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The map label for this gene is ispH

Identifier: 38233539

GI number: 38233539

Start: 914162

End: 915163

Strand: Direct

Name: ispH

Synonym: DIP0943

Alternate gene names: 38233539

Gene position: 914162-915163 (Clockwise)

Preceding gene: 38233536

Following gene: 38233544

Centisome position: 36.73

GC content: 55.79

Gene sequence:

>1002_bases
GTGTGCATGACTTCACCCGATCAGCTCACCGCTTCTGAGTCCGCTGACACCGCCTCCGCACACAAGCGTGTGCTTGTCGC
AGCCCCACGAGGCTACTGCGCCGGCGTGGATCGCGCTGTCGAAACCGTCGAAAAGGCCCTAGAAAAATATGGTGCGCCCG
TCTACGTGCGTAAAGAGATCGTCCACAATCGTTACGTTGTGGACACACTCGCCGAACGCGGCGCCATCTTTGTCGATGAG
ACAACCGAAGCTCCAGAAGGTGCGCATCTAGTATTTTCTGCTCATGGCGTCAGCCCCGCCGTACACAAAGAGGCAGAAGC
ACTGAGCCTGAAAACTCTTGACGCAACGTGCCCCTTGGTCACCAAAGTGCACAACGAGGTGAAACGGTTTGCACGCGACG
GCTACCACATCCTCCTAGTCGGGCACGAAGGCCATGAGGAAGTTGAAGGCACTGCAGGCGAGGCCCCTGACGTGACCCAC
CTTGTCGACGGAGTTGAAAGCGTGGACAAACTCCCCGAATTTCTTCACGACGAAAAACTCATCTGGCTTTCGCAAACCAC
GTTATCCGTGGACGAAACCATGACGATCGTGAAAAAGCTACACGAGCGCTTCGCACACCTCCAAGACCCTCCTAGTGATG
ACATCTGCTATGCCACCCAAAACCGCCAAGTGGCAGTCAAAGCAATTGCTGCAGAATCGGATGTCGTCATCGTTGTCGGT
TCCCAGAACTCCTCGAACTCCAAACGACTGGTTGAAGTGGCCCTACAAGCCGGTGCTGGCGCGTCCTATCTGGTTGACTA
CGCCCACCAGATTGATGAGTCTTGGCTCGATGGTGCCACCACCGTGGGTGTGTCTTCAGGCGCTTCCGTGCCAGAGATTT
TGGTGCGCGGAGTACTGGAATTTCTTGACGCGCGTGGTTTCCATGACGTTAAGGAGATCACTACTGCGGCAGAAAAGATT
ACCTTCGCCCTTCCTAGAGATTTGCGCCCTGCGCGCACATAG

Upstream 100 bases:

>100_bases
AACCCCCTTCTTCACGGCGAGCAACAAACTGTATTCAACAGGAACCAACACACAAGCATACGGCAGGAGGGAGCACTCAG
TGGGACAGTAGTTTAAGCTG

Downstream 100 bases:

>100_bases
AGCGAAGCAGAAGGCGCCACGCATGGGATATGCGTGGCGCCTTGTGCGTTCTAACTTTCGACTTTAACTGTATAAGTCGT
CGTCGAAAGTGCGAGTTCTA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MCMTSPDQLTASESADTASAHKRVLVAAPRGYCAGVDRAVETVEKALEKYGAPVYVRKEIVHNRYVVDTLAERGAIFVDE
TTEAPEGAHLVFSAHGVSPAVHKEAEALSLKTLDATCPLVTKVHNEVKRFARDGYHILLVGHEGHEEVEGTAGEAPDVTH
LVDGVESVDKLPEFLHDEKLIWLSQTTLSVDETMTIVKKLHERFAHLQDPPSDDICYATQNRQVAVKAIAAESDVVIVVG
SQNSSNSKRLVEVALQAGAGASYLVDYAHQIDESWLDGATTVGVSSGASVPEILVRGVLEFLDARGFHDVKEITTAAEKI
TFALPRDLRPART

Sequences:

>Translated_333_residues
MCMTSPDQLTASESADTASAHKRVLVAAPRGYCAGVDRAVETVEKALEKYGAPVYVRKEIVHNRYVVDTLAERGAIFVDE
TTEAPEGAHLVFSAHGVSPAVHKEAEALSLKTLDATCPLVTKVHNEVKRFARDGYHILLVGHEGHEEVEGTAGEAPDVTH
LVDGVESVDKLPEFLHDEKLIWLSQTTLSVDETMTIVKKLHERFAHLQDPPSDDICYATQNRQVAVKAIAAESDVVIVVG
SQNSSNSKRLVEVALQAGAGASYLVDYAHQIDESWLDGATTVGVSSGASVPEILVRGVLEFLDARGFHDVKEITTAAEKI
TFALPRDLRPART
>Mature_333_residues
MCMTSPDQLTASESADTASAHKRVLVAAPRGYCAGVDRAVETVEKALEKYGAPVYVRKEIVHNRYVVDTLAERGAIFVDE
TTEAPEGAHLVFSAHGVSPAVHKEAEALSLKTLDATCPLVTKVHNEVKRFARDGYHILLVGHEGHEEVEGTAGEAPDVTH
LVDGVESVDKLPEFLHDEKLIWLSQTTLSVDETMTIVKKLHERFAHLQDPPSDDICYATQNRQVAVKAIAAESDVVIVVG
SQNSSNSKRLVEVALQAGAGASYLVDYAHQIDESWLDGATTVGVSSGASVPEILVRGVLEFLDARGFHDVKEITTAAEKI
TFALPRDLRPART

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=51.2903225806452, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CORDI (Q6NI36)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939306.1
- GeneID:   2649983
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0943
- NMPDR:   fig|257309.1.peg.897
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q6NI36
- ProtClustDB:   PRK01045
- BioCyc:   CDIP257309:DIP0943-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36051; Mature: 36051

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMTSPDQLTASESADTASAHKRVLVAAPRGYCAGVDRAVETVEKALEKYGAPVYVRKEI
CCCCCCCCCCCCCCCCHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
VHNRYVVDTLAERGAIFVDETTEAPEGAHLVFSAHGVSPAVHKEAEALSLKTLDATCPLV
HHCHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHEEEECCCCCHHH
TKVHNEVKRFARDGYHILLVGHEGHEEVEGTAGEAPDVTHLVDGVESVDKLPEFLHDEKL
HHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IWLSQTTLSVDETMTIVKKLHERFAHLQDPPSDDICYATQNRQVAVKAIAAESDVVIVVG
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEE
SQNSSNSKRLVEVALQAGAGASYLVDYAHQIDESWLDGATTVGVSSGASVPEILVRGVLE
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
FLDARGFHDVKEITTAAEKITFALPRDLRPART
HHHCCCCHHHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MCMTSPDQLTASESADTASAHKRVLVAAPRGYCAGVDRAVETVEKALEKYGAPVYVRKEI
CCCCCCCCCCCCCCCCHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
VHNRYVVDTLAERGAIFVDETTEAPEGAHLVFSAHGVSPAVHKEAEALSLKTLDATCPLV
HHCHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHEEEECCCCCHHH
TKVHNEVKRFARDGYHILLVGHEGHEEVEGTAGEAPDVTHLVDGVESVDKLPEFLHDEKL
HHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IWLSQTTLSVDETMTIVKKLHERFAHLQDPPSDDICYATQNRQVAVKAIAAESDVVIVVG
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEE
SQNSSNSKRLVEVALQAGAGASYLVDYAHQIDESWLDGATTVGVSSGASVPEILVRGVLE
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
FLDARGFHDVKEITTAAEKITFALPRDLRPART
HHHCCCCHHHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910