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The map label for this gene is purD [H]

Identifier: 38234499

GI number: 38234499

Start: 1986987

End: 1988273

Strand: Reverse

Name: purD [H]

Synonym: DIP1932

Alternate gene names: 38234499

Gene position: 1988273-1986987 (Counterclockwise)

Preceding gene: 38234502

Following gene: 38234498

Centisome position: 79.89

GC content: 57.73

Gene sequence:

>1287_bases
ATGCGCATTCTTGTTATCGGTTCGGGCGCCCGTGAGCACGCCCTCGTACTAGGTTTGTCCAAGGATCCCTCTGTTTCTGA
GATCCATGTTGCCCCAGGCAACGCAGGCATGGAGCAGATCGCAACGGTTCATGCTGATGCCACTTCCGTGGATGACCCAG
CACAGATGCTGCAGGTCGCGCAGGATGTTCAGGCTGATTTGGTTGTGGTCGGCCCTGAAATTCCGCTGGTAGCAGGTGTG
GCGGATGTTCTTCGTCAGAACGGTTTCGCGGTGTTCGGACCGAATAAGGATGCTGCTCGCATCGAGGGCTCGAAGGCTTT
TGCCAAAGATGTGATGGAAAAAGCTGGTGTGCGTACGGCTCGTGCGGAGCAGATTCTTCCTGAGGCAAGCGATGAGCAAC
GCGATGCGGCTTTGGATCGTTTTGGCCCGCATTATGTGGTCAAGGATGATGGCCTTGCTGGCGGCAAGGGTGTTGTTGTT
ACTGATGATCGTGAGGCTGCGCGTGCGCATGTCGACGCGGTGCTCGCTGCGGGTAATCCGGTGCTGTTGGAGAGCTTCTT
GGATGGCCCTGAGGTGTCCTTGTTCTGCTTGGTTGATGGCGAGACTGTCGTTCCTTTGATCCCTGCGCAGGATCACAAGC
GTGCTTACGATAATGATGAGGGCCCGAACACGGGTGGTATGGGCGCTTATACGCCGTTGCCGTGGCTTCCTGAAGATGGA
GTACAGCGCATTGTCGACGAAGTCTGTGTGCCTGTGGCTCGTGAGATGGTGCGTCGTGGTTGCTCGTATTCTGGTTTGTT
GTATGCAGGCTTGGCGTGGGGTGCTGAGGGCCCCTCGGTGGTGGAATTTAACTGCCGTTTTGGTGATCCAGAAACTCAGG
CGGTGTTGGCGTTGCTGCGTACCCCGTTGGCTCAGGTGCTGCAGTCGGTGGCTGAGGGTACGTTGGCGCAGGCCCCAGCG
TTGGAGTGGCAGGATGGCTACGCTTTGACTGTTGTTTTGGCTGCTGAGGGGTACCCAGAAAGCCCACGTAAGGGTGGCGT
GATCACGGGTGCTGATGTGGCTGATGCCTCGGGTACCACGGCTGTGTTGCATGCTGGTACTGCACGTGATGAGCAGGGCA
ACGTGGTGGCCAATGGTGGCCGTGTGCTCAACGTGGTGGGTACGGGGGCAACGCTTGCCGACGCTCGCGACAACGCCTAC
CGCGTGTTGGAAGGGATCAAGCTGGAGGGTTCGCATTTCCGCTCTGATATTGCGCTTCCGGCAGTTGAGGGTCGTATTTC
GATTTAA

Upstream 100 bases:

>100_bases
TTTTGGTAAATACGCTACTCATGCTGTCTACGGTACCGCGCAGTGCGCACTATTGTCAGGGGAGGAATGCTGGGCGTAGA
CCCTGTAGGATCTACAGATC

Downstream 100 bases:

>100_bases
GCATTTATACGGCTTGCTGCATGGAGGGCCTAACAGTGCCCCTAGTGTTGTGAGTTGGGGCATATGCGGAGTATCGTCGC
CTCGTGCGTAAGACTCCTGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRILVIGSGAREHALVLGLSKDPSVSEIHVAPGNAGMEQIATVHADATSVDDPAQMLQVAQDVQADLVVVGPEIPLVAGV
ADVLRQNGFAVFGPNKDAARIEGSKAFAKDVMEKAGVRTARAEQILPEASDEQRDAALDRFGPHYVVKDDGLAGGKGVVV
TDDREAARAHVDAVLAAGNPVLLESFLDGPEVSLFCLVDGETVVPLIPAQDHKRAYDNDEGPNTGGMGAYTPLPWLPEDG
VQRIVDEVCVPVAREMVRRGCSYSGLLYAGLAWGAEGPSVVEFNCRFGDPETQAVLALLRTPLAQVLQSVAEGTLAQAPA
LEWQDGYALTVVLAAEGYPESPRKGGVITGADVADASGTTAVLHAGTARDEQGNVVANGGRVLNVVGTGATLADARDNAY
RVLEGIKLEGSHFRSDIALPAVEGRISI

Sequences:

>Translated_428_residues
MRILVIGSGAREHALVLGLSKDPSVSEIHVAPGNAGMEQIATVHADATSVDDPAQMLQVAQDVQADLVVVGPEIPLVAGV
ADVLRQNGFAVFGPNKDAARIEGSKAFAKDVMEKAGVRTARAEQILPEASDEQRDAALDRFGPHYVVKDDGLAGGKGVVV
TDDREAARAHVDAVLAAGNPVLLESFLDGPEVSLFCLVDGETVVPLIPAQDHKRAYDNDEGPNTGGMGAYTPLPWLPEDG
VQRIVDEVCVPVAREMVRRGCSYSGLLYAGLAWGAEGPSVVEFNCRFGDPETQAVLALLRTPLAQVLQSVAEGTLAQAPA
LEWQDGYALTVVLAAEGYPESPRKGGVITGADVADASGTTAVLHAGTARDEQGNVVANGGRVLNVVGTGATLADARDNAY
RVLEGIKLEGSHFRSDIALPAVEGRISI
>Mature_428_residues
MRILVIGSGAREHALVLGLSKDPSVSEIHVAPGNAGMEQIATVHADATSVDDPAQMLQVAQDVQADLVVVGPEIPLVAGV
ADVLRQNGFAVFGPNKDAARIEGSKAFAKDVMEKAGVRTARAEQILPEASDEQRDAALDRFGPHYVVKDDGLAGGKGVVV
TDDREAARAHVDAVLAAGNPVLLESFLDGPEVSLFCLVDGETVVPLIPAQDHKRAYDNDEGPNTGGMGAYTPLPWLPEDG
VQRIVDEVCVPVAREMVRRGCSYSGLLYAGLAWGAEGPSVVEFNCRFGDPETQAVLALLRTPLAQVLQSVAEGTLAQAPA
LEWQDGYALTVVLAAEGYPESPRKGGVITGADVADASGTTAVLHAGTARDEQGNVVANGGRVLNVVGTGATLADARDNAY
RVLEGIKLEGSHFRSDIALPAVEGRISI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=42.6229508196721, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=42.6229508196721, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=42.6229508196721, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=42.6229508196721, Blast_Score=320, Evalue=1e-87,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=42.5581395348837, Blast_Score=317, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=37.3239436619718, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=39.6396396396396, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI62471619, Length=436, Percent_Identity=40.3669724770642, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24582400, Length=436, Percent_Identity=40.3669724770642, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44683; Mature: 44683

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIGSGAREHALVLGLSKDPSVSEIHVAPGNAGMEQIATVHADATSVDDPAQMLQVA
CEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHEEECCCCCCCCHHHHHHHH
QDVQADLVVVGPEIPLVAGVADVLRQNGFAVFGPNKDAARIEGSKAFAKDVMEKAGVRTA
HHCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCEECCHHHHHHHHHHHCCCCHH
RAEQILPEASDEQRDAALDRFGPHYVVKDDGLAGGKGVVVTDDREAARAHVDAVLAAGNP
HHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHEECCCH
VLLESFLDGPEVSLFCLVDGETVVPLIPAQDHKRAYDNDEGPNTGGMGAYTPLPWLPEDG
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHH
VQRIVDEVCVPVAREMVRRGCSYSGLLYAGLAWGAEGPSVVEFNCRFGDPETQAVLALLR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHH
TPLAQVLQSVAEGTLAQAPALEWQDGYALTVVLAAEGYPESPRKGGVITGADVADASGTT
HHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCE
AVLHAGTARDEQGNVVANGGRVLNVVGTGATLADARDNAYRVLEGIKLEGSHFRSDIALP
EEEECCCCCCCCCCEEECCCEEEEEEECCCEEECCCCCHHHHHHCEEECCCCCCCCCCCE
AVEGRISI
ECCCEECC
>Mature Secondary Structure
MRILVIGSGAREHALVLGLSKDPSVSEIHVAPGNAGMEQIATVHADATSVDDPAQMLQVA
CEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHEEECCCCCCCCHHHHHHHH
QDVQADLVVVGPEIPLVAGVADVLRQNGFAVFGPNKDAARIEGSKAFAKDVMEKAGVRTA
HHCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCEECCHHHHHHHHHHHCCCCHH
RAEQILPEASDEQRDAALDRFGPHYVVKDDGLAGGKGVVVTDDREAARAHVDAVLAAGNP
HHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHEECCCH
VLLESFLDGPEVSLFCLVDGETVVPLIPAQDHKRAYDNDEGPNTGGMGAYTPLPWLPEDG
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHH
VQRIVDEVCVPVAREMVRRGCSYSGLLYAGLAWGAEGPSVVEFNCRFGDPETQAVLALLR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHH
TPLAQVLQSVAEGTLAQAPALEWQDGYALTVVLAAEGYPESPRKGGVITGADVADASGTT
HHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCE
AVLHAGTARDEQGNVVANGGRVLNVVGTGATLADARDNAYRVLEGIKLEGSHFRSDIALP
EEEECCCCCCCCCCEEECCCEEEEEEECCCEEECCCCCHHHHHHCEEECCCCCCCCCCCE
AVEGRISI
ECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]