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The map label for this gene is ispF

Identifier: 38234536

GI number: 38234536

Start: 2020310

End: 2020792

Strand: Reverse

Name: ispF

Synonym: DIP1972

Alternate gene names: 38234536

Gene position: 2020792-2020310 (Counterclockwise)

Preceding gene: 38234537

Following gene: 38234535

Centisome position: 81.2

GC content: 58.8

Gene sequence:

>483_bases
GTGACTAATACCCCCATCATCCCGCGTGTGGGCATTGCTACCGACGCACACCAGATCGAGGCCGGAAAACCCTGCTGGAT
TGCCTGCTTACTGTTTTCCGGCGCAGACGGTTGTGAAGGCCACAGTGATGGTGACGTGGTAGCGCACGCGCTTGTCGACG
CCCTCTTGTCCGCCGCTAATCTTGGTGACCTAGGCTCCTTTGTGGGCGTTGGCCGCAAGGAATACGATGGCGTGTCTGGT
ACCCAGTTGCTCACCGAGTGCCGCGAGTTGTTGGAATCCCATGGTTTTACCATCGGCAACGGTGCCGTTCAGCTGGTTGG
GCAATCTCCCAAAATGGGGCCACGGCGGGAAGAAGCCGAGCGCGTGATGAGCGAAATTCTTGGTGCGCCAGTGTCTGTTT
CGGCAACAACAACCGACCATCTTGGTTTTACCGGTCGCAGTGAGGGTAGGGCGGCCGTTGCCACGGCTGTGGTGTGGAAG
TAG

Upstream 100 bases:

>100_bases
GATGGCGACCCCATGGCCTTTAAGGTGACTACGCCGCTTGATTATGCGCTGGCAAAAGCCGTGACTGACCAAGCAGAACC
AACGATTTTCGAGGTACCCA

Downstream 100 bases:

>100_bases
CCTGCTAGACTAACCTGCGTGACTTTACGCATCTTTGACACAGGAACACGCTCGCTTCGAGATTTTGAGCCGATTCGTCC
CGGCCATGCCTCGATCTACC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTNTPIIPRVGIATDAHQIEAGKPCWIACLLFSGADGCEGHSDGDVVAHALVDALLSAANLGDLGSFVGVGRKEYDGVSG
TQLLTECRELLESHGFTIGNGAVQLVGQSPKMGPRREEAERVMSEILGAPVSVSATTTDHLGFTGRSEGRAAVATAVVWK

Sequences:

>Translated_160_residues
MTNTPIIPRVGIATDAHQIEAGKPCWIACLLFSGADGCEGHSDGDVVAHALVDALLSAANLGDLGSFVGVGRKEYDGVSG
TQLLTECRELLESHGFTIGNGAVQLVGQSPKMGPRREEAERVMSEILGAPVSVSATTTDHLGFTGRSEGRAAVATAVVWK
>Mature_159_residues
TNTPIIPRVGIATDAHQIEAGKPCWIACLLFSGADGCEGHSDGDVVAHALVDALLSAANLGDLGSFVGVGRKEYDGVSGT
QLLTECRELLESHGFTIGNGAVQLVGQSPKMGPRREEAERVMSEILGAPVSVSATTTDHLGFTGRSEGRAAVATAVVWK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=39.3548387096774, Blast_Score=96, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CORDI (Q6NFC2)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940303.1
- ProteinModelPortal:   Q6NFC2
- SMR:   Q6NFC2
- GeneID:   2649019
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1972
- NMPDR:   fig|257309.1.peg.1894
- HOGENOM:   HBG630070
- OMA:   VAAHALI
- ProtClustDB:   PRK00084
- BioCyc:   CDIP257309:DIP1972-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16586; Mature: 16455

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTPIIPRVGIATDAHQIEAGKPCWIACLLFSGADGCEGHSDGDVVAHALVDALLSAAN
CCCCCCCCCCCCCCCCHHCCCCCCCEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LGDLGSFVGVGRKEYDGVSGTQLLTECRELLESHGFTIGNGAVQLVGQSPKMGPRREEAE
CCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCHHHHH
RVMSEILGAPVSVSATTTDHLGFTGRSEGRAAVATAVVWK
HHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHEEEECC
>Mature Secondary Structure 
TNTPIIPRVGIATDAHQIEAGKPCWIACLLFSGADGCEGHSDGDVVAHALVDALLSAAN
CCCCCCCCCCCCCCCHHCCCCCCCEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LGDLGSFVGVGRKEYDGVSGTQLLTECRELLESHGFTIGNGAVQLVGQSPKMGPRREEAE
CCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCHHHHH
RVMSEILGAPVSVSATTTDHLGFTGRSEGRAAVATAVVWK
HHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910