Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 38234535

GI number: 38234535

Start: 2018900

End: 2020291

Strand: Reverse

Name: cysS

Synonym: DIP1971

Alternate gene names: 38234535

Gene position: 2020291-2018900 (Counterclockwise)

Preceding gene: 38234536

Following gene: 38234534

Centisome position: 81.18

GC content: 58.41

Gene sequence:

>1392_bases
GTGACTTTACGCATCTTTGACACAGGAACACGCTCGCTTCGAGATTTTGAGCCGATTCGTCCCGGCCATGCCTCGATCTA
CCTGTGTGGAGCAACCCCGCAGACACAACCCCACATTGGGCACGTGCGCTCAGGGGTTGCTTTTGACATTCTGCGTCGTT
GGCTGATTGCCCAAGGCCTCGATGTCGCATTCGTTCGCAACGTCACCGATATTGACGACAAAATCCTCACCAAAGCAGCA
GAAAACGGTCGCCCATGGTGGGAGTGGGTGTCTACCTATGAACGCGAATTTACTTGGGCGTATAACCAGCTCGGTGTGCT
GCCGCCTTCAGTAGAGCCCCGCGCAACCGGACATGTCACCCAGATGGTGGAGTACATGCAACGCCTGATCGATAACGGCT
TTGCTTACGCCGCCGAAGGCTCCGTGTACTTCGACGTGGCAGCATGGACCGCTGCTGAAGGCTCGGACTACGGCAGCTTG
TCGGGCAACCGAGTAGAAGAGATGGAACAGGGCGAAACAGAGATCACCGGCAAACGCGGTGCCCACGACTTCGCACTGTG
GAAGGCCGCAAAACCAGGGGAACCCTCGTGGCCGACCCCGTGGGGTGATGGTCGCCCAGGCTGGCACCTAGAGTGCTCCG
CAATGGCAACCTGGTACCTCGGCGGCGAATTCGATATCCACTGCGGTGGCCTTGATCTTCAGTTCCCACACCACGAGAAC
GAGATCGCCCAATCCCATGCCGCAGGCGATGGTTTTGCCAACTACTGGATGCACAACCACTGGGTCACCATGAGTGGCGA
GAAGATGAGTAAGTCCCTGGGCAACGTCTTGTCCGTTCCCAACATTTTGGACAAAGTCCGCCCAGTCGAGCTGCGCTACT
ACTTGGGGTCTGCACACTACCGCTCCATGCTGGAATACTCCGAAGGTGCACTGCTGGAAGCCGCCGCCGGCTATCGACGC
ATCGAAGCATTCTTAACCAAGGTTGCCGACGTAGAAGCAGGGCAGTGGACCACAGAATTTGAAGAGGCCATGAACGATGA
CCTCGCCGTCCCACGCGCACTGGCAGAGATCCACACCCAAGTCCGGCATGGAAACTCCGCACTTGCAGCAGGAGACGTCG
ATAAGGCCCACGAGATCGCCGCAAGCGTTCGCGCTATGGCCGCCGTGCTAGGCGTTGATCCACTCGACGAGAAGTGGCTT
GATTCCGCAGCCTCCGACTCCTCCACAAACGCCGCACTCGACGTATTGGTACAAGCTGAGCTACAGCGACGCACCGAAGC
ACGCGCTGCCAAGGACTGGGCAACTGCTGATGAAGTCCGCGACCGCCTCCACGCCGCAGGCATCACCGTCACCGATACAC
CTGATGGCCCAACATGGGCACTGAATAACTAA

Upstream 100 bases:

>100_bases
GGCAACAACAACCGACCATCTTGGTTTTACCGGTCGCAGTGAGGGTAGGGCGGCCGTTGCCACGGCTGTGGTGTGGAAGT
AGCCTGCTAGACTAACCTGC

Downstream 100 bases:

>100_bases
ACGACTACAAGAACTGAGAGAACAACCATGGCTGGTAATCACACCAACCGCCCAGGTTTGCGCAAAACCAACAAAAAGGG
CGCAACTAAAGGCTCCGGCG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTLRIFDTGTRSLRDFEPIRPGHASIYLCGATPQTQPHIGHVRSGVAFDILRRWLIAQGLDVAFVRNVTDIDDKILTKAA
ENGRPWWEWVSTYEREFTWAYNQLGVLPPSVEPRATGHVTQMVEYMQRLIDNGFAYAAEGSVYFDVAAWTAAEGSDYGSL
SGNRVEEMEQGETEITGKRGAHDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATWYLGGEFDIHCGGLDLQFPHHEN
EIAQSHAAGDGFANYWMHNHWVTMSGEKMSKSLGNVLSVPNILDKVRPVELRYYLGSAHYRSMLEYSEGALLEAAAGYRR
IEAFLTKVADVEAGQWTTEFEEAMNDDLAVPRALAEIHTQVRHGNSALAAGDVDKAHEIAASVRAMAAVLGVDPLDEKWL
DSAASDSSTNAALDVLVQAELQRRTEARAAKDWATADEVRDRLHAAGITVTDTPDGPTWALNN

Sequences:

>Translated_463_residues
MTLRIFDTGTRSLRDFEPIRPGHASIYLCGATPQTQPHIGHVRSGVAFDILRRWLIAQGLDVAFVRNVTDIDDKILTKAA
ENGRPWWEWVSTYEREFTWAYNQLGVLPPSVEPRATGHVTQMVEYMQRLIDNGFAYAAEGSVYFDVAAWTAAEGSDYGSL
SGNRVEEMEQGETEITGKRGAHDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATWYLGGEFDIHCGGLDLQFPHHEN
EIAQSHAAGDGFANYWMHNHWVTMSGEKMSKSLGNVLSVPNILDKVRPVELRYYLGSAHYRSMLEYSEGALLEAAAGYRR
IEAFLTKVADVEAGQWTTEFEEAMNDDLAVPRALAEIHTQVRHGNSALAAGDVDKAHEIAASVRAMAAVLGVDPLDEKWL
DSAASDSSTNAALDVLVQAELQRRTEARAAKDWATADEVRDRLHAAGITVTDTPDGPTWALNN
>Mature_462_residues
TLRIFDTGTRSLRDFEPIRPGHASIYLCGATPQTQPHIGHVRSGVAFDILRRWLIAQGLDVAFVRNVTDIDDKILTKAAE
NGRPWWEWVSTYEREFTWAYNQLGVLPPSVEPRATGHVTQMVEYMQRLIDNGFAYAAEGSVYFDVAAWTAAEGSDYGSLS
GNRVEEMEQGETEITGKRGAHDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATWYLGGEFDIHCGGLDLQFPHHENE
IAQSHAAGDGFANYWMHNHWVTMSGEKMSKSLGNVLSVPNILDKVRPVELRYYLGSAHYRSMLEYSEGALLEAAAGYRRI
EAFLTKVADVEAGQWTTEFEEAMNDDLAVPRALAEIHTQVRHGNSALAAGDVDKAHEIAASVRAMAAVLGVDPLDEKWLD
SAASDSSTNAALDVLVQAELQRRTEARAAKDWATADEVRDRLHAAGITVTDTPDGPTWALNN

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=463, Percent_Identity=35.2051835853132, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI62240992, Length=298, Percent_Identity=38.9261744966443, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI303304987, Length=298, Percent_Identity=38.9261744966443, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI21269877, Length=298, Percent_Identity=38.9261744966443, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI10835051, Length=298, Percent_Identity=38.9261744966443, Blast_Score=212, Evalue=8e-55,
Organism=Escherichia coli, GI1786737, Length=467, Percent_Identity=44.1113490364026, Blast_Score=342, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17509795, Length=370, Percent_Identity=31.3513513513514, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17509797, Length=370, Percent_Identity=31.3513513513514, Blast_Score=195, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6324082, Length=370, Percent_Identity=33.2432432432432, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19922218, Length=504, Percent_Identity=33.7301587301587, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19922364, Length=269, Percent_Identity=40.5204460966543, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CORDI (Q6NFC3)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940302.1
- ProteinModelPortal:   Q6NFC3
- SMR:   Q6NFC3
- GeneID:   2649018
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1971
- NMPDR:   fig|257309.1.peg.1893
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q6NFC3
- ProtClustDB:   PRK00260
- BioCyc:   CDIP257309:DIP1971-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51195; Mature: 51064

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRIFDTGTRSLRDFEPIRPGHASIYLCGATPQTQPHIGHVRSGVAFDILRRWLIAQGL
CEEEEEECCCCCHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKAAENGRPWWEWVSTYEREFTWAYNQLGVLPPSVEPRATGHVT
CEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QMVEYMQRLIDNGFAYAAEGSVYFDVAAWTAAEGSDYGSLSGNRVEEMEQGETEITGKRG
HHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEECCCCC
AHDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATWYLGGEFDIHCGGLDLQFPHHEN
CCHHHHEECCCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCEEEEEECCEEEECCCCHH
EIAQSHAAGDGFANYWMHNHWVTMSGEKMSKSLGNVLSVPNILDKVRPVELRYYLGSAHY
HHHHHHCCCCCHHHHEEECCEEEECCHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCHHH
RSMLEYSEGALLEAAAGYRRIEAFLTKVADVEAGQWTTEFEEAMNDDLAVPRALAEIHTQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
VRHGNSALAAGDVDKAHEIAASVRAMAAVLGVDPLDEKWLDSAASDSSTNAALDVLVQAE
HHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
LQRRTEARAAKDWATADEVRDRLHAAGITVTDTPDGPTWALNN
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
TLRIFDTGTRSLRDFEPIRPGHASIYLCGATPQTQPHIGHVRSGVAFDILRRWLIAQGL
EEEEEECCCCCHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKAAENGRPWWEWVSTYEREFTWAYNQLGVLPPSVEPRATGHVT
CEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QMVEYMQRLIDNGFAYAAEGSVYFDVAAWTAAEGSDYGSLSGNRVEEMEQGETEITGKRG
HHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEECCCCC
AHDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATWYLGGEFDIHCGGLDLQFPHHEN
CCHHHHEECCCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCEEEEEECCEEEECCCCHH
EIAQSHAAGDGFANYWMHNHWVTMSGEKMSKSLGNVLSVPNILDKVRPVELRYYLGSAHY
HHHHHHCCCCCHHHHEEECCEEEECCHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCHHH
RSMLEYSEGALLEAAAGYRRIEAFLTKVADVEAGQWTTEFEEAMNDDLAVPRALAEIHTQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
VRHGNSALAAGDVDKAHEIAASVRAMAAVLGVDPLDEKWLDSAASDSSTNAALDVLVQAE
HHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
LQRRTEARAAKDWATADEVRDRLHAAGITVTDTPDGPTWALNN
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910