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The map label for this gene is tilS

Identifier: 38234568

GI number: 38234568

Start: 2052221

End: 2053063

Strand: Reverse

Name: tilS

Synonym: DIP2004

Alternate gene names: 38234568

Gene position: 2053063-2052221 (Counterclockwise)

Preceding gene: 38234569

Following gene: 38234567

Centisome position: 82.5

GC content: 63.58

Gene sequence:

>843_bases
ATGGCCTGCCGCGTCGCCGTACGCACAGTACAACCCCGCGACATCACCGTCGGCCTCTCCGGCGGCGCAGATTCCCTCGC
TCTCGTCGCAGCACTATGCGCCGAAGACTACAACGTCCACGCCCTCTGCGTAGACCACGGACTCCAACCCGGCTCCCACG
AAGTAGCACAACAAGCAGCCCACCACGCCCGAACCTTCGGAGCACACGCCGAAATCATCAGCGTCACCGTCGCGCCGGGC
AACCTCGAAGCCAACGCCCGAACCGCCCGCTACCAAGCACTCCACTCACGCAACCTCCCCGTCGTTGTCGGCCACACCGC
CGACGACCAAGCTGAAACACTCCTGCTCGGCGCGCTACGCGGGAAAGCCACAGGAATGCAGATCCACACCGACAACCTCT
GGCGGCCACTACTTGGCGTTCGCCGCGCCACCACCCTCGCCGCCTGCACAGAACTTGGAATCGAACCCTGGCACGACCCC
CACAACGCCAACACCGACTTCCTGCGCGTAGCACTACGCACCCAAGTCGTGCCGCTGTTGTCCGACATCATTGGTGGCGA
CGCAGTGCCAGCATTGTCACAAGCCGCCACCTTGATCGCCGAAGACACTCACGCCCTGCACGTAGACACCCCCGACGACC
GAATCGCCCAGCTAGCAGGCATGCCACCAGCGTTACGACGCCGCCACCTCGTAGCACTACTCCAAGCCTACGGGGCGCGC
GTGAGCGCCGCTCACCTGCGTGCCGTCGACGCATTAATAACCAATTGGCACGGCCAAAAGGCCGTTCCAGTGGGAAACGG
GTTGGAAGTGACCCGCAAAGATGGCAGACTTTCGGTTAGCTGA

Upstream 100 bases:

>100_bases
TCCAACGGCTCCGAAATCCTCGAAGCCCGCGCAGCCCTCGACACCATCGCCTCGGCACTTCGTGATGCATAAATTCTGGC
CCCGCCACAGCCCCAACTTC

Downstream 100 bases:

>100_bases
TTATGAAAGGTGAAACCCCTATGCACGATTCGAAGGAACTTCCAGTGCCAGCCAACCCCTACGGTAACGACGTGAAAGAA
GTACTGATCACCGAAGATCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MACRVAVRTVQPRDITVGLSGGADSLALVAALCAEDYNVHALCVDHGLQPGSHEVAQQAAHHARTFGAHAEIISVTVAPG
NLEANARTARYQALHSRNLPVVVGHTADDQAETLLLGALRGKATGMQIHTDNLWRPLLGVRRATTLAACTELGIEPWHDP
HNANTDFLRVALRTQVVPLLSDIIGGDAVPALSQAATLIAEDTHALHVDTPDDRIAQLAGMPPALRRRHLVALLQAYGAR
VSAAHLRAVDALITNWHGQKAVPVGNGLEVTRKDGRLSVS

Sequences:

>Translated_280_residues
MACRVAVRTVQPRDITVGLSGGADSLALVAALCAEDYNVHALCVDHGLQPGSHEVAQQAAHHARTFGAHAEIISVTVAPG
NLEANARTARYQALHSRNLPVVVGHTADDQAETLLLGALRGKATGMQIHTDNLWRPLLGVRRATTLAACTELGIEPWHDP
HNANTDFLRVALRTQVVPLLSDIIGGDAVPALSQAATLIAEDTHALHVDTPDDRIAQLAGMPPALRRRHLVALLQAYGAR
VSAAHLRAVDALITNWHGQKAVPVGNGLEVTRKDGRLSVS
>Mature_279_residues
ACRVAVRTVQPRDITVGLSGGADSLALVAALCAEDYNVHALCVDHGLQPGSHEVAQQAAHHARTFGAHAEIISVTVAPGN
LEANARTARYQALHSRNLPVVVGHTADDQAETLLLGALRGKATGMQIHTDNLWRPLLGVRRATTLAACTELGIEPWHDPH
NANTDFLRVALRTQVVPLLSDIIGGDAVPALSQAATLIAEDTHALHVDTPDDRIAQLAGMPPALRRRHLVALLQAYGARV
SAAHLRAVDALITNWHGQKAVPVGNGLEVTRKDGRLSVS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CORDI (Q6NF90)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940335.1
- ProteinModelPortal:   Q6NF90
- SMR:   Q6NF90
- GeneID:   2650108
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2004
- NMPDR:   fig|257309.1.peg.1926
- HOGENOM:   HBG692699
- ProtClustDB:   CLSK633922
- BioCyc:   CDIP257309:DIP2004-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 29716; Mature: 29585

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRVAVRTVQPRDITVGLSGGADSLALVAALCAEDYNVHALCVDHGLQPGSHEVAQQAA
CCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
HHARTFGAHAEIISVTVAPGNLEANARTARYQALHSRNLPVVVGHTADDQAETLLLGALR
HHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHC
GKATGMQIHTDNLWRPLLGVRRATTLAACTELGIEPWHDPHNANTDFLRVALRTQVVPLL
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SDIIGGDAVPALSQAATLIAEDTHALHVDTPDDRIAQLAGMPPALRRRHLVALLQAYGAR
HHHHCCCCCHHHHHHHHHEECCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
VSAAHLRAVDALITNWHGQKAVPVGNGLEVTRKDGRLSVS
HHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCEECC
>Mature Secondary Structure 
ACRVAVRTVQPRDITVGLSGGADSLALVAALCAEDYNVHALCVDHGLQPGSHEVAQQAA
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
HHARTFGAHAEIISVTVAPGNLEANARTARYQALHSRNLPVVVGHTADDQAETLLLGALR
HHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHC
GKATGMQIHTDNLWRPLLGVRRATTLAACTELGIEPWHDPHNANTDFLRVALRTQVVPLL
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SDIIGGDAVPALSQAATLIAEDTHALHVDTPDDRIAQLAGMPPALRRRHLVALLQAYGAR
HHHHCCCCCHHHHHHHHHEECCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
VSAAHLRAVDALITNWHGQKAVPVGNGLEVTRKDGRLSVS
HHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910