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The map label for this gene is nrdE [H]

Identifier: 38234437

GI number: 38234437

Start: 1911340

End: 1913499

Strand: Reverse

Name: nrdE [H]

Synonym: DIP1867

Alternate gene names: 38234437

Gene position: 1913499-1911340 (Counterclockwise)

Preceding gene: 38234438

Following gene: 38234435

Centisome position: 76.89

GC content: 52.64

Gene sequence:

>2160_bases
GTGAATCAATCCATGGGAAGGCACGTCGCTGAGCCAGTTTCCCGCGCTGAGCAACTCGACTACCACGCACTGAATGCACT
GTTGAACCTTTATGACGCAAACGGGCGAATCCAATTTGATAAAGACCGCGAAGCGGCCAACCAGTTCTTCCTGCAGCACG
TCAATCAAAACACAGTGTTCTTCCACGACCTAGAAGAAAAGATCGAATACCTCGTAGAAAACAACTACTACGAGCCAGAA
GTGCTGGACAAGTACTCTTTTGCAGGCATCAAAGACCTGTTCAAGCAGGCCTACGCGCATAAGTTCCGCTTCAAGTCATT
CTTGGGTGCCTACAAGTACTACACCTCGTACACGCTCAAAACCTTCGACGGTCGACGCTACCTCGAGCGCTTCGAAGATC
GCGTGTGCATGGTGGCACTAACGCTTGCCGACGGATCCATCGAGCTGGCTCGTAACCTCGTTGACGAGATCATGACCGGA
CGTTTCCAGCCGGCAACCCCGACCTTCCTTAACTCCGGTAAGGCACAGCGCGGCGAACCAGTATCGTGCTTCTTGTTGCG
TATCGAAGACAACATGGAGTCGATCGGACGTTCCATTAACTCCGCATTGCAGCTGTCCAAGCGTGGTGGTGGCGTGGCGT
TGCTGCTGAGCAACCTGCGTGAATCCGGTGCACCGATCAAGAAGATTGAGAACCAGTCTTCCGGTGTTATCCCTGTGATG
AAGCTGCTCGAGGATTCCTTCTCCTACGCTAACCAGCTGGGCGCACGTCAAGGTGCAGGTGCGGTGTACCTGAATGCTCA
CCACCCAGACATCTTGAACTTCCTGGACACCAAGCGCGAAAATGCCGATGAGAAGATCCGCATTAAGACATTGTCGCTGG
GCATTGTGATCCCAGACATCACCTTTGAGCTGGCTAAGCGAAACGAAGACATGTATCTGTTTAGCCCCTACGACGTCGAG
CGCGTCTACGGTAAGCCATTTGCGGATATCTCTGTCACCGAACTCTATGCCGACATGGTCGAAGATCCGCGTATCCGCAA
GACCAAGATCAACGCCCGTTCGTTCTTCCAAACGATCGCCGAGATCCAGTTCGAGTCCGGTTACCCGTACATCATGTTCG
AAGATACCGTTAACCGCGCCAACCCCATCGAAGGCCGGATTAACATGTCTAACCTGTGCTCGGAGATCCTGCAGGTCAAC
TCGCCATCAGTTCTTAATGCTGATCTGACCTACGAGCATGTCGGCGAAGACATCTCGTGCAACCTTGGCTCCATGAACAT
CGCTATGGCCATGGATTCCACTGACTTTGCTAAGACCGTGGAAACCGCGATTCGTGGTTTGACCGCGGTGTCCGAGCAGA
CCTCCATCGATTCCGTGCCATCCATTAAGAAGGGCAACGATGCAGCTCATGCCATCGGCCTTGGCCAGATGAACCTGCAC
GGCTACTTGGGTCGTGAGCATATCTTCTACGGATCAGAAGAAGGCTTGGACTTTACCAACGCCTACTTTGCCGCCGTGTT
GTATCAATGCCTGCGCGCTTCTCACAAGATTGCTGCGGAACGCGGCCAGCGTTTTGAAGGCTTTGAAACTTCCGACTATG
CCGATGGCAGCTACTTTGATCGTTTCGATCCAGCTGACTTTGCGCCGAAGACCGCAAAGGTTCAAGAGCTATTTGAAAAC
TCCACCATTGTTGTGCCTACTGCAGAGGACTGGGAAGAGCTCAAGCAGTTGGTCATGAAGGACGGCTTGTTTAACCGCTA
CCTTCAGGCTGTGCCTCCGACGGGTTCGATCTCGTACATCAACAACTCCACCTCGTCGATTCACCCGATCGCCTCTCGTA
TTGAGATCCGTAAGGAAGGCAAGATCGGTCGTGTGTACTACCCAGCGCCGCACATGGATAACGACAACTTGGAGTACTAC
CAAGATGCCTATGAGATTGGCTTTGAAAAGGTTATTGATACCTATGCGGTAGCCACCAAGTATGTTGACCAGGGACTTTC
GTTGACGCTGTTCTTCAAGGACAACGTGACAACCCGTGACATCAACCGCGCGCAGATCTACGCATGGCGTAAAGGAATTA
AGTCCCTGTACTATATTCGCCTCCGCCAAGTTGCCCTTGAGGGTACCGAGGTCGAGGGTTGCGTATCCTGCATGCTATAA

Upstream 100 bases:

>100_bases
GTGCGCGACGGTCTGCGAGAAAACGCAGCAGCACTAGGCCTTGAAAAATAACTGAATAATTTTCCCCTATCCATCAAGCA
ATCTTGTAAGGATTTGTCTC

Downstream 100 bases:

>100_bases
AAAAATCCCCCTCGCGGGGGATTTTTCGTAGAAGCTTGCTGGGGGAGGGGATTAACGCTCTCCCAGTTCGGCATCCATGC
GAAGCAGTCCGGTGCCGGTA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MNQSMGRHVAEPVSRAEQLDYHALNALLNLYDANGRIQFDKDREAANQFFLQHVNQNTVFFHDLEEKIEYLVENNYYEPE
VLDKYSFAGIKDLFKQAYAHKFRFKSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGSIELARNLVDEIMTG
RFQPATPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLRESGAPIKKIENQSSGVIPVM
KLLEDSFSYANQLGARQGAGAVYLNAHHPDILNFLDTKRENADEKIRIKTLSLGIVIPDITFELAKRNEDMYLFSPYDVE
RVYGKPFADISVTELYADMVEDPRIRKTKINARSFFQTIAEIQFESGYPYIMFEDTVNRANPIEGRINMSNLCSEILQVN
SPSVLNADLTYEHVGEDISCNLGSMNIAMAMDSTDFAKTVETAIRGLTAVSEQTSIDSVPSIKKGNDAAHAIGLGQMNLH
GYLGREHIFYGSEEGLDFTNAYFAAVLYQCLRASHKIAAERGQRFEGFETSDYADGSYFDRFDPADFAPKTAKVQELFEN
STIVVPTAEDWEELKQLVMKDGLFNRYLQAVPPTGSISYINNSTSSIHPIASRIEIRKEGKIGRVYYPAPHMDNDNLEYY
QDAYEIGFEKVIDTYAVATKYVDQGLSLTLFFKDNVTTRDINRAQIYAWRKGIKSLYYIRLRQVALEGTEVEGCVSCML

Sequences:

>Translated_719_residues
MNQSMGRHVAEPVSRAEQLDYHALNALLNLYDANGRIQFDKDREAANQFFLQHVNQNTVFFHDLEEKIEYLVENNYYEPE
VLDKYSFAGIKDLFKQAYAHKFRFKSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGSIELARNLVDEIMTG
RFQPATPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLRESGAPIKKIENQSSGVIPVM
KLLEDSFSYANQLGARQGAGAVYLNAHHPDILNFLDTKRENADEKIRIKTLSLGIVIPDITFELAKRNEDMYLFSPYDVE
RVYGKPFADISVTELYADMVEDPRIRKTKINARSFFQTIAEIQFESGYPYIMFEDTVNRANPIEGRINMSNLCSEILQVN
SPSVLNADLTYEHVGEDISCNLGSMNIAMAMDSTDFAKTVETAIRGLTAVSEQTSIDSVPSIKKGNDAAHAIGLGQMNLH
GYLGREHIFYGSEEGLDFTNAYFAAVLYQCLRASHKIAAERGQRFEGFETSDYADGSYFDRFDPADFAPKTAKVQELFEN
STIVVPTAEDWEELKQLVMKDGLFNRYLQAVPPTGSISYINNSTSSIHPIASRIEIRKEGKIGRVYYPAPHMDNDNLEYY
QDAYEIGFEKVIDTYAVATKYVDQGLSLTLFFKDNVTTRDINRAQIYAWRKGIKSLYYIRLRQVALEGTEVEGCVSCML
>Mature_719_residues
MNQSMGRHVAEPVSRAEQLDYHALNALLNLYDANGRIQFDKDREAANQFFLQHVNQNTVFFHDLEEKIEYLVENNYYEPE
VLDKYSFAGIKDLFKQAYAHKFRFKSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGSIELARNLVDEIMTG
RFQPATPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLRESGAPIKKIENQSSGVIPVM
KLLEDSFSYANQLGARQGAGAVYLNAHHPDILNFLDTKRENADEKIRIKTLSLGIVIPDITFELAKRNEDMYLFSPYDVE
RVYGKPFADISVTELYADMVEDPRIRKTKINARSFFQTIAEIQFESGYPYIMFEDTVNRANPIEGRINMSNLCSEILQVN
SPSVLNADLTYEHVGEDISCNLGSMNIAMAMDSTDFAKTVETAIRGLTAVSEQTSIDSVPSIKKGNDAAHAIGLGQMNLH
GYLGREHIFYGSEEGLDFTNAYFAAVLYQCLRASHKIAAERGQRFEGFETSDYADGSYFDRFDPADFAPKTAKVQELFEN
STIVVPTAEDWEELKQLVMKDGLFNRYLQAVPPTGSISYINNSTSSIHPIASRIEIRKEGKIGRVYYPAPHMDNDNLEYY
QDAYEIGFEKVIDTYAVATKYVDQGLSLTLFFKDNVTTRDINRAQIYAWRKGIKSLYYIRLRQVALEGTEVEGCVSCML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=706, Percent_Identity=25.7790368271955, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1789030, Length=715, Percent_Identity=70.2097902097902, Blast_Score=1049, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=586, Percent_Identity=25.7679180887372, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17555242, Length=645, Percent_Identity=26.3565891472868, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=646, Percent_Identity=26.0061919504644, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=646, Percent_Identity=25.5417956656347, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI17352481, Length=649, Percent_Identity=24.9614791987673, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81434; Mature: 81434

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSMGRHVAEPVSRAEQLDYHALNALLNLYDANGRIQFDKDREAANQFFLQHVNQNTVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCEEE
FHDLEEKIEYLVENNYYEPEVLDKYSFAGIKDLFKQAYAHKFRFKSFLGAYKYYTSYTLK
EECHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TFDGRRYLERFEDRVCMVALTLADGSIELARNLVDEIMTGRFQPATPTFLNSGKAQRGEP
ECCCHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCC
VSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLRESGAPIKKIENQSSGVIPVM
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHH
KLLEDSFSYANQLGARQGAGAVYLNAHHPDILNFLDTKRENADEKIRIKTLSLGIVIPDI
HHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCC
TFELAKRNEDMYLFSPYDVERVYGKPFADISVTELYADMVEDPRIRKTKINARSFFQTIA
HHHHHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
EIQFESGYPYIMFEDTVNRANPIEGRINMSNLCSEILQVNSPSVLNADLTYEHVGEDISC
HHEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEE
NLGSMNIAMAMDSTDFAKTVETAIRGLTAVSEQTSIDSVPSIKKGNDAAHAIGLGQMNLH
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEECCCEECC
GYLGREHIFYGSEEGLDFTNAYFAAVLYQCLRASHKIAAERGQRFEGFETSDYADGSYFD
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RFDPADFAPKTAKVQELFENSTIVVPTAEDWEELKQLVMKDGLFNRYLQAVPPTGSISYI
CCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEE
NNSTSSIHPIASRIEIRKEGKIGRVYYPAPHMDNDNLEYYQDAYEIGFEKVIDTYAVATK
CCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVDQGLSLTLFFKDNVTTRDINRAQIYAWRKGIKSLYYIRLRQVALEGTEVEGCVSCML
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MNQSMGRHVAEPVSRAEQLDYHALNALLNLYDANGRIQFDKDREAANQFFLQHVNQNTVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCEEE
FHDLEEKIEYLVENNYYEPEVLDKYSFAGIKDLFKQAYAHKFRFKSFLGAYKYYTSYTLK
EECHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TFDGRRYLERFEDRVCMVALTLADGSIELARNLVDEIMTGRFQPATPTFLNSGKAQRGEP
ECCCHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCC
VSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLRESGAPIKKIENQSSGVIPVM
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHH
KLLEDSFSYANQLGARQGAGAVYLNAHHPDILNFLDTKRENADEKIRIKTLSLGIVIPDI
HHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCC
TFELAKRNEDMYLFSPYDVERVYGKPFADISVTELYADMVEDPRIRKTKINARSFFQTIA
HHHHHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
EIQFESGYPYIMFEDTVNRANPIEGRINMSNLCSEILQVNSPSVLNADLTYEHVGEDISC
HHEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEE
NLGSMNIAMAMDSTDFAKTVETAIRGLTAVSEQTSIDSVPSIKKGNDAAHAIGLGQMNLH
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEECCCEECC
GYLGREHIFYGSEEGLDFTNAYFAAVLYQCLRASHKIAAERGQRFEGFETSDYADGSYFD
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RFDPADFAPKTAKVQELFENSTIVVPTAEDWEELKQLVMKDGLFNRYLQAVPPTGSISYI
CCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEE
NNSTSSIHPIASRIEIRKEGKIGRVYYPAPHMDNDNLEYYQDAYEIGFEKVIDTYAVATK
CCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVDQGLSLTLFFKDNVTTRDINRAQIYAWRKGIKSLYYIRLRQVALEGTEVEGCVSCML
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]