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The map label for this gene is dacB [H]

Identifier: 38234569

GI number: 38234569

Start: 2053092

End: 2054372

Strand: Reverse

Name: dacB [H]

Synonym: DIP2005

Alternate gene names: 38234569

Gene position: 2054372-2053092 (Counterclockwise)

Preceding gene: 38234574

Following gene: 38234568

Centisome position: 82.55

GC content: 60.11

Gene sequence:

>1281_bases
GTGCTTGATATTAGCAGTAGGCTGGTTGCGATGAATGGCACAAAGTGGTGGGCAGCGACGATCGCTGCAACAGTAGTGGT
AGCAGGCACCGTGACAACGGGAGCCGTAGTACAACACAAACACGCCTTAACACACCAAGCCCCCTATAAACCCATCGAGC
CTGCTCGCGGCATCACCGCGCTGACAGACAAAGGGACAACCAACAACGAGGCGCTACGCAGCACCGTCGGTGCACTCGCA
CAAGACCCCAGGCTTGCAGGATTCGCCGGCAAAATTGTCGACGCCACCACCGGTGAAGTGATCCTAGAAAAAGACGCCGA
CAAACCACTGACCCCAGCCTCGGCAACCAAAATGCTCACTGCCACCGCGGCACTCTACGAACTAGGCCCCGAACACCGCA
TCACCACCGAAGTGATCAAACAAGACGACCACACCGTGGTTATCAAAGCCGCAGGAGACGTCTGGCTTACCGACGAACGC
CTCGACCAACTAGCACGCAAAATCAAAGAACAAATGCCCAGCGTGAACACAGTCCTCGTAGACACCTCCGCATGGAGTGG
CGACAAAATACTAGGCGGATGGGACCCCGACGACGTCAACGCCGGCTACGTAGCACCCCTCGAACCCATCATGCTCTACG
GCGGACGCCTCGGCGCCAAAGAAGGCGACGTACCACGCAGCCACAACCCAGGACTCGACGTAGCCGCCGCACTCGCACAA
CGCCTCGGTGCAGGAACCAAGGGCTACACCGACTCCGCCACCGGCGAAGTCGTCGCCACCACAGAATCCGAAGCCCTAGA
AAAACGCATCTCCCAAATGATGCAACACTCCGACAACGTGATGGCAGAAGCCATCGGCCGCGAAATCGACGCCACCCAAC
CCGTACAAGCAACACTGCGGATCCTCAGCGAACACGGCATCACCATCGGCGCCTCCGAAGTAGCAGACAACTCAGGGCTC
TCCCTCCACAACCGCATCACCCCAGCGCTACTCACCCAAGTAGCCACCAAGGCCGTAACCGACCCCGAAGTCCACTCGCT
CATCCTCGCCCTGCCCGTCGCAGGCGGCAGCGGAACACTCGCAGACCGCTACGCAGAACTCGACGGCCGCGGCTGGGTAC
GCGCCAAAACCGGAACCCTCACAGGAGTATCGGCGCTGGTCGGCGTGGTGCCGTCGACAAGCGGACACATCTACAGCTTC
GGCCTCATCTCCAACGGCTCCGAAATCCTCGAAGCCCGCGCAGCCCTCGACACCATCGCCTCGGCACTTCGTGATGCATA
A

Upstream 100 bases:

>100_bases
CTCGTGGTCGACTTCGTACTTGTTGCGGGAACCCTTAGGGATCTCGATGGTGACTTCAATGCTCATTGCATTCTCCTCAT
TGACTATAAGAAATAAGACT

Downstream 100 bases:

>100_bases
ATTCTGGCCCCGCCACAGCCCCAACTTCATGGCCTGCCGCGTCGCCGTACGCACAGTACAACCCCGCGACATCACCGTCG
GCCTCTCCGGCGGCGCAGAT

Product: putative D-Ala D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLDISSRLVAMNGTKWWAATIAATVVVAGTVTTGAVVQHKHALTHQAPYKPIEPARGITALTDKGTTNNEALRSTVGALA
QDPRLAGFAGKIVDATTGEVILEKDADKPLTPASATKMLTATAALYELGPEHRITTEVIKQDDHTVVIKAAGDVWLTDER
LDQLARKIKEQMPSVNTVLVDTSAWSGDKILGGWDPDDVNAGYVAPLEPIMLYGGRLGAKEGDVPRSHNPGLDVAAALAQ
RLGAGTKGYTDSATGEVVATTESEALEKRISQMMQHSDNVMAEAIGREIDATQPVQATLRILSEHGITIGASEVADNSGL
SLHNRITPALLTQVATKAVTDPEVHSLILALPVAGGSGTLADRYAELDGRGWVRAKTGTLTGVSALVGVVPSTSGHIYSF
GLISNGSEILEARAALDTIASALRDA

Sequences:

>Translated_426_residues
MLDISSRLVAMNGTKWWAATIAATVVVAGTVTTGAVVQHKHALTHQAPYKPIEPARGITALTDKGTTNNEALRSTVGALA
QDPRLAGFAGKIVDATTGEVILEKDADKPLTPASATKMLTATAALYELGPEHRITTEVIKQDDHTVVIKAAGDVWLTDER
LDQLARKIKEQMPSVNTVLVDTSAWSGDKILGGWDPDDVNAGYVAPLEPIMLYGGRLGAKEGDVPRSHNPGLDVAAALAQ
RLGAGTKGYTDSATGEVVATTESEALEKRISQMMQHSDNVMAEAIGREIDATQPVQATLRILSEHGITIGASEVADNSGL
SLHNRITPALLTQVATKAVTDPEVHSLILALPVAGGSGTLADRYAELDGRGWVRAKTGTLTGVSALVGVVPSTSGHIYSF
GLISNGSEILEARAALDTIASALRDA
>Mature_426_residues
MLDISSRLVAMNGTKWWAATIAATVVVAGTVTTGAVVQHKHALTHQAPYKPIEPARGITALTDKGTTNNEALRSTVGALA
QDPRLAGFAGKIVDATTGEVILEKDADKPLTPASATKMLTATAALYELGPEHRITTEVIKQDDHTVVIKAAGDVWLTDER
LDQLARKIKEQMPSVNTVLVDTSAWSGDKILGGWDPDDVNAGYVAPLEPIMLYGGRLGAKEGDVPRSHNPGLDVAAALAQ
RLGAGTKGYTDSATGEVVATTESEALEKRISQMMQHSDNVMAEAIGREIDATQPVQATLRILSEHGITIGASEVADNSGL
SLHNRITPALLTQVATKAVTDPEVHSLILALPVAGGSGTLADRYAELDGRGWVRAKTGTLTGVSALVGVVPSTSGHIYSF
GLISNGSEILEARAALDTIASALRDA

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=160, Percent_Identity=36.875, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44576; Mature: 44576

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDISSRLVAMNGTKWWAATIAATVVVAGTVTTGAVVQHKHALTHQAPYKPIEPARGITA
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LTDKGTTNNEALRSTVGALAQDPRLAGFAGKIVDATTGEVILEKDADKPLTPASATKMLT
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHH
ATAALYELGPEHRITTEVIKQDDHTVVIKAAGDVWLTDERLDQLARKIKEQMPSVNTVLV
HHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCEEECHHHHHHHHHHHHHHCCCCEEEEE
DTSAWSGDKILGGWDPDDVNAGYVAPLEPIMLYGGRLGAKEGDVPRSHNPGLDVAAALAQ
ECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLGAGTKGYTDSATGEVVATTESEALEKRISQMMQHSDNVMAEAIGREIDATQPVQATLR
HHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHH
ILSEHGITIGASEVADNSGLSLHNRITPALLTQVATKAVTDPEVHSLILALPVAGGSGTL
HHHHCCCEEEHHHHCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCH
ADRYAELDGRGWVRAKTGTLTGVSALVGVVPSTSGHIYSFGLISNGSEILEARAALDTIA
HHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
SALRDA
HHHHCC
>Mature Secondary Structure
MLDISSRLVAMNGTKWWAATIAATVVVAGTVTTGAVVQHKHALTHQAPYKPIEPARGITA
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LTDKGTTNNEALRSTVGALAQDPRLAGFAGKIVDATTGEVILEKDADKPLTPASATKMLT
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHH
ATAALYELGPEHRITTEVIKQDDHTVVIKAAGDVWLTDERLDQLARKIKEQMPSVNTVLV
HHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCEEECHHHHHHHHHHHHHHCCCCEEEEE
DTSAWSGDKILGGWDPDDVNAGYVAPLEPIMLYGGRLGAKEGDVPRSHNPGLDVAAALAQ
ECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLGAGTKGYTDSATGEVVATTESEALEKRISQMMQHSDNVMAEAIGREIDATQPVQATLR
HHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHH
ILSEHGITIGASEVADNSGLSLHNRITPALLTQVATKAVTDPEVHSLILALPVAGGSGTL
HHHHCCCEEEHHHHCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCH
ADRYAELDGRGWVRAKTGTLTGVSALVGVVPSTSGHIYSFGLISNGSEILEARAALDTIA
HHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
SALRDA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]