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The map label for this gene is purF [H]

Identifier: 38234487

GI number: 38234487

Start: 1972042

End: 1973523

Strand: Reverse

Name: purF [H]

Synonym: DIP1920

Alternate gene names: 38234487

Gene position: 1973523-1972042 (Counterclockwise)

Preceding gene: 38234488

Following gene: 38234486

Centisome position: 79.3

GC content: 57.56

Gene sequence:

>1482_bases
TTGACAGACCTTGACGACCGCGGCGAACAGTCTCCGCGCGAGGAATGTGGCGTTTTTGGTGTGTGGGCTCCAGGCGAAGA
AGTATCCAAGCTCACCTATTTTGGACTTTTCGCCCTGCAGCACCGTGGACAAGAAGCTGCGGGAATCGCCGTAGGTGACG
GCGATCAAGTCGTGGTATTTAAAGACTTGGGGCTTGTGTCCCAGGTGTTTGACGAACCTACCTTGGAATCCCTCAGCGGA
GACGTGGCCATCGGCCACACCCGCTATTCCACCGCAGGTGGAGTGATGTGGGAAAACTCCCAACCAATGTTCCGTGTAAC
CCCAGAAGGAACCGATGTGGCGCTGTGCCACAACGGCAATCTGGTGAACTACCTCGAGCTCATGGCAGAAGCCGCCGAGC
ACGATTTGGTAAAACCTGACACTGAGCCCTCCGATTCCGATGTGATGACAGCCCTGCTGGCACACGGAGTATCGGAGGGT
ACATCTTTGCTTGATTCCGCCCGAGCACTCCTGCCGCGTATGCGTGGTGCGTTCTGCTTGACCTTTACCGATGGTCACAC
CATGTATGCTGCTCGTGACCCATGGGGCGTTCGCCCTCTCGTACTGGGTCGTCTCGAGCGCGGCTGGGTGGTTGCCTCAG
AAACGTGTGCACTCGACATCGTGGGTGCGTCGTTTGTTCGCGAGATCGAGCCAGGTGAGCTCGTTGCTATCGACGGCGCC
GGCATCCGTAGCGAACGTTTTGCACAACCGCAACGCAAAACCTGTGTTTTTGAGTATGTGTACCTTGCTCGCCCAGACTC
CACAATCCACGGCCGCAACGTCAACGCAACCCGTATGGAGATCGGACGAAGGCTAGCACAGGAATCGCCTATCGACGGCG
ACCTTGTCATCCCAGTCCCAGAATCCGGCAACCCCGCTGCGGTGGGTTACGCCCAAGAATCGAAGATTCCTTTCGGTCAA
GGATTGGTGAAAAACGCCTACGTTGGCCGCACCTTTATTCAGCCGTCGCAAACCCTGCGCCAGCTGGGTATTCGCCTCAA
GCTCAACCCGCTGCGTGAGGTAATCCAAGGAAAGCGCCTGATCGTTGTAGATGATTCCATTGTGCGTGGCAACACCCAGC
GAGCCCTGATCCGCATGCTGCGTGAAGCCGGTGCCGCCGAGGTTCACGTGCGTATCGCCTCGCCACCTGTGAAGTGGCCA
TGCTTCTACGGCATCGACTTTGCAAGCCCCGGCGAACTGATCGCCAACGCTGTCACCGGCGATAACGAAGCCGAGGTTGT
ACAGTCAGTCTGTACCGCCCTTGGCGCAGATTCTTTGGGATACGCCTCTACCGACGCCATGATCGAAGCCACTCAGCAGT
CCCGCGCCGAACTATGTTGTGCATGTTTCGACGGCGAATACCCCATCGGTATGCCTGCCGGAAACAAGAACGCAGAGCTT
GTTTCTGCGATGAAACGCAGCTCGTGCGCAAGCCTCGACTAA

Upstream 100 bases:

>100_bases
GTGCAACGTGGCAATGCACGTTACTATGGGCAACGTGGCACAAACTGACCAGCCCCAAAAAGACAATCTTCCGTTTGCTT
CCACTACCGTACCCTCAACA

Downstream 100 bases:

>100_bases
CGGCGAATCACCACCTATACCTACACCCCAAACCAACATAAGGATTACAGGTCCCCCACATGACGGAAAAGAACGACAAC
GTTTCCTACGCTGCCGCAGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTDLDDRGEQSPREECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGDQVVVFKDLGLVSQVFDEPTLESLSG
DVAIGHTRYSTAGGVMWENSQPMFRVTPEGTDVALCHNGNLVNYLELMAEAAEHDLVKPDTEPSDSDVMTALLAHGVSEG
TSLLDSARALLPRMRGAFCLTFTDGHTMYAARDPWGVRPLVLGRLERGWVVASETCALDIVGASFVREIEPGELVAIDGA
GIRSERFAQPQRKTCVFEYVYLARPDSTIHGRNVNATRMEIGRRLAQESPIDGDLVIPVPESGNPAAVGYAQESKIPFGQ
GLVKNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIQGKRLIVVDDSIVRGNTQRALIRMLREAGAAEVHVRIASPPVKWP
CFYGIDFASPGELIANAVTGDNEAEVVQSVCTALGADSLGYASTDAMIEATQQSRAELCCACFDGEYPIGMPAGNKNAEL
VSAMKRSSCASLD

Sequences:

>Translated_493_residues
MTDLDDRGEQSPREECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGDQVVVFKDLGLVSQVFDEPTLESLSG
DVAIGHTRYSTAGGVMWENSQPMFRVTPEGTDVALCHNGNLVNYLELMAEAAEHDLVKPDTEPSDSDVMTALLAHGVSEG
TSLLDSARALLPRMRGAFCLTFTDGHTMYAARDPWGVRPLVLGRLERGWVVASETCALDIVGASFVREIEPGELVAIDGA
GIRSERFAQPQRKTCVFEYVYLARPDSTIHGRNVNATRMEIGRRLAQESPIDGDLVIPVPESGNPAAVGYAQESKIPFGQ
GLVKNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIQGKRLIVVDDSIVRGNTQRALIRMLREAGAAEVHVRIASPPVKWP
CFYGIDFASPGELIANAVTGDNEAEVVQSVCTALGADSLGYASTDAMIEATQQSRAELCCACFDGEYPIGMPAGNKNAEL
VSAMKRSSCASLD
>Mature_492_residues
TDLDDRGEQSPREECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGDQVVVFKDLGLVSQVFDEPTLESLSGD
VAIGHTRYSTAGGVMWENSQPMFRVTPEGTDVALCHNGNLVNYLELMAEAAEHDLVKPDTEPSDSDVMTALLAHGVSEGT
SLLDSARALLPRMRGAFCLTFTDGHTMYAARDPWGVRPLVLGRLERGWVVASETCALDIVGASFVREIEPGELVAIDGAG
IRSERFAQPQRKTCVFEYVYLARPDSTIHGRNVNATRMEIGRRLAQESPIDGDLVIPVPESGNPAAVGYAQESKIPFGQG
LVKNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIQGKRLIVVDDSIVRGNTQRALIRMLREAGAAEVHVRIASPPVKWPC
FYGIDFASPGELIANAVTGDNEAEVVQSVCTALGADSLGYASTDAMIEATQQSRAELCCACFDGEYPIGMPAGNKNAELV
SAMKRSSCASLD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=39.2649903288201, Blast_Score=345, Evalue=8e-95,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=37.6050420168067, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1790167, Length=226, Percent_Identity=26.9911504424779, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=488, Percent_Identity=38.9344262295082, Blast_Score=309, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6323958, Length=486, Percent_Identity=32.5102880658436, Blast_Score=243, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24659598, Length=496, Percent_Identity=42.3387096774194, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24659604, Length=472, Percent_Identity=43.0084745762712, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=41.8, Blast_Score=352, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53162; Mature: 53031

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLDDRGEQSPREECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGDQVVVF
CCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEE
KDLGLVSQVFDEPTLESLSGDVAIGHTRYSTAGGVMWENSQPMFRVTPEGTDVALCHNGN
ECCCHHHHHHCCCCHHHCCCCEEECCEEECCCCCEEECCCCCCEEECCCCCEEEEEECCC
LVNYLELMAEAAEHDLVKPDTEPSDSDVMTALLAHGVSEGTSLLDSARALLPRMRGAFCL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
TFTDGHTMYAARDPWGVRPLVLGRLERGWVVASETCALDIVGASFVREIEPGELVAIDGA
EEECCCEEEEECCCCCCCHHHHHCCCCCEEEEECCEEEEEECHHHHHCCCCCCEEEECCC
GIRSERFAQPQRKTCVFEYVYLARPDSTIHGRNVNATRMEIGRRLAQESPIDGDLVIPVP
CCCHHHHCCCHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECC
ESGNPAAVGYAQESKIPFGQGLVKNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIQGKRL
CCCCCCEEEECCCCCCCCCCCHHHHHHCCCEECCHHHHHHHCCCEEEEHHHHHHHCCCEE
IVVDDSIVRGNTQRALIRMLREAGAAEVHVRIASPPVKWPCFYGIDFASPGELIANAVTG
EEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCC
DNEAEVVQSVCTALGADSLGYASTDAMIEATQQSRAELCCACFDGEYPIGMPAGNKNAEL
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHH
VSAMKRSSCASLD
HHHHHHHCCCCCC
>Mature Secondary Structure 
TDLDDRGEQSPREECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGDQVVVF
CCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEE
KDLGLVSQVFDEPTLESLSGDVAIGHTRYSTAGGVMWENSQPMFRVTPEGTDVALCHNGN
ECCCHHHHHHCCCCHHHCCCCEEECCEEECCCCCEEECCCCCCEEECCCCCEEEEEECCC
LVNYLELMAEAAEHDLVKPDTEPSDSDVMTALLAHGVSEGTSLLDSARALLPRMRGAFCL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
TFTDGHTMYAARDPWGVRPLVLGRLERGWVVASETCALDIVGASFVREIEPGELVAIDGA
EEECCCEEEEECCCCCCCHHHHHCCCCCEEEEECCEEEEEECHHHHHCCCCCCEEEECCC
GIRSERFAQPQRKTCVFEYVYLARPDSTIHGRNVNATRMEIGRRLAQESPIDGDLVIPVP
CCCHHHHCCCHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECC
ESGNPAAVGYAQESKIPFGQGLVKNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIQGKRL
CCCCCCEEEECCCCCCCCCCCHHHHHHCCCEECCHHHHHHHCCCEEEEHHHHHHHCCCEE
IVVDDSIVRGNTQRALIRMLREAGAAEVHVRIASPPVKWPCFYGIDFASPGELIANAVTG
EEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCC
DNEAEVVQSVCTALGADSLGYASTDAMIEATQQSRAELCCACFDGEYPIGMPAGNKNAEL
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHH
VSAMKRSSCASLD
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]