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The map label for this gene is glcA [H]

Identifier: 38233628

GI number: 38233628

Start: 1012107

End: 1013765

Strand: Direct

Name: glcA [H]

Synonym: DIP1033

Alternate gene names: 38233628

Gene position: 1012107-1013765 (Clockwise)

Preceding gene: 38233624

Following gene: 38233629

Centisome position: 40.67

GC content: 52.56

Gene sequence:

>1659_bases
GTGTTTACACCAGTCATAGATACCGTGGGCGGTAGCCTCGGTCTCTCGGCGCTATGCGCGATCCTGCCGCTAATCGGCTT
CTTCATATTCTTGATGGTGCTCAAGATGAAAGCCCACTGGGCGGCACTCTCAAGCGTTGGAGTGGCGCTTCTTGTTGGTA
TTTTCCTCTTCAAGATGCCCGCTAACCTCGCACTTCTTTCCTTTGCTGAAGGCGTAGCTTTCGGTTTATTCCCAATCGTT
TACGTCATTTGGATGGCGGTTTGGATCTACGATCTCACCGTTAAATCAGGTCGTTTTGAAGACCTTCGTACGATCTTCTC
CAAGATCGGCCGTGGCGATATGCGAGTTCAGGCCATGCTTATTGGCTTCGCATTTGGCGGCCTATTGGAAGCACTTGCTG
GGTTCGGCGCACCTATTGCGATCGTCGCGGCAATGCTACTTGCCATTGGTCTTAAGCCGATGAAGGCTGTTCTTGTCACC
TTCGTTGCAAACTGTGCGCCTGTGGCATTCGGTGCCATGGGTATTCCTGTGACGACTGGTGGTCTTCTTACTCAGATCCC
AGGCGAGCAAGTTGCTGCTATGGCCGGACGACAGGTTTCCCTCATTGCCTTAATCGTCCCGTTCATTTTGGCACTGATCA
TGGATGGCACCCGTGGCGTTCGCCAGACTTGGCCAATGGCCCTTTTGCTGGGTATCACCTTCGGTGGTGGCCAGTTCTTG
GCATCTAATTACTTCTCCTACGTGCTTACCGACGTCGTCGCGTGCCTGCTTTCCCTCATCTGCGGTGTTGCGTTCTTGCG
TGTCTGGAAGCCAAAGACTCCAGAGGATCAAGCTTCTGAGATTGATGCCTCGACCATTCACCTGTCTGCTGATCGGGCAT
TCCTTGCGCTGTTCCCATATCTTCTCGTTGTCGTGGTGTTCTCGATCACCAGCTTGTGGAAGATAGGCGTTAACATCCCA
GCTGCTCTGAAATCGACAGATATTAAGGTTCAGTGGCCTGGACTTCATGGTCACTTGGTCAACCAAGCCGGCGAGCCAAT
TCCTAACACCATTTTTAACTTCAACTGGTTGTCTACTCCTGGCACGATCCTCTTCTTCTGCGGTTTGATCACAGTTCTCG
TGTACTCCGCAGCGGCTAAGAACAGCAATGGTGCATACCCAATGTCGTTCAAAGCGGGCTTTAGCCAATTGGCTGAAGGT
GCCTACAAGATGCGCTTCTCCGCTTTGACCATTGCTGCTGTGATGGGTCTTGCTTATGTGATGAACCTTTCAGGCCAGAC
TGCTTCTATTGGTGCCTTTTTGGCAGCTACCGGCACTATCTTCCCACTGCTTTCGCCGCTGCTGGGTTGGATTGGTACAT
GGGTGACAGGATCTGCAACTTCTGCCAATGCGTTGTTTGCGCAAATGCAGTCCACCACCGCAACCCAGGTGAGTGTAAGC
CCAACTCTACTGGTGGGTGCCAACACCGCTGGTGCAACCTTGGGCAAGATGATGAGCCCGCAGACTCTGACCATTGCTGC
CAACGCTGTCAACATGGAAAACGGCGAACGCAAGATCATGGCTCAGGCGTGGAAGTACTCCCTCGGACTTCTGGTCTACA
TCTGCATCATTGCGTACCTTCAGTCCACCCCAATTCTCGGATGGATGGTTGTGGGCTAA

Upstream 100 bases:

>100_bases
TGTTTGAAATCATAATCTTCCCCAAATATGACTTATGAGTTAAAAAAGCGCACAATAATGAGTGAACTTTTTGTCCCCCC
AATGATGGGAGTTAAGCCTC

Downstream 100 bases:

>100_bases
ACTTCTAACCCATGACACCTGCGGATCTTTCTCAACTAATTAAACAAACAGCAATTGATGTTTTGTCGGCGCGTGAGCTC
GACACCACGGTGCTCCCTGA

Product: putative L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MFTPVIDTVGGSLGLSALCAILPLIGFFIFLMVLKMKAHWAALSSVGVALLVGIFLFKMPANLALLSFAEGVAFGLFPIV
YVIWMAVWIYDLTVKSGRFEDLRTIFSKIGRGDMRVQAMLIGFAFGGLLEALAGFGAPIAIVAAMLLAIGLKPMKAVLVT
FVANCAPVAFGAMGIPVTTGGLLTQIPGEQVAAMAGRQVSLIALIVPFILALIMDGTRGVRQTWPMALLLGITFGGGQFL
ASNYFSYVLTDVVACLLSLICGVAFLRVWKPKTPEDQASEIDASTIHLSADRAFLALFPYLLVVVVFSITSLWKIGVNIP
AALKSTDIKVQWPGLHGHLVNQAGEPIPNTIFNFNWLSTPGTILFFCGLITVLVYSAAAKNSNGAYPMSFKAGFSQLAEG
AYKMRFSALTIAAVMGLAYVMNLSGQTASIGAFLAATGTIFPLLSPLLGWIGTWVTGSATSANALFAQMQSTTATQVSVS
PTLLVGANTAGATLGKMMSPQTLTIAANAVNMENGERKIMAQAWKYSLGLLVYICIIAYLQSTPILGWMVVG

Sequences:

>Translated_552_residues
MFTPVIDTVGGSLGLSALCAILPLIGFFIFLMVLKMKAHWAALSSVGVALLVGIFLFKMPANLALLSFAEGVAFGLFPIV
YVIWMAVWIYDLTVKSGRFEDLRTIFSKIGRGDMRVQAMLIGFAFGGLLEALAGFGAPIAIVAAMLLAIGLKPMKAVLVT
FVANCAPVAFGAMGIPVTTGGLLTQIPGEQVAAMAGRQVSLIALIVPFILALIMDGTRGVRQTWPMALLLGITFGGGQFL
ASNYFSYVLTDVVACLLSLICGVAFLRVWKPKTPEDQASEIDASTIHLSADRAFLALFPYLLVVVVFSITSLWKIGVNIP
AALKSTDIKVQWPGLHGHLVNQAGEPIPNTIFNFNWLSTPGTILFFCGLITVLVYSAAAKNSNGAYPMSFKAGFSQLAEG
AYKMRFSALTIAAVMGLAYVMNLSGQTASIGAFLAATGTIFPLLSPLLGWIGTWVTGSATSANALFAQMQSTTATQVSVS
PTLLVGANTAGATLGKMMSPQTLTIAANAVNMENGERKIMAQAWKYSLGLLVYICIIAYLQSTPILGWMVVG
>Mature_552_residues
MFTPVIDTVGGSLGLSALCAILPLIGFFIFLMVLKMKAHWAALSSVGVALLVGIFLFKMPANLALLSFAEGVAFGLFPIV
YVIWMAVWIYDLTVKSGRFEDLRTIFSKIGRGDMRVQAMLIGFAFGGLLEALAGFGAPIAIVAAMLLAIGLKPMKAVLVT
FVANCAPVAFGAMGIPVTTGGLLTQIPGEQVAAMAGRQVSLIALIVPFILALIMDGTRGVRQTWPMALLLGITFGGGQFL
ASNYFSYVLTDVVACLLSLICGVAFLRVWKPKTPEDQASEIDASTIHLSADRAFLALFPYLLVVVVFSITSLWKIGVNIP
AALKSTDIKVQWPGLHGHLVNQAGEPIPNTIFNFNWLSTPGTILFFCGLITVLVYSAAAKNSNGAYPMSFKAGFSQLAEG
AYKMRFSALTIAAVMGLAYVMNLSGQTASIGAFLAATGTIFPLLSPLLGWIGTWVTGSATSANALFAQMQSTTATQVSVS
PTLLVGANTAGATLGKMMSPQTLTIAANAVNMENGERKIMAQAWKYSLGLLVYICIIAYLQSTPILGWMVVG

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=560, Percent_Identity=38.5714285714286, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI1790031, Length=559, Percent_Identity=35.5992844364937, Blast_Score=298, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58430; Mature: 58430

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCEEEEEC
>Mature Secondary Structure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HCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]