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The map label for this gene is carA

Identifier: 38233918

GI number: 38233918

Start: 1346154

End: 1347293

Strand: Reverse

Name: carA

Synonym: DIP1332

Alternate gene names: 38233918

Gene position: 1347293-1346154 (Counterclockwise)

Preceding gene: 38233919

Following gene: 38233917

Centisome position: 54.14

GC content: 47.98

Gene sequence:

>1140_bases
GTGACGTCCCAAGATCGAAGTGAAGCCGTACTTGTGTTGGCAGATGGCCGCATATTTAAAGGAGAGTCTTTCGGCGCGGA
AGGAGCGACTCTAGGCGAAGCAGTTTTTACTACGGCAATGAGTGGTTATCAAGAGACCATGACAGATCCGTCGTATCACC
GTCAGATTGTGGTAGCTACGGCTCCGCAGATCGGTAACACCGGCTGGAATGATGAAGACGGTGAATCCCATGGTGACAAG
ATTTGGGTTGCTGGTCTTGTGATTCGGGATTTGTCTACGGCTGTATCTAACTGGCGCGCAAAGCGCAGCTTGGAAGATGA
AATGCGCGAACAGAACATTGTCGGAATTTCTGGTGTGGATACACGTGCAATAGTCCGGCATTTACGTAATTTCGGTTCAG
TCGCTGCAGGTATTTTCTCAGGAGAGGCGGCGAAGCAGCCTCATGATGAGCTCATTGAGATAGTAAAATCACAGCCTTCA
ATGGCAGGAGCAGACTTAGCTAGCGAGGTATCTACCCAAGAAGCTTATGTCGTAGAACCAGACGGTGAAGCACTTTATAC
CGTTGTTGCTTATGACATGGGTATTAAGACGAACACTCCTCGTAATTTCTCCCGTCGTGGAATTAGAACGGTAGTCGTTC
CTGCAAATACTCCTGCAGAGGACATCGCTCAATATAACCCCGACGGTGTTTTTGTATCCAATGGTCCTGGCGATCCAGCT
ACTGCCGATGTGATGGTAGGGATCGTGCAAGAAATTCTGGCACAGAAAATTCCATTTTTCGGAATTTGCTTTGGCAACCA
GATTCTTGGTCGTGCACTAGGAATGAACACCTACAAAATGAAATTTGGCCACCGTGGTATCAACGTTCCTGTTTTGAATC
ATCTCACTGGGAAAATTGATATTACAGCGCAAAACCATGGATTTGCACTAGAAGGCACAGCGCTTGAGCAATTTGACACG
CCGTTCGGCCCTGCCCATGTAACGCATACTTGCCTTAACGACGACACTGTGGAAGGTGTAGCGCTGGTGAATGGTTTGGC
TTACTCAGTGCAGTATCACCCTGAAGCCGCAGCGGGCCCGCATGATGCAAATCCTTTGTTTGACCAGTTTGTCGAGCTTA
TTCAAGCAAATAAGAAATAA

Upstream 100 bases:

>100_bases
CAGCTGGCTTTATCGCCTGAGCACCTCAAAACGCACAAAGTGAAACGCGGATATTGCGTTGATATCCGTGTACCCAAAAA
CAAAAGTGAAAGGCGACAGA

Downstream 100 bases:

>100_bases
TCACCAGGAAGGTAAAAATAATGCCAAAGCGCAATGACATCAAACACGTCCTGGTTATCGGTTCCGGTCCAATCGTTATC
GGACAAGCGTGTGAGTTTGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTSQDRSEAVLVLADGRIFKGESFGAEGATLGEAVFTTAMSGYQETMTDPSYHRQIVVATAPQIGNTGWNDEDGESHGDK
IWVAGLVIRDLSTAVSNWRAKRSLEDEMREQNIVGISGVDTRAIVRHLRNFGSVAAGIFSGEAAKQPHDELIEIVKSQPS
MAGADLASEVSTQEAYVVEPDGEALYTVVAYDMGIKTNTPRNFSRRGIRTVVVPANTPAEDIAQYNPDGVFVSNGPGDPA
TADVMVGIVQEILAQKIPFFGICFGNQILGRALGMNTYKMKFGHRGINVPVLNHLTGKIDITAQNHGFALEGTALEQFDT
PFGPAHVTHTCLNDDTVEGVALVNGLAYSVQYHPEAAAGPHDANPLFDQFVELIQANKK

Sequences:

>Translated_379_residues
MTSQDRSEAVLVLADGRIFKGESFGAEGATLGEAVFTTAMSGYQETMTDPSYHRQIVVATAPQIGNTGWNDEDGESHGDK
IWVAGLVIRDLSTAVSNWRAKRSLEDEMREQNIVGISGVDTRAIVRHLRNFGSVAAGIFSGEAAKQPHDELIEIVKSQPS
MAGADLASEVSTQEAYVVEPDGEALYTVVAYDMGIKTNTPRNFSRRGIRTVVVPANTPAEDIAQYNPDGVFVSNGPGDPA
TADVMVGIVQEILAQKIPFFGICFGNQILGRALGMNTYKMKFGHRGINVPVLNHLTGKIDITAQNHGFALEGTALEQFDT
PFGPAHVTHTCLNDDTVEGVALVNGLAYSVQYHPEAAAGPHDANPLFDQFVELIQANKK
>Mature_378_residues
TSQDRSEAVLVLADGRIFKGESFGAEGATLGEAVFTTAMSGYQETMTDPSYHRQIVVATAPQIGNTGWNDEDGESHGDKI
WVAGLVIRDLSTAVSNWRAKRSLEDEMREQNIVGISGVDTRAIVRHLRNFGSVAAGIFSGEAAKQPHDELIEIVKSQPSM
AGADLASEVSTQEAYVVEPDGEALYTVVAYDMGIKTNTPRNFSRRGIRTVVVPANTPAEDIAQYNPDGVFVSNGPGDPAT
ADVMVGIVQEILAQKIPFFGICFGNQILGRALGMNTYKMKFGHRGINVPVLNHLTGKIDITAQNHGFALEGTALEQFDTP
FGPAHVTHTCLNDDTVEGVALVNGLAYSVQYHPEAAAGPHDANPLFDQFVELIQANKK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=376, Percent_Identity=37.5, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=37.696335078534, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=37.696335078534, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786215, Length=388, Percent_Identity=45.6185567010309, Blast_Score=317, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=36.4583333333333, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=38.1546134663342, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=384, Percent_Identity=35.15625, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=36.6925064599483, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=36.6925064599483, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_CORDI (Q6NH15)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939685.1
- ProteinModelPortal:   Q6NH15
- SMR:   Q6NH15
- GeneID:   2649886
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1332
- NMPDR:   fig|257309.1.peg.1276
- HOGENOM:   HBG286341
- OMA:   IFISETY
- PhylomeDB:   Q6NH15
- ProtClustDB:   PRK12564
- BioCyc:   CDIP257309:DIP1332-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40772; Mature: 40641

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 263-263 ACT_SITE 353-353 ACT_SITE 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQDRSEAVLVLADGRIFKGESFGAEGATLGEAVFTTAMSGYQETMTDPSYHRQIVVAT
CCCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
APQIGNTGWNDEDGESHGDKIWVAGLVIRDLSTAVSNWRAKRSLEDEMREQNIVGISGVD
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
TRAIVRHLRNFGSVAAGIFSGEAAKQPHDELIEIVKSQPSMAGADLASEVSTQEAYVVEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECC
DGEALYTVVAYDMGIKTNTPRNFSRRGIRTVVVPANTPAEDIAQYNPDGVFVSNGPGDPA
CCCEEEEEEEEECCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHCCCCEEEEECCCCCCH
TADVMVGIVQEILAQKIPFFGICFGNQILGRALGMNTYKMKFGHRGINVPVLNHLTGKID
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCEEE
ITAQNHGFALEGTALEQFDTPFGPAHVTHTCLNDDTVEGVALVNGLAYSVQYHPEAAAGP
EEECCCCEEEECCHHHHCCCCCCCCHHEEEECCCCCCCHHHHHCCEEEEEEECCCCCCCC
HDANPLFDQFVELIQANKK
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSQDRSEAVLVLADGRIFKGESFGAEGATLGEAVFTTAMSGYQETMTDPSYHRQIVVAT
CCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
APQIGNTGWNDEDGESHGDKIWVAGLVIRDLSTAVSNWRAKRSLEDEMREQNIVGISGVD
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
TRAIVRHLRNFGSVAAGIFSGEAAKQPHDELIEIVKSQPSMAGADLASEVSTQEAYVVEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECC
DGEALYTVVAYDMGIKTNTPRNFSRRGIRTVVVPANTPAEDIAQYNPDGVFVSNGPGDPA
CCCEEEEEEEEECCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHCCCCEEEEECCCCCCH
TADVMVGIVQEILAQKIPFFGICFGNQILGRALGMNTYKMKFGHRGINVPVLNHLTGKID
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCEEE
ITAQNHGFALEGTALEQFDTPFGPAHVTHTCLNDDTVEGVALVNGLAYSVQYHPEAAAGP
EEECCCCEEEECCHHHHCCCCCCCCHHEEEECCCCCCCHHHHHCCEEEEEEECCCCCCCC
HDANPLFDQFVELIQANKK
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910