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The map label for this gene is rph

Identifier: 38234421

GI number: 38234421

Start: 1895536

End: 1896261

Strand: Reverse

Name: rph

Synonym: DIP1851

Alternate gene names: 38234421

Gene position: 1896261-1895536 (Counterclockwise)

Preceding gene: 38234422

Following gene: 38234420

Centisome position: 76.2

GC content: 57.99

Gene sequence:

>726_bases
ATGACTGATTTTTCTCGTGCAGACGGCCGCGCCGTCGACCAGATGCGTACTGTTAAAATCACCCGCGGTTTTACCTCAAA
CCCAGCCGGCAGCGTTCTCGTTGAATTCGGCAACACTCGCGTGATGTGTACCGCCTCCGTTGAACTCGGTGTTCCTCGCT
TCAAGCGTGACTCCGGCGAAGGTTGGCTCACCGCCGAATACGCCATGCTGCCTGCTGCAACCGCTGAGCGCAATGCTCGC
GAGTCCATGCGCGGCAAGGTGAAGGGCCGCACCCATGAGATTTCTCGTCTGATTGGGCGCTCTCTGCGTGCCGCTGTTGA
CCTTGACGAGCTTGGTGAAAACACCATCAACATCGACTGCGACGTTTTGCAGGCCGACGGTGGCACCCGCACCGCTTCGA
TCACCGGCGCATACGTGGCCCTTGCCGACGCCATCGCTGTGCTCAAAGAACAAGGCGTAGTCCCCGGTAATCCTCTGAAA
GCAGCAGTAGCTGCGGTGTCTGTCGGCGTGATCGATGGTCAAGTGTGCCTCGACTTGCCCTATGAGGAAGATTCCCGCGC
AGATGTGGACATGAACGTGATCATGCAGGGCGATCGCTTTGTGGAAATCCAGGGCACCGGTGAGCACAACACCTTTGATC
GTGATGAGCTTGCCGTCATCTTGGACTTCGCGCAGAAGGGCTGCCAAGAACTGTTCGCCGCACAGAAGGCAGCTCTAGAA
CAGTGA

Upstream 100 bases:

>100_bases
AGTGATACCAATGAGGCGCTTGCTGCGGCGCGGGCAGAATTTGACGGCGAGGTTCTCGTCGGCGAGCACGGTCTGGAGCT
GACAGTGTAGCGTAAACCGC

Downstream 100 bases:

>100_bases
AAGTACTCGTAGCCTCCAACAATGCCAAGAAGCTCGGTGAGCTACGCACCATCTTGGAAAACGCAGGTCTGAGCAGCGTC
GAGGTAGTGCCGCTTTCGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTDFSRADGRAVDQMRTVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAERNAR
ESMRGKVKGRTHEISRLIGRSLRAAVDLDELGENTINIDCDVLQADGGTRTASITGAYVALADAIAVLKEQGVVPGNPLK
AAVAAVSVGVIDGQVCLDLPYEEDSRADVDMNVIMQGDRFVEIQGTGEHNTFDRDELAVILDFAQKGCQELFAAQKAALE
Q

Sequences:

>Translated_241_residues
MTDFSRADGRAVDQMRTVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAERNAR
ESMRGKVKGRTHEISRLIGRSLRAAVDLDELGENTINIDCDVLQADGGTRTASITGAYVALADAIAVLKEQGVVPGNPLK
AAVAAVSVGVIDGQVCLDLPYEEDSRADVDMNVIMQGDRFVEIQGTGEHNTFDRDELAVILDFAQKGCQELFAAQKAALE
Q
>Mature_240_residues
TDFSRADGRAVDQMRTVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAERNARE
SMRGKVKGRTHEISRLIGRSLRAAVDLDELGENTINIDCDVLQADGGTRTASITGAYVALADAIAVLKEQGVVPGNPLKA
AVAAVSVGVIDGQVCLDLPYEEDSRADVDMNVIMQGDRFVEIQGTGEHNTFDRDELAVILDFAQKGCQELFAAQKAALEQ

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=208, Percent_Identity=60.5769230769231, Blast_Score=251, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71981632, Length=240, Percent_Identity=27.9166666666667, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_CORDI (Q6NFN6)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940188.1
- ProteinModelPortal:   Q6NFN6
- SMR:   Q6NFN6
- GeneID:   2649226
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1851
- NMPDR:   fig|257309.1.peg.1779
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q6NFN6
- ProtClustDB:   PRK00173
- BioCyc:   CDIP257309:DIP1851-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25896; Mature: 25764

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSRADGRAVDQMRTVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGE
CCCCCCCCCCHHHHEEEEEEECCCCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCC
GWLTAEYAMLPAATAERNARESMRGKVKGRTHEISRLIGRSLRAAVDLDELGENTINIDC
CEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEEEEE
DVLQADGGTRTASITGAYVALADAIAVLKEQGVVPGNPLKAAVAAVSVGVIDGQVCLDLP
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCEEEEECC
YEEDSRADVDMNVIMQGDRFVEIQGTGEHNTFDRDELAVILDFAQKGCQELFAAQKAALE
CCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
Q
C
>Mature Secondary Structure 
TDFSRADGRAVDQMRTVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGE
CCCCCCCCCHHHHEEEEEEECCCCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCC
GWLTAEYAMLPAATAERNARESMRGKVKGRTHEISRLIGRSLRAAVDLDELGENTINIDC
CEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEEEEE
DVLQADGGTRTASITGAYVALADAIAVLKEQGVVPGNPLKAAVAAVSVGVIDGQVCLDLP
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCEEEEECC
YEEDSRADVDMNVIMQGDRFVEIQGTGEHNTFDRDELAVILDFAQKGCQELFAAQKAALE
CCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910