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The map label for this gene is prfA

Identifier: 38233635

GI number: 38233635

Start: 1023434

End: 1024504

Strand: Direct

Name: prfA

Synonym: DIP1041

Alternate gene names: 38233635

Gene position: 1023434-1024504 (Clockwise)

Preceding gene: 38233634

Following gene: 38233636

Centisome position: 41.12

GC content: 53.97

Gene sequence:

>1071_bases
ATGTCTCAGGTTTCTGCAGTTGACGACATCGTCTCGGAATACCAAGGCATTGAAGCCCAAATGTCTGATCCAGAGACGAT
GGGTGACCAAACGCTATTCCGTAAGCTATCCAAGCGTTATTCGGAGTTGCAGCCCATCATCAACGTAAACAACAAGTTGG
TGCAGGCTCGTGACGACCACGAAGCCGCTGAGGAAATGGCCTACGAGGATAAAGAGTTCGCCGCTGAGGCCGAACGTCTT
GCCGAAGAAATCGTTTCTTTGGAAGAACAGCTCGCTGATCTGCTTGCACCACGCGATCCTCACGATGGTGATGACATTGT
GATGGAAGTCAAAGCGGGTGCCGGTGGTGAAGAAGCTGCACTTTTCGCTGGTGAACTCGTGCGTATGTACCAACGCTACG
CGGAAAAGCATGGTTTCACGGTCGAGGTTCTGGGCTTGTCTGAATCTGATTTGGGTGGCGTCAAAGATATGACCTTGTCC
ATTCGCTCGAAGACTCCATCTCGTGATGGTGCGTGGAGCGTGTTCAAGTTTGAAGGTGGTGTTCACCGCGTACAGCGTGT
GCCTGTCACTGAGTCTCAAGGCCGCATCCAAACATCGGCAGCAGGTGTACTGGTCTACCCTGAGCCCGATGAGGTAGCTG
AGGTCGAGATCGACGACAAGGATTTGCGTGTCGACGTCTACCGTTCCTCGGGTAAAGGCGGTCAGGGCGTGAACACCACA
GACTCCGCTGTGCGTATTACGCACCTTCCTACGGGGTTGGTTGTGACCTGCCAAAAGGAACGTTCGCAGATCCAGAACAA
GGCTCGTGCTATGCAGGTGCTCGCTGCTCGTCTTCAAGCTATGGCAGAAGAACAGGCAGAAGCAGAAGCAGCTGAGGGTC
GCGCTGCACAGATTCGTACTATGGACCGCTCGGAACGAATCCGTACCTACAACTGGCCAGAGAACCGCATCTCGGATCAC
CGCATTGGATACAAGGCCAATAACCTGGACTCCGTACTCAACGGTGATCTTGATGATCTGTTCTCTGCGTTGCAGGCAGC
AGAGCGTGCCGAGAGGCTCGAGGCGGAATAG

Upstream 100 bases:

>100_bases
ATTTGTTGATCAAGCAGCTCAAGAAGACCAAGTCCAATGGTGAATTCTTGATGCAGGTAGCAAGTTCTGCGCCGATGGCT
GCGGATGCTGAGGAGGACTA

Downstream 100 bases:

>100_bases
CCACGTGCTAGCTCAGGCATTACACAAGGCGGAGGCCATTCTCGCTGAGGCGGGGGTGGCCTCGCCGCGTAACGACGTCC
ACCTTATGGCGGCGCATTTA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSQVSAVDDIVSEYQGIEAQMSDPETMGDQTLFRKLSKRYSELQPIINVNNKLVQARDDHEAAEEMAYEDKEFAAEAERL
AEEIVSLEEQLADLLAPRDPHDGDDIVMEVKAGAGGEEAALFAGELVRMYQRYAEKHGFTVEVLGLSESDLGGVKDMTLS
IRSKTPSRDGAWSVFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVAEVEIDDKDLRVDVYRSSGKGGQGVNTT
DSAVRITHLPTGLVVTCQKERSQIQNKARAMQVLAARLQAMAEEQAEAEAAEGRAAQIRTMDRSERIRTYNWPENRISDH
RIGYKANNLDSVLNGDLDDLFSALQAAERAERLEAE

Sequences:

>Translated_356_residues
MSQVSAVDDIVSEYQGIEAQMSDPETMGDQTLFRKLSKRYSELQPIINVNNKLVQARDDHEAAEEMAYEDKEFAAEAERL
AEEIVSLEEQLADLLAPRDPHDGDDIVMEVKAGAGGEEAALFAGELVRMYQRYAEKHGFTVEVLGLSESDLGGVKDMTLS
IRSKTPSRDGAWSVFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVAEVEIDDKDLRVDVYRSSGKGGQGVNTT
DSAVRITHLPTGLVVTCQKERSQIQNKARAMQVLAARLQAMAEEQAEAEAAEGRAAQIRTMDRSERIRTYNWPENRISDH
RIGYKANNLDSVLNGDLDDLFSALQAAERAERLEAE
>Mature_355_residues
SQVSAVDDIVSEYQGIEAQMSDPETMGDQTLFRKLSKRYSELQPIINVNNKLVQARDDHEAAEEMAYEDKEFAAEAERLA
EEIVSLEEQLADLLAPRDPHDGDDIVMEVKAGAGGEEAALFAGELVRMYQRYAEKHGFTVEVLGLSESDLGGVKDMTLSI
RSKTPSRDGAWSVFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVAEVEIDDKDLRVDVYRSSGKGGQGVNTTD
SAVRITHLPTGLVVTCQKERSQIQNKARAMQVLAARLQAMAEEQAEAEAAEGRAAQIRTMDRSERIRTYNWPENRISDHR
IGYKANNLDSVLNGDLDDLFSALQAAERAERLEAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=43.6708860759494, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=37.0056497175141, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI166795305, Length=220, Percent_Identity=43.6363636363636, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=46.2395543175487, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.0239520958084, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=35.6363636363636, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321295, Length=328, Percent_Identity=37.5, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI19921226, Length=267, Percent_Identity=44.1947565543071, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CORDI (Q6NHU0)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939402.1
- HSSP:   Q9X183
- ProteinModelPortal:   Q6NHU0
- SMR:   Q6NHU0
- GeneID:   2648625
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1041
- NMPDR:   fig|257309.1.peg.993
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   CDIP257309:DIP1041-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39415; Mature: 39284

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVSAVDDIVSEYQGIEAQMSDPETMGDQTLFRKLSKRYSELQPIINVNNKLVQARDDH
CCCHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
EAAEEMAYEDKEFAAEAERLAEEIVSLEEQLADLLAPRDPHDGDDIVMEVKAGAGGEEAA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHH
LFAGELVRMYQRYAEKHGFTVEVLGLSESDLGGVKDMTLSIRSKTPSRDGAWSVFKFEGG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCC
VHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVAEVEIDDKDLRVDVYRSSGKGGQGVNTT
HHHHHCCCCCCCCCCEEECCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVTCQKERSQIQNKARAMQVLAARLQAMAEEQAEAEAAEGRAAQIRT
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEE
MDRSERIRTYNWPENRISDHRIGYKANNLDSVLNGDLDDLFSALQAAERAERLEAE
CCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQVSAVDDIVSEYQGIEAQMSDPETMGDQTLFRKLSKRYSELQPIINVNNKLVQARDDH
CCHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
EAAEEMAYEDKEFAAEAERLAEEIVSLEEQLADLLAPRDPHDGDDIVMEVKAGAGGEEAA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHH
LFAGELVRMYQRYAEKHGFTVEVLGLSESDLGGVKDMTLSIRSKTPSRDGAWSVFKFEGG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCC
VHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVAEVEIDDKDLRVDVYRSSGKGGQGVNTT
HHHHHCCCCCCCCCCEEECCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVTCQKERSQIQNKARAMQVLAARLQAMAEEQAEAEAAEGRAAQIRT
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEE
MDRSERIRTYNWPENRISDHRIGYKANNLDSVLNGDLDDLFSALQAAERAERLEAE
CCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910