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The map label for this gene is tpiA

Identifier: 38233894

GI number: 38233894

Start: 1318869

End: 1319651

Strand: Reverse

Name: tpiA

Synonym: DIP1308

Alternate gene names: 38233894

Gene position: 1319651-1318869 (Counterclockwise)

Preceding gene: 38233895

Following gene: 38233893

Centisome position: 53.03

GC content: 48.66

Gene sequence:

>783_bases
ATGGCACGTACTCCACTTATCGCAGGAAACTGGAAGATGAATCTTGATCATCTTCAAGCTGTTGCAACTGTGCAAAAACT
TGCTTTTGCACTTCCAAAGGAATACTACGAAAAAGTCGACGTAGCTGTCACCGTACCTTTTACTGATCTTCGCTCTGTTC
AAACACTTATCGAAGGCGATAAGCTCGCTATAACTTATGGTGCTCAAGATGTTTCGCAGCACGAGGCTGGTGCCTACACT
GGTGAAATCTCTGCCCAGATGCTTGCCAAACTCGGATGTACTTGGGTTGTTGTTGGGCACTCAGAGCGTCGTGAGTACCA
TGGCGAATCTTCGGAGCTTGTTGCAGTCAAAGCTAAAGCTGCGTTGAACCAAGGAATTAGCCCCATCGTTTGTGTTGGAG
AGCCCCTTGAAATTCGTGAAGCTGGAACACACGTAGAATATGTCGTCGAACAGACTCGCGCATCGTTGGCGGGTCTTACT
GCAGATGATTTGGCAAAGACTGTTATCGCCTACGAACCCGTATGGGCTATTGGTACGGGTAAAGTAGCATCCGCTGCCGA
TGCACAGGAAGTCTGCGCAGCAATTCGTAACCTCATCATTGAGCTTGCTGGTAAAGAAGTAGCTGAAGGGCTTCGCATTC
TTTATGGCGGATCAGTTAAAGCAGAAACAGTAGCCGAGATCGTAGGTCAACCGGATGTTGATGGCGGCTTAGTGGGTGGC
GCTTCTCTTGACGGTGAAGCATTCGCCAAACTAGCAGCTAATGCAGCAACTGTTGTCGACTAG

Upstream 100 bases:

>100_bases
AGGTAGGCGCGAAAATTCTTTGATACAAGAAGTATTTGCACCTACCTTTTCATTTTGGTTGTTTGCTAAAACGACCTATT
AAAAGGAAGGAAATTCCATC

Downstream 100 bases:

>100_bases
TTAGTCGCAGTAAATTAGAGTGGCTCCGTGTTGTGCCTTGCAGCCCACGGAGCCATATTGTTATAGCACTGCGACATTTG
GTGTATAGTTACTTGAGATT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MARTPLIAGNWKMNLDHLQAVATVQKLAFALPKEYYEKVDVAVTVPFTDLRSVQTLIEGDKLAITYGAQDVSQHEAGAYT
GEISAQMLAKLGCTWVVVGHSERREYHGESSELVAVKAKAALNQGISPIVCVGEPLEIREAGTHVEYVVEQTRASLAGLT
ADDLAKTVIAYEPVWAIGTGKVASAADAQEVCAAIRNLIIELAGKEVAEGLRILYGGSVKAETVAEIVGQPDVDGGLVGG
ASLDGEAFAKLAANAATVVD

Sequences:

>Translated_260_residues
MARTPLIAGNWKMNLDHLQAVATVQKLAFALPKEYYEKVDVAVTVPFTDLRSVQTLIEGDKLAITYGAQDVSQHEAGAYT
GEISAQMLAKLGCTWVVVGHSERREYHGESSELVAVKAKAALNQGISPIVCVGEPLEIREAGTHVEYVVEQTRASLAGLT
ADDLAKTVIAYEPVWAIGTGKVASAADAQEVCAAIRNLIIELAGKEVAEGLRILYGGSVKAETVAEIVGQPDVDGGLVGG
ASLDGEAFAKLAANAATVVD
>Mature_259_residues
ARTPLIAGNWKMNLDHLQAVATVQKLAFALPKEYYEKVDVAVTVPFTDLRSVQTLIEGDKLAITYGAQDVSQHEAGAYTG
EISAQMLAKLGCTWVVVGHSERREYHGESSELVAVKAKAALNQGISPIVCVGEPLEIREAGTHVEYVVEQTRASLAGLTA
DDLAKTVIAYEPVWAIGTGKVASAADAQEVCAAIRNLIIELAGKEVAEGLRILYGGSVKAETVAEIVGQPDVDGGLVGGA
SLDGEAFAKLAANAATVVD

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=42.8571428571429, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI226529917, Length=245, Percent_Identity=42.8571428571429, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=43.5483870967742, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=42.8571428571429, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=41.2955465587045, Blast_Score=172, Evalue=5e-44,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=41.869918699187, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=41.869918699187, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=41.869918699187, Blast_Score=171, Evalue=6e-43,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CORDI (Q6NH37)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939661.1
- HSSP:   P36204
- ProteinModelPortal:   Q6NH37
- SMR:   Q6NH37
- GeneID:   2649862
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1308
- NMPDR:   fig|257309.1.peg.1252
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   CDIP257309:DIP1308-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27300; Mature: 27169

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTPLIAGNWKMNLDHLQAVATVQKLAFALPKEYYEKVDVAVTVPFTDLRSVQTLIEGD
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECHHHHHHHHHHHCCC
KLAITYGAQDVSQHEAGAYTGEISAQMLAKLGCTWVVVGHSERREYHGESSELVAVKAKA
EEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCEEEEEEHH
ALNQGISPIVCVGEPLEIREAGTHVEYVVEQTRASLAGLTADDLAKTVIAYEPVWAIGTG
HHHCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCC
KVASAADAQEVCAAIRNLIIELAGKEVAEGLRILYGGSVKAETVAEIVGQPDVDGGLVGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEECC
ASLDGEAFAKLAANAATVVD
CCCCHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
ARTPLIAGNWKMNLDHLQAVATVQKLAFALPKEYYEKVDVAVTVPFTDLRSVQTLIEGD
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECHHHHHHHHHHHCCC
KLAITYGAQDVSQHEAGAYTGEISAQMLAKLGCTWVVVGHSERREYHGESSELVAVKAKA
EEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCEEEEEEHH
ALNQGISPIVCVGEPLEIREAGTHVEYVVEQTRASLAGLTADDLAKTVIAYEPVWAIGTG
HHHCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCC
KVASAADAQEVCAAIRNLIIELAGKEVAEGLRILYGGSVKAETVAEIVGQPDVDGGLVGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEECC
ASLDGEAFAKLAANAATVVD
CCCCHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910