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The map label for this gene is rluA [H]

Identifier: 38233570

GI number: 38233570

Start: 946181

End: 946912

Strand: Direct

Name: rluA [H]

Synonym: DIP0975

Alternate gene names: 38233570

Gene position: 946181-946912 (Clockwise)

Preceding gene: 38233569

Following gene: 38233571

Centisome position: 38.02

GC content: 56.97

Gene sequence:

>732_bases
GTGGCGGCGTCGGCAGGCGATAGTCCCTTTAGCCCAGGGACCCCGCTTGCCCCTGGCACCCTCCTAGAGAAACCCATCCC
CGCATGGTTCTGGCTAGGAGTTCCGGAAGAACCGTCAATCCCGTTCGACTACACGGTGATTGCACGCACCCCTGACCTCC
TAGTGGTGGATAAGCCGTGTTTTCTGCCGGCAACGTCGAATGGGCGAATCGTGGCAGAAACAGTGCAAACGCGCGCCCGA
ATTCGCGAAAACAACCCGCACATCGTGCCGCTACATCGTCTCGACCGCCTCACTACCGGCATTCTTTTATGCTCAACTAA
TCCCGAAACACGGGCGACTTACCAGCAGCTTTTCCAACATAGGGCGATCACCAAACGCTATATTGCCCATGTCGCGGGCC
CGATCCCGTTCGGTCCACAATGGACAGAGATCGCACTAGGAATGCGCAAAATGGGGCCGCGGGTCGTCGTCGATCCCACA
GGCACTCTTACTCGCACGAGGGTGCGTCGACTAGAAGAGGGGGTCGTTGAGCTCCAGCCGGCCACAGGCCATACTCATCA
GCTTCGCGTCCTCCTAGCGTGGGTAGGTTGTCCGATCATTGGCGACGACACATATCCCGTCGATCGGCGGCGCAGAATTT
ACGATTTTTCCAAGAAATTAGAGTTATACGCCACGTCTTTGGAGTTCGTTGATCCCGTGTCCAATGTGACTAGGCGATTC
GCTAGAGGATAA

Upstream 100 bases:

>100_bases
ACCACTGATGACAATGCCGAAAAAGTGGCGGCCCGCCTCCAAAAGGGGAATTAGTTACCAGAAGGTCGTCCTCCACGATG
TCGTCCCCGAAGGCGAGTAC

Downstream 100 bases:

>100_bases
AATCAAAAAGTTCGCTGTCTCTAGGAGGAAAGACATGACCAACCGAGCAACTGATTCAGTGCTGGGCAATCTTCGTCAGT
TCATTATGTTTGGGCTGGTT

Product: putative RNA pseudouridylate synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MAASAGDSPFSPGTPLAPGTLLEKPIPAWFWLGVPEEPSIPFDYTVIARTPDLLVVDKPCFLPATSNGRIVAETVQTRAR
IRENNPHIVPLHRLDRLTTGILLCSTNPETRATYQQLFQHRAITKRYIAHVAGPIPFGPQWTEIALGMRKMGPRVVVDPT
GTLTRTRVRRLEEGVVELQPATGHTHQLRVLLAWVGCPIIGDDTYPVDRRRRIYDFSKKLELYATSLEFVDPVSNVTRRF
ARG

Sequences:

>Translated_243_residues
MAASAGDSPFSPGTPLAPGTLLEKPIPAWFWLGVPEEPSIPFDYTVIARTPDLLVVDKPCFLPATSNGRIVAETVQTRAR
IRENNPHIVPLHRLDRLTTGILLCSTNPETRATYQQLFQHRAITKRYIAHVAGPIPFGPQWTEIALGMRKMGPRVVVDPT
GTLTRTRVRRLEEGVVELQPATGHTHQLRVLLAWVGCPIIGDDTYPVDRRRRIYDFSKKLELYATSLEFVDPVSNVTRRF
ARG
>Mature_242_residues
AASAGDSPFSPGTPLAPGTLLEKPIPAWFWLGVPEEPSIPFDYTVIARTPDLLVVDKPCFLPATSNGRIVAETVQTRARI
RENNPHIVPLHRLDRLTTGILLCSTNPETRATYQQLFQHRAITKRYIAHVAGPIPFGPQWTEIALGMRKMGPRVVVDPTG
TLTRTRVRRLEEGVVELQPATGHTHQLRVLLAWVGCPIIGDDTYPVDRRRRIYDFSKKLELYATSLEFVDPVSNVTRRFA
RG

Specific function: Responsible for synthesis of pseudouridine from uracil- 746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=166, Percent_Identity=33.1325301204819, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17534965, Length=181, Percent_Identity=35.3591160220994, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324506, Length=178, Percent_Identity=29.7752808988764, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320168, Length=176, Percent_Identity=28.9772727272727, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921090, Length=166, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583449, Length=160, Percent_Identity=33.125, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583447, Length=160, Percent_Identity=33.125, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583445, Length=160, Percent_Identity=33.125, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27195; Mature: 27064

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASAGDSPFSPGTPLAPGTLLEKPIPAWFWLGVPEEPSIPFDYTVIARTPDLLVVDKPC
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCE
FLPATSNGRIVAETVQTRARIRENNPHIVPLHRLDRLTTGILLCSTNPETRATYQQLFQH
EEEECCCCEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
RAITKRYIAHVAGPIPFGPQWTEIALGMRKMGPRVVVDPTGTLTRTRVRRLEEGVVELQP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCEEEEC
ATGHTHQLRVLLAWVGCPIIGDDTYPVDRRRRIYDFSKKLELYATSLEFVDPVSNVTRRF
CCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
ARG
HCC
>Mature Secondary Structure 
AASAGDSPFSPGTPLAPGTLLEKPIPAWFWLGVPEEPSIPFDYTVIARTPDLLVVDKPC
CCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCE
FLPATSNGRIVAETVQTRARIRENNPHIVPLHRLDRLTTGILLCSTNPETRATYQQLFQH
EEEECCCCEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
RAITKRYIAHVAGPIPFGPQWTEIALGMRKMGPRVVVDPTGTLTRTRVRRLEEGVVELQP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCEEEEC
ATGHTHQLRVLLAWVGCPIIGDDTYPVDRRRRIYDFSKKLELYATSLEFVDPVSNVTRRF
CCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
ARG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]