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The map label for this gene is ispD

Identifier: 38234537

GI number: 38234537

Start: 2020785

End: 2021519

Strand: Reverse

Name: ispD

Synonym: DIP1973

Alternate gene names: 38234537

Gene position: 2021519-2020785 (Counterclockwise)

Preceding gene: 38234538

Following gene: 38234536

Centisome position: 81.23

GC content: 57.96

Gene sequence:

>735_bases
ATGTCGCGCAGAGTTGTAGCCCTCGTGGCGGCAGCGGGTAAAGGCACTCGGCTGGGTGCAGATCTGCCCAAGGCCTACGT
CCCATTGCGGGACATTCCACTGGTGGTGCGCTCTGTGCGTGCGATGATTACCTCTGCGGTAGTTGACGAGGTCATCGTGA
TTATCAGCCCCCAAATGCAGGATTATGCTGCCGCGCTGTTGCTGCGTTGGGGGCTTTTTAGTGCCGACATCCCTGTGCGT
CTTGTCCATGGCGGTGCCGAGCGCGCCGACTCCGTGTGGTGCGGTTTGCAGGCTATCGCTGATACCGACGCGGTGGTGCT
CATCCATGATTCAGCACGCGCGCTCACCCCACCTGGCATGATTGCCCGAGTTGCACGTGCGGTACTCGACGGTAACAAGG
CTGTTATTCCTGTTGTGCCGGTTGCCGACACCATCAAAACAGTCCACGGCACCACCGTTGTAGCAACGCCTCCACGGAGA
GACTTGCGTGCGGTCCAAACCCCACAAGGTTTCGATATTGCGGCATTGCGGGCAGCCAATATTGCATATCAGCAGCAACA
ACCCGATTTTGAAGCGACCGACGATGCCAGCCTCATGGAGTGGTTTGGCACCCCAGTGCTGTGCGTTGATGGCGACCCCA
TGGCCTTTAAGGTGACTACGCCGCTTGATTATGCGCTGGCAAAAGCCGTGACTGACCAAGCAGAACCAACGATTTTCGAG
GTACCCAGTGACTAA

Upstream 100 bases:

>100_bases
CCGCGCCGAAAACAAGGGCATTGAGGAAACTAAGGTGGAGCCACTGGGCGACATCGACCTCGATGATCTCAATTTCGACG
ACGAAGACTAACCCCACACC

Downstream 100 bases:

>100_bases
TACCCCCATCATCCCGCGTGTGGGCATTGCTACCGACGCACACCAGATCGAGGCCGGAAAACCCTGCTGGATTGCCTGCT
TACTGTTTTCCGGCGCAGAC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSRRVVALVAAAGKGTRLGADLPKAYVPLRDIPLVVRSVRAMITSAVVDEVIVIISPQMQDYAAALLLRWGLFSADIPVR
LVHGGAERADSVWCGLQAIADTDAVVLIHDSARALTPPGMIARVARAVLDGNKAVIPVVPVADTIKTVHGTTVVATPPRR
DLRAVQTPQGFDIAALRAANIAYQQQQPDFEATDDASLMEWFGTPVLCVDGDPMAFKVTTPLDYALAKAVTDQAEPTIFE
VPSD

Sequences:

>Translated_244_residues
MSRRVVALVAAAGKGTRLGADLPKAYVPLRDIPLVVRSVRAMITSAVVDEVIVIISPQMQDYAAALLLRWGLFSADIPVR
LVHGGAERADSVWCGLQAIADTDAVVLIHDSARALTPPGMIARVARAVLDGNKAVIPVVPVADTIKTVHGTTVVATPPRR
DLRAVQTPQGFDIAALRAANIAYQQQQPDFEATDDASLMEWFGTPVLCVDGDPMAFKVTTPLDYALAKAVTDQAEPTIFE
VPSD
>Mature_243_residues
SRRVVALVAAAGKGTRLGADLPKAYVPLRDIPLVVRSVRAMITSAVVDEVIVIISPQMQDYAAALLLRWGLFSADIPVRL
VHGGAERADSVWCGLQAIADTDAVVLIHDSARALTPPGMIARVARAVLDGNKAVIPVVPVADTIKTVHGTTVVATPPRRD
LRAVQTPQGFDIAALRAANIAYQQQQPDFEATDDASLMEWFGTPVLCVDGDPMAFKVTTPLDYALAKAVTDQAEPTIFEV
PSD

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=33.4801762114537, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CORDI (Q6NFC1)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940304.1
- ProteinModelPortal:   Q6NFC1
- SMR:   Q6NFC1
- GeneID:   2649020
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1973
- NMPDR:   fig|257309.1.peg.1895
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   Q6NFC1
- ProtClustDB:   PRK00155
- BioCyc:   CDIP257309:DIP1973-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25999; Mature: 25868

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRVVALVAAAGKGTRLGADLPKAYVPLRDIPLVVRSVRAMITSAVVDEVIVIISPQMQ
CCCEEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
DYAAALLLRWGLFSADIPVRLVHGGAERADSVWCGLQAIADTDAVVLIHDSARALTPPGM
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
IARVARAVLDGNKAVIPVVPVADTIKTVHGTTVVATPPRRDLRAVQTPQGFDIAALRAAN
HHHHHHHHHCCCCEEEEEECCHHHHHHHCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHH
IAYQQQQPDFEATDDASLMEWFGTPVLCVDGDPMAFKVTTPLDYALAKAVTDQAEPTIFE
HHHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCEEEEEECCHHHHHHHHHHCCCCCEEEE
VPSD
CCCC
>Mature Secondary Structure 
SRRVVALVAAAGKGTRLGADLPKAYVPLRDIPLVVRSVRAMITSAVVDEVIVIISPQMQ
CCEEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
DYAAALLLRWGLFSADIPVRLVHGGAERADSVWCGLQAIADTDAVVLIHDSARALTPPGM
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
IARVARAVLDGNKAVIPVVPVADTIKTVHGTTVVATPPRRDLRAVQTPQGFDIAALRAAN
HHHHHHHHHCCCCEEEEEECCHHHHHHHCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHH
IAYQQQQPDFEATDDASLMEWFGTPVLCVDGDPMAFKVTTPLDYALAKAVTDQAEPTIFE
HHHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCEEEEEECCHHHHHHHHHHCCCCCEEEE
VPSD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910