGVTLHGDGESREEVPTPVALDAEGIKRVIQKFTEGA
RRAIDAGMDGVEVHGANGYLLHQFLAPSSNDRTDEYGGSPENRARLTIEVIRSIADEIGADRVGLRISPMHNIQGCIEED
EDDVRATYKALLDGIADLNIAYLSILYQDAESDLVAYLRESFGGFTILNTGFGAVTELEDARYIVENGLADAVAVGREII
ANPDLSYRWKNNLALNSIDFDTFYTPGPRGYIDYPMVDEG

Sequences:

>Translated_360_residues
MTTLFESATAGVLELNNRITMAPLTRSRAGQDGVPTDMHVEYYKQRANNGLIVTEGTFFEDINRAFLGQPGIATADQQEG
WRKVAQAVHEQGSNIVMQIMHGGRLTLPSINGFDQGESSSAIAPGVTLHGDGESREEVPTPVALDAEGIKRVIQKFTEGA
RRAIDAGMDGVEVHGANGYLLHQFLAPSSNDRTDEYGGSPENRARLTIEVIRSIADEIGADRVGLRISPMHNIQGCIEED
EDDVRATYKALLDGIADLNIAYLSILYQDAESDLVAYLRESFGGFTILNTGFGAVTELEDARYIVENGLADAVAVGREII
ANPDLSYRWKNNLALNSIDFDTFYTPGPRGYIDYPMVDEG
>Mature_359_residues
TTLFESATAGVLELNNRITMAPLTRSRAGQDGVPTDMHVEYYKQRANNGLIVTEGTFFEDINRAFLGQPGIATADQQEGW
RKVAQAVHEQGSNIVMQIMHGGRLTLPSINGFDQGESSSAIAPGVTLHGDGESREEVPTPVALDAEGIKRVIQKFTEGAR
RAIDAGMDGVEVHGANGYLLHQFLAPSSNDRTDEYGGSPENRARLTIEVIRSIADEIGADRVGLRISPMHNIQGCIEEDE
DDVRATYKALLDGIADLNIAYLSILYQDAESDLVAYLRESFGGFTILNTGFGAVTELEDARYIVENGLADAVAVGREIIA
NPDLSYRWKNNLALNSIDFDTFYTPGPRGYIDYPMVDEG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=39.0243902439024, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1789463, Length=229, Percent_Identity=30.1310043668122, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=359, Percent_Identity=29.2479108635098, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=360, Percent_Identity=28.0555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=354, Percent_Identity=29.3785310734463, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=345, Percent_Identity=27.536231884058, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=349, Percent_Identity=27.2206303724928, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=218, Percent_Identity=31.651376146789, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=346, Percent_Identity=25.7225433526012, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=34.4559585492228, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325086, Length=392, Percent_Identity=33.1632653061224, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39248; Mature: 39117

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLFESATAGVLELNNRITMAPLTRSRAGQDGVPTDMHVEYYKQRANNGLIVTEGTFFE
CCCCCCCCCCCEEEECCEEEECCCHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHH
DINRAFLGQPGIATADQQEGWRKVAQAVHEQGSNIVMQIMHGGRLTLPSINGFDQGESSS
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCC
AIAPGVTLHGDGESREEVPTPVALDAEGIKRVIQKFTEGARRAIDAGMDGVEVHGANGYL
CCCCCEEEECCCCCHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
LHQFLAPSSNDRTDEYGGSPENRARLTIEVIRSIADEIGADRVGLRISPMHNIQGCIEED
EHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCC
EDDVRATYKALLDGIADLNIAYLSILYQDAESDLVAYLRESFGGFTILNTGFGAVTELED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
ARYIVENGLADAVAVGREIIANPDLSYRWKNNLALNSIDFDTFYTPGPRGYIDYPMVDEG
HHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTLFESATAGVLELNNRITMAPLTRSRAGQDGVPTDMHVEYYKQRANNGLIVTEGTFFE
CCCCCCCCCCEEEECCEEEECCCHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHH
DINRAFLGQPGIATADQQEGWRKVAQAVHEQGSNIVMQIMHGGRLTLPSINGFDQGESSS
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCC
AIAPGVTLHGDGESREEVPTPVALDAEGIKRVIQKFTEGARRAIDAGMDGVEVHGANGYL
CCCCCEEEECCCCCHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
LHQFLAPSSNDRTDEYGGSPENRARLTIEVIRSIADEIGADRVGLRISPMHNIQGCIEED
EHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCC
EDDVRATYKALLDGIADLNIAYLSILYQDAESDLVAYLRESFGGFTILNTGFGAVTELED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
ARYIVENGLADAVAVGREIIANPDLSYRWKNNLALNSIDFDTFYTPGPRGYIDYPMVDEG
HHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]