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The map label for this gene is yclK [H]

Identifier: 38234502

GI number: 38234502

Start: 1989473

End: 1991032

Strand: Reverse

Name: yclK [H]

Synonym: DIP1935

Alternate gene names: 38234502

Gene position: 1991032-1989473 (Counterclockwise)

Preceding gene: 38234503

Following gene: 38234499

Centisome position: 80.0

GC content: 52.95

Gene sequence:

>1560_bases
ATGAACAATCCTTACGCCAGAGCGGTTGTTTCGGAACCAATAGATGAAACGCAATGTTCATCTGTTGGAGATCACCGGTA
CCCGAATTTTGCCAAGAAAAAACAGGAAGACCACAAGCCCTCCTGGATCAGCACAGTCGTGCGTTCTCCACGAATTTCGG
CTATCAAACATCTACGTTTGAGCGGGATTCCCCTGCGCTCCGGTTTGATGGCGATTGTTCTTGTTGTCTCTGCAATTGGG
CTGATCGGAAGCTCTTTTGCAGTTAATCGAACGATGCAGCAGTTGTCCTATTCTCGCATTGATATGGAACTTGAAAGCGG
ACTCAACGGCTGGGCCGCTCAAGACTCCTTGTTTGAAACGCAGCTTAATGGTGCGCTGGTTCGCCCACCGACAGAATTCT
ATGTGGTGAAGATCTACCCTAATGGGACAACGAGCATCTATAACGAGGGAAAAACGAGCCCTGACCTGGGTCGCGTCGTT
ATCGGCAAAGGGGCGCAAACCGTTGATTCCTCGGAGGAAAGCGCTGCAAACGTGGAATGGCGGGTCATCGCGGAGCGCCG
TGCTGGTGTAACCATTGTAGTGGCCAAGGATATTACTCAAGAAGTAAATGTCTTAAAACGCCTCGCATTAGGCCAGGTCA
TCATTGTTCTAGCAGTGCTTTTGCTCATGGCGTTATTGGCCTATGTGCTGATCCAACGTGCACTTCGGCCATTACGTGAA
GTCGAGATGACCGCCAAAGCGATTGCCGCGGGTGATTTGGACCGCCGAGTGCCCAACACTACGGCAAATACTGAAGTGGG
AGCGTTGTCGAGCGCGCTGAACAGTATGATCTCTCAGCTGCAGGGATCCATTGTGGAGCTGCGGGATAAAGAGGCCCAAA
TGCGTCGTTTCGTTGGCGATGCCTCACATGAGTTGCGAACCCCACTGACCTCGGTCAAAGGCTATGCCGAGCTGTATCGC
TCGGGTGCTACAACGGACGCAGACCTCGTGATTGAAAAGATCGAAGACGAGGCTAAGCGAATGAGTTTGCTGGTAGAAGA
CCTCTTAGCGCTCACGCGAGCAGAGGGGGCACGCCATGATTCCAAGCCTGTTGATCTTCTTGATCTAGCACTGAACGTTT
CGTCTTCACTTCGGGGAGCTTATCCCGATCGCGACATCGATGTTCGCTCCGAATGCACGGATGTGCCCGTTGTGGAAGGA
GACGCAGCCCGCCTGCACCAAGTTCTGACCAACTTGGTTGCAAATGCCTTGAAGCACGGCGGAGACTCGGCACGCGTCAC
CATCAAACTAGCCGACGCGGGATCCAATTTTGCCGTCAAAGTGATCGATGACGGCATAGGCCTCAGCGAAGAAGATGCCT
CGCATATCTTCGAACGCTTCTACAGGGCAGACAGTTCGCGCGCCCGCAGCACAGGCGGAAGTGGCCTAGGGCTCGCCATC
GTGAAATCCCTTGTGGAATCCCACGGTGGTGAAGTATCAGTGGAATCTGAACAAGGCCATGGCACCACATTCATCGTGGA
ACTACCCAAAACTGCACCGAAAGAGACTAAAAACCGCTAA

Upstream 100 bases:

>100_bases
AAGAACCAGCGTTGATTCAAACGGTTCGTGGTGTCGGCTACGTGCTACGTAAACCTCGGTCGTAGAAGCAAGGATTCGTC
GATAGGAAATCTAGACAAGC

Downstream 100 bases:

>100_bases
AGCACCCGAGGTACTAGTCGGTAGGGGAGTCGGTTTCCGCAGGGTAACCGAGAGCCTCCATAATGACATCGGCTTGCCGT
GGTAAATCTTCGTGGCGAAT

Product: putative two somponent system sensor kinase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNNPYARAVVSEPIDETQCSSVGDHRYPNFAKKKQEDHKPSWISTVVRSPRISAIKHLRLSGIPLRSGLMAIVLVVSAIG
LIGSSFAVNRTMQQLSYSRIDMELESGLNGWAAQDSLFETQLNGALVRPPTEFYVVKIYPNGTTSIYNEGKTSPDLGRVV
IGKGAQTVDSSEESAANVEWRVIAERRAGVTIVVAKDITQEVNVLKRLALGQVIIVLAVLLLMALLAYVLIQRALRPLRE
VEMTAKAIAAGDLDRRVPNTTANTEVGALSSALNSMISQLQGSIVELRDKEAQMRRFVGDASHELRTPLTSVKGYAELYR
SGATTDADLVIEKIEDEAKRMSLLVEDLLALTRAEGARHDSKPVDLLDLALNVSSSLRGAYPDRDIDVRSECTDVPVVEG
DAARLHQVLTNLVANALKHGGDSARVTIKLADAGSNFAVKVIDDGIGLSEEDASHIFERFYRADSSRARSTGGSGLGLAI
VKSLVESHGGEVSVESEQGHGTTFIVELPKTAPKETKNR

Sequences:

>Translated_519_residues
MNNPYARAVVSEPIDETQCSSVGDHRYPNFAKKKQEDHKPSWISTVVRSPRISAIKHLRLSGIPLRSGLMAIVLVVSAIG
LIGSSFAVNRTMQQLSYSRIDMELESGLNGWAAQDSLFETQLNGALVRPPTEFYVVKIYPNGTTSIYNEGKTSPDLGRVV
IGKGAQTVDSSEESAANVEWRVIAERRAGVTIVVAKDITQEVNVLKRLALGQVIIVLAVLLLMALLAYVLIQRALRPLRE
VEMTAKAIAAGDLDRRVPNTTANTEVGALSSALNSMISQLQGSIVELRDKEAQMRRFVGDASHELRTPLTSVKGYAELYR
SGATTDADLVIEKIEDEAKRMSLLVEDLLALTRAEGARHDSKPVDLLDLALNVSSSLRGAYPDRDIDVRSECTDVPVVEG
DAARLHQVLTNLVANALKHGGDSARVTIKLADAGSNFAVKVIDDGIGLSEEDASHIFERFYRADSSRARSTGGSGLGLAI
VKSLVESHGGEVSVESEQGHGTTFIVELPKTAPKETKNR
>Mature_519_residues
MNNPYARAVVSEPIDETQCSSVGDHRYPNFAKKKQEDHKPSWISTVVRSPRISAIKHLRLSGIPLRSGLMAIVLVVSAIG
LIGSSFAVNRTMQQLSYSRIDMELESGLNGWAAQDSLFETQLNGALVRPPTEFYVVKIYPNGTTSIYNEGKTSPDLGRVV
IGKGAQTVDSSEESAANVEWRVIAERRAGVTIVVAKDITQEVNVLKRLALGQVIIVLAVLLLMALLAYVLIQRALRPLRE
VEMTAKAIAAGDLDRRVPNTTANTEVGALSSALNSMISQLQGSIVELRDKEAQMRRFVGDASHELRTPLTSVKGYAELYR
SGATTDADLVIEKIEDEAKRMSLLVEDLLALTRAEGARHDSKPVDLLDLALNVSSSLRGAYPDRDIDVRSECTDVPVVEG
DAARLHQVLTNLVANALKHGGDSARVTIKLADAGSNFAVKVIDDGIGLSEEDASHIFERFYRADSSRARSTGGSGLGLAI
VKSLVESHGGEVSVESEQGHGTTFIVELPKTAPKETKNR

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=278, Percent_Identity=32.7338129496403, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786783, Length=352, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786600, Length=294, Percent_Identity=30.952380952381, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790346, Length=285, Percent_Identity=30.5263157894737, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789149, Length=345, Percent_Identity=29.2753623188406, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788279, Length=336, Percent_Identity=27.3809523809524, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=30.6034482758621, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=28.3783783783784, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081816, Length=349, Percent_Identity=28.3667621776504, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790551, Length=302, Percent_Identity=30.794701986755, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082128, Length=316, Percent_Identity=26.5822784810127, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790861, Length=244, Percent_Identity=30.7377049180328, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=30.2752293577982, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789403, Length=319, Percent_Identity=30.0940438871473, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789808, Length=295, Percent_Identity=26.4406779661017, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788549, Length=283, Percent_Identity=26.1484098939929, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790300, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787374, Length=315, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56200; Mature: 56200

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPYARAVVSEPIDETQCSSVGDHRYPNFAKKKQEDHKPSWISTVVRSPRISAIKHLRL
CCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
SGIPLRSGLMAIVLVVSAIGLIGSSFAVNRTMQQLSYSRIDMELESGLNGWAAQDSLFET
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QLNGALVRPPTEFYVVKIYPNGTTSIYNEGKTSPDLGRVVIGKGAQTVDSSEESAANVEW
HHCCEEECCCCEEEEEEEECCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCCHHHCCCEE
RVIAERRAGVTIVVAKDITQEVNVLKRLALGQVIIVLAVLLLMALLAYVLIQRALRPLRE
EEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEMTAKAIAAGDLDRRVPNTTANTEVGALSSALNSMISQLQGSIVELRDKEAQMRRFVGD
HHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
ASHELRTPLTSVKGYAELYRSGATTDADLVIEKIEDEAKRMSLLVEDLLALTRAEGARHD
CHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SKPVDLLDLALNVSSSLRGAYPDRDIDVRSECTDVPVVEGDAARLHQVLTNLVANALKHG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GDSARVTIKLADAGSNFAVKVIDDGIGLSEEDASHIFERFYRADSSRARSTGGSGLGLAI
CCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
VKSLVESHGGEVSVESEQGHGTTFIVELPKTAPKETKNR
HHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNNPYARAVVSEPIDETQCSSVGDHRYPNFAKKKQEDHKPSWISTVVRSPRISAIKHLRL
CCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
SGIPLRSGLMAIVLVVSAIGLIGSSFAVNRTMQQLSYSRIDMELESGLNGWAAQDSLFET
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QLNGALVRPPTEFYVVKIYPNGTTSIYNEGKTSPDLGRVVIGKGAQTVDSSEESAANVEW
HHCCEEECCCCEEEEEEEECCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCCHHHCCCEE
RVIAERRAGVTIVVAKDITQEVNVLKRLALGQVIIVLAVLLLMALLAYVLIQRALRPLRE
EEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEMTAKAIAAGDLDRRVPNTTANTEVGALSSALNSMISQLQGSIVELRDKEAQMRRFVGD
HHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
ASHELRTPLTSVKGYAELYRSGATTDADLVIEKIEDEAKRMSLLVEDLLALTRAEGARHD
CHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SKPVDLLDLALNVSSSLRGAYPDRDIDVRSECTDVPVVEGDAARLHQVLTNLVANALKHG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GDSARVTIKLADAGSNFAVKVIDDGIGLSEEDASHIFERFYRADSSRARSTGGSGLGLAI
CCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
VKSLVESHGGEVSVESEQGHGTTFIVELPKTAPKETKNR
HHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]