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The map label for this gene is sigA [H]

Identifier: 38233991

GI number: 38233991

Start: 1423091

End: 1424599

Strand: Direct

Name: sigA [H]

Synonym: DIP1406

Alternate gene names: 38233991

Gene position: 1423091-1424599 (Clockwise)

Preceding gene: 38233990

Following gene: 38233995

Centisome position: 57.18

GC content: 49.24

Gene sequence:

>1509_bases
TTGGCGAATGCTGGCGAGGCATCCTCTGCCACTGTAAAAAAGGCAGCCAAGAAGACCGCTGCCAAGAAAACCGCACGCAA
GGTTGCCCGCAAAGTTACTGCAGTTAAAGTAGCTGCAACACCAACAGTAACAAGCTCTGCTGAATCAGCTACAGCCGAGC
CAGCAATTGAGTTGCCTTCATCTGAGCCAGTCAAAAAAGCTGCTAAAAAAACGGCCAAGAAAACGGCTAAGAAGGTCGCT
AAAAAGGCAGCAAAGAAAACTGCCAAGAAAACGACAGCAAAGAAGAGCACTCGCAAATCCGCTAAAAAAGTTGCTGCTCC
AGTTCCTGAAGAAGCGACCCAATCCAGCGAAGAGTCCGAGGACGAGCTCGAATCGCTAGCTGGCGAAGATTCAGATGAAG
ATTATGATCCGCATCTGGATGAATTTGACGACGACGATGAGATGCACACCGACGAGCTCGGAGAAGATGACTCTTCCGAT
GATGATTCAGATGAGGATGAGGGTTCTTCAGTCTGGGATGAGGACGAGTCGGCGGCATTACGTCAGGCACGTAAAGATGC
GGAGCTTTCTGCGTCTGCAGACTCCGTTCGCGCATATTTGAAACAAATCGGCAAGGTAGCCTTGCTGAATGCCGAGCAAG
AAGTTTCTTTGGCTAAGCGTATCGAGGCAGGCCTCTATGCCACTTACCGCATGGAGCAAATGGAAGAAGCGTTTAATAAC
GGGGATAAGGAAGCAAAACTTACTCCAGCTGTCAAACGCGACCTTCGTGCAATTGCAAGAGACGGACGCAAAGCCAAAAA
TCATCTACTAGAAGCAAACCTTCGTCTGGTTGTGTCACTCGCTAAGCGCTATACGGGCCGTGGCATGGCGTTCTTGGATT
TGATTCAAGAGGGCAACTTGGGTCTTATTCGTGCTGTCGAAAAGTTTGATTACACCAAGGGCTACAAGTTCTCCACATAC
GCTACTTGGTGGATTCGCCAAGCAATCACGCGTGCGATGGCAGATCAGGCCCGCACCATCCGTATCCCGGTACACATGGT
CGAAGTCATTAATAAGCTCGGACGGATTCAACGTGAGCTTCTTCAAGATCTCGGTCGCGAGCCTACCCCTCAAGAACTCG
CTAAAGAGATGGATATCACTGAAGAAAAAGTCCTTGAGATTCAGCAGTACGCTCGCGAGCCTATTTCTTTGGATCAAACC
ATCGGTGACGAGGGTGATAGTCAGCTCGGAGACTTTATTGAAGATTCTGAAGCTGTTATTGCAGTTGATGCCGTTTCGTT
CACGTTGTTGCAGGATCAACTTCAAGATGTTCTCCATACGCTCTCCGAACGCGAAGCTGGCGTTGTCAAACTCCGCTTCG
GACTTACAGATGGAATGCCAAGGACTTTAGACGAGATTGGCCAGGTCTATGGCGTTACTCGTGAACGTATTCGTCAGATT
GAGTCCAAGACGATGTCAAAGCTGCGTCACCCATCTCGTTCACAGGTACTCCGAGATTATCTCGATTAA

Upstream 100 bases:

>100_bases
GTTTTAACTTCCAATCGAAAGGGCTTACGTGGCAGCCACTGAGAATTCAGAATCTTCCGCAACGGCAACCGCTGAAGGCA
CAGCGTCTTCAGGACAAGGC

Downstream 100 bases:

>100_bases
CACCACACTTGTTCGTTGACGGTGCTCAACGAAACTGCGGAAGCAAAACTTCTAAAAAGATAATGACCTCTTTACATTTG
AAGCTATTGTAGGGGCTTGG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MANAGEASSATVKKAAKKTAAKKTARKVARKVTAVKVAATPTVTSSAESATAEPAIELPSSEPVKKAAKKTAKKTAKKVA
KKAAKKTAKKTTAKKSTRKSAKKVAAPVPEEATQSSEESEDELESLAGEDSDEDYDPHLDEFDDDDEMHTDELGEDDSSD
DDSDEDEGSSVWDEDESAALRQARKDAELSASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATYRMEQMEEAFNN
GDKEAKLTPAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTY
ATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPQELAKEMDITEEKVLEIQQYAREPISLDQT
IGDEGDSQLGDFIEDSEAVIAVDAVSFTLLQDQLQDVLHTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQI
ESKTMSKLRHPSRSQVLRDYLD

Sequences:

>Translated_502_residues
MANAGEASSATVKKAAKKTAAKKTARKVARKVTAVKVAATPTVTSSAESATAEPAIELPSSEPVKKAAKKTAKKTAKKVA
KKAAKKTAKKTTAKKSTRKSAKKVAAPVPEEATQSSEESEDELESLAGEDSDEDYDPHLDEFDDDDEMHTDELGEDDSSD
DDSDEDEGSSVWDEDESAALRQARKDAELSASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATYRMEQMEEAFNN
GDKEAKLTPAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTY
ATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPQELAKEMDITEEKVLEIQQYAREPISLDQT
IGDEGDSQLGDFIEDSEAVIAVDAVSFTLLQDQLQDVLHTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQI
ESKTMSKLRHPSRSQVLRDYLD
>Mature_501_residues
ANAGEASSATVKKAAKKTAAKKTARKVARKVTAVKVAATPTVTSSAESATAEPAIELPSSEPVKKAAKKTAKKTAKKVAK
KAAKKTAKKTTAKKSTRKSAKKVAAPVPEEATQSSEESEDELESLAGEDSDEDYDPHLDEFDDDDEMHTDELGEDDSSDD
DSDEDEGSSVWDEDESAALRQARKDAELSASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATYRMEQMEEAFNNG
DKEAKLTPAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYA
TWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPQELAKEMDITEEKVLEIQQYAREPISLDQTI
GDEGDSQLGDFIEDSEAVIAVDAVSFTLLQDQLQDVLHTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIE
SKTMSKLRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=59.7510373443983, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1789098, Length=361, Percent_Identity=34.0720221606648, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 55563; Mature: 55432

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAGEASSATVKKAAKKTAAKKTARKVARKVTAVKVAATPTVTSSAESATAEPAIELPS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCC
SEPVKKAAKKTAKKTAKKVAKKAAKKTAKKTTAKKSTRKSAKKVAAPVPEEATQSSEESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHH
DELESLAGEDSDEDYDPHLDEFDDDDEMHTDELGEDDSSDDDSDEDEGSSVWDEDESAAL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RQARKDAELSASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATYRMEQMEEAFNN
HHHHHHHHHHCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDKEAKLTPAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNL
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
GLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQREL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHH
LQDLGREPTPQELAKEMDITEEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVI
HHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEE
AVDAVSFTLLQDQLQDVLHTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQI
EEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
ANAGEASSATVKKAAKKTAAKKTARKVARKVTAVKVAATPTVTSSAESATAEPAIELPS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCC
SEPVKKAAKKTAKKTAKKVAKKAAKKTAKKTTAKKSTRKSAKKVAAPVPEEATQSSEESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHH
DELESLAGEDSDEDYDPHLDEFDDDDEMHTDELGEDDSSDDDSDEDEGSSVWDEDESAAL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RQARKDAELSASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATYRMEQMEEAFNN
HHHHHHHHHHCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDKEAKLTPAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNL
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
GLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQREL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHH
LQDLGREPTPQELAKEMDITEEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVI
HHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEE
AVDAVSFTLLQDQLQDVLHTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQI
EEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]