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The map label for this gene is otsA [H]

Identifier: 38234530

GI number: 38234530

Start: 2014122

End: 2015561

Strand: Direct

Name: otsA [H]

Synonym: DIP1966

Alternate gene names: 38234530

Gene position: 2014122-2015561 (Clockwise)

Preceding gene: 38234529

Following gene: 38234531

Centisome position: 80.93

GC content: 53.68

Gene sequence:

>1440_bases
GTGTCTGCGATCGAATCGAATGAGGGCGAGCAGCTTTTCGACTTTGTCGTGGTTGCTAATCGGTTGCCTGTTGACTTAAG
CACGTCTGCTGATGGGTCGCCGCGTTGGGTGGCTAGCCCTGGCGGTTTGGTCACTGCGTTAACGCCTGTTCTTGCTGGCA
ACCGTGGTTGTTGGGTGGGCTGGCATGGCGGTACTGGCCCTGCCCCTGATCCGTTTCGTACTGACACGAACATTTGGGTG
CATCCGGTTGCGTTGAGCGACCATGATTTTGAGGGCTTTTACGAGGGCTTTTCTAATGCCACCCTGTGGCCGTTGTACCA
CGATTTGATTGTGTCGCCGGTGTATAACCGCGATTGGTGGACGGCCTACCGTGAGGTGAATTTGAAGTTTGCTTCGGCGG
TTGCTGAGGTTGCCGCCGAGGGCGCTGTGGTGTGGATTCAGGATTATCAGTTACAGCTGGTACCTGGTATTTTGCGCCAG
ATTCGCCCTGATCTTACGATCGGCTTTTTCTTGCATATCCCGTTCCCGCATTCGGATTTGTTCCGCCAGTTGCCGTGGCG
CGAGGAGATTGTGCGTGGGTTGTTGGGGGCGGATTTGGTTGGTTTCCATCTGACATCCAGCGCAAATAATTTCTTGGATT
TGGCTGCGGCTCTTGGTTTAGAGGCACAGGGCCGTGCGAAGGTCCGCGAGGTTAGTGCTACGGTGACAGCTGGCGACGGC
CATGTGGTGAGCGTGGCGGCGTATCCGATTTCCATTGATGTGGAGCCGTTGATTGCTTCGCATAGTAATCGTGATGAGAT
CCGTGCTGAGTTTGGCTACCCAGAGACGCTGATTTTGGGTGTGGATCGCCTCGATTACACGAAGGGTATTTTGCAGCGTC
TATTGGCCTTTGAGGAGCTGCTGGAAAGCGGTGCTATCGATCCGAATACCACCGTGTTTGCGCAGGTGGCTACGCCTTCG
CGGGAGCGTATCGAGCATTATAAGCAGGCTCGTTCTGAGGTTGAAGAGGCCGTGGGTCGTATTAATGGCCGTTTTGGTTC
CATTGGTCGTTCTGTGGTGCATTATCACCATCGTCACATGGATCGTAGTGAGCTGAATAAGCTGTATCGTGCCGCGGACG
TTATGCTGGTCACGCCGTTTAAAGATGGTATGAATTTGGTGGCCAAGGAGTATGTGTCTTGCCACAGCGATGGTTCAGGC
GCAATGGTTCTTAGCGAGTTCGCTGGTGCCGCCGTGGAGCTTGAGCAGGCATTTTTGTGTAATCCTTTTGATTTGGAGTC
GATCAAGCGCCAGATTGCTGGTGCGGTGACAACGCTGAAGAATCATCCGGAAGCTGCACGAGATCGTATGCTGGCTATGC
ATCAGCAAGTAGTCGACCATGATGTGAATGTGTGGGCTTCGGCGTTTTTGCGTGATCTTAGTAGTGCGCATACGTCGTAA

Upstream 100 bases:

>100_bases
AGAGCACTAAGAAAACCACCAAGAAGTCGACAAAGAAGACCACGAAGAAGGCTGCTAAGAAGAGCACCAAGAAGACCACT
AAAAAGGCCTAGTGGGCGTC

Downstream 100 bases:

>100_bases
AGCGTCCTTCTAGTCTCACGAGGAGTGATGGTGTTGCGTCGATTAGTAGTTGTTGCCTGCATGGGTGCGCTTCTTGCGGG
GTGTGGTACGCAGCCTACTC

Product: putative glycosyltransferase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSAIESNEGEQLFDFVVVANRLPVDLSTSADGSPRWVASPGGLVTALTPVLAGNRGCWVGWHGGTGPAPDPFRTDTNIWV
HPVALSDHDFEGFYEGFSNATLWPLYHDLIVSPVYNRDWWTAYREVNLKFASAVAEVAAEGAVVWIQDYQLQLVPGILRQ
IRPDLTIGFFLHIPFPHSDLFRQLPWREEIVRGLLGADLVGFHLTSSANNFLDLAAALGLEAQGRAKVREVSATVTAGDG
HVVSVAAYPISIDVEPLIASHSNRDEIRAEFGYPETLILGVDRLDYTKGILQRLLAFEELLESGAIDPNTTVFAQVATPS
RERIEHYKQARSEVEEAVGRINGRFGSIGRSVVHYHHRHMDRSELNKLYRAADVMLVTPFKDGMNLVAKEYVSCHSDGSG
AMVLSEFAGAAVELEQAFLCNPFDLESIKRQIAGAVTTLKNHPEAARDRMLAMHQQVVDHDVNVWASAFLRDLSSAHTS

Sequences:

>Translated_479_residues
MSAIESNEGEQLFDFVVVANRLPVDLSTSADGSPRWVASPGGLVTALTPVLAGNRGCWVGWHGGTGPAPDPFRTDTNIWV
HPVALSDHDFEGFYEGFSNATLWPLYHDLIVSPVYNRDWWTAYREVNLKFASAVAEVAAEGAVVWIQDYQLQLVPGILRQ
IRPDLTIGFFLHIPFPHSDLFRQLPWREEIVRGLLGADLVGFHLTSSANNFLDLAAALGLEAQGRAKVREVSATVTAGDG
HVVSVAAYPISIDVEPLIASHSNRDEIRAEFGYPETLILGVDRLDYTKGILQRLLAFEELLESGAIDPNTTVFAQVATPS
RERIEHYKQARSEVEEAVGRINGRFGSIGRSVVHYHHRHMDRSELNKLYRAADVMLVTPFKDGMNLVAKEYVSCHSDGSG
AMVLSEFAGAAVELEQAFLCNPFDLESIKRQIAGAVTTLKNHPEAARDRMLAMHQQVVDHDVNVWASAFLRDLSSAHTS
>Mature_478_residues
SAIESNEGEQLFDFVVVANRLPVDLSTSADGSPRWVASPGGLVTALTPVLAGNRGCWVGWHGGTGPAPDPFRTDTNIWVH
PVALSDHDFEGFYEGFSNATLWPLYHDLIVSPVYNRDWWTAYREVNLKFASAVAEVAAEGAVVWIQDYQLQLVPGILRQI
RPDLTIGFFLHIPFPHSDLFRQLPWREEIVRGLLGADLVGFHLTSSANNFLDLAAALGLEAQGRAKVREVSATVTAGDGH
VVSVAAYPISIDVEPLIASHSNRDEIRAEFGYPETLILGVDRLDYTKGILQRLLAFEELLESGAIDPNTTVFAQVATPSR
ERIEHYKQARSEVEEAVGRINGRFGSIGRSVVHYHHRHMDRSELNKLYRAADVMLVTPFKDGMNLVAKEYVSCHSDGSGA
MVLSEFAGAAVELEQAFLCNPFDLESIKRQIAGAVTTLKNHPEAARDRMLAMHQQVVDHDVNVWASAFLRDLSSAHTS

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=471, Percent_Identity=34.1825902335456, Blast_Score=250, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI115533558, Length=423, Percent_Identity=30.0236406619385, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI115533560, Length=422, Percent_Identity=29.6208530805687, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71987012, Length=430, Percent_Identity=28.3720930232558, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319602, Length=483, Percent_Identity=35.1966873706004, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6323537, Length=405, Percent_Identity=30.8641975308642, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323917, Length=405, Percent_Identity=28.641975308642, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320279, Length=426, Percent_Identity=28.4037558685446, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI19920676, Length=502, Percent_Identity=35.6573705179283, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 52831; Mature: 52700

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIESNEGEQLFDFVVVANRLPVDLSTSADGSPRWVASPGGLVTALTPVLAGNRGCWVG
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHCCCCCEEEE
WHGGTGPAPDPFRTDTNIWVHPVALSDHDFEGFYEGFSNATLWPLYHDLIVSPVYNRDWW
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCEEHHHHHHHHHCCCCCCHHH
TAYREVNLKFASAVAEVAAEGAVVWIQDYQLQLVPGILRQIRPDLTIGFFLHIPFPHSDL
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHH
FRQLPWREEIVRGLLGADLVGFHLTSSANNFLDLAAALGLEAQGRAKVREVSATVTAGDG
HHHCCCHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEEEEECCCC
HVVSVAAYPISIDVEPLIASHSNRDEIRAEFGYPETLILGVDRLDYTKGILQRLLAFEEL
CEEEEEEEEEEECCCHHHCCCCCHHHHHECCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LESGAIDPNTTVFAQVATPSRERIEHYKQARSEVEEAVGRINGRFGSIGRSVVHYHHRHM
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DRSELNKLYRAADVMLVTPFKDGMNLVAKEYVSCHSDGSGAMVLSEFAGAAVELEQAFLC
CHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCC
NPFDLESIKRQIAGAVTTLKNHPEAARDRMLAMHQQVVDHDVNVWASAFLRDLSSAHTS
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAIESNEGEQLFDFVVVANRLPVDLSTSADGSPRWVASPGGLVTALTPVLAGNRGCWVG
CCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHCCCCCEEEE
WHGGTGPAPDPFRTDTNIWVHPVALSDHDFEGFYEGFSNATLWPLYHDLIVSPVYNRDWW
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCEEHHHHHHHHHCCCCCCHHH
TAYREVNLKFASAVAEVAAEGAVVWIQDYQLQLVPGILRQIRPDLTIGFFLHIPFPHSDL
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHH
FRQLPWREEIVRGLLGADLVGFHLTSSANNFLDLAAALGLEAQGRAKVREVSATVTAGDG
HHHCCCHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEEEEECCCC
HVVSVAAYPISIDVEPLIASHSNRDEIRAEFGYPETLILGVDRLDYTKGILQRLLAFEEL
CEEEEEEEEEEECCCHHHCCCCCHHHHHECCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LESGAIDPNTTVFAQVATPSRERIEHYKQARSEVEEAVGRINGRFGSIGRSVVHYHHRHM
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
DRSELNKLYRAADVMLVTPFKDGMNLVAKEYVSCHSDGSGAMVLSEFAGAAVELEQAFLC
CHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCC
NPFDLESIKRQIAGAVTTLKNHPEAARDRMLAMHQQVVDHDVNVWASAFLRDLSSAHTS
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA